Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA97.6% 412.1 / ‑0.3 127C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (69/55);  total (71/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

Only 100 of 129 total aligned reads displayed.

ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162876‑163069
                                                                                                 |                                                                                                
aTCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtatg                                                                                                <  1:2902806/100‑1 (MQ=255)
 tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  2:2633239/100‑1 (MQ=255)
 gatctccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  1:2402184/95‑1 (MQ=255)
   ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  1:599046/1‑100 (MQ=255)
   ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  2:2193355/1‑100 (MQ=255)
   ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              >  1:2350741/1‑99 (MQ=255)
    tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGACTATGCCCa                                                                                            >  1:2945359/1‑100 (MQ=255)
     cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAg                                                                                           <  2:968233/100‑1 (MQ=255)
     cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAACATTGCTGAAACCAGTTTAACCCTTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  1:2883514/1‑98 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcagatc                                                                                          >  2:2402201/1‑95 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAgg                                                                                          >  2:866312/1‑100 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAgg                                                                                          >  1:938442/1‑100 (MQ=255)
        ggCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAg                                                                                        <  1:2233624/100‑1 (MQ=255)
        ggCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAg                                                                                        <  1:916425/100‑1 (MQ=255)
          cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                      >  1:1785087/1‑100 (MQ=255)
            ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    <  2:2788405/100‑1 (MQ=255)
            ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    >  1:2010619/1‑100 (MQ=255)
             cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtgt                                                                                   <  2:3261890/100‑1 (MQ=255)
             cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtat                                                                                   <  2:2304204/100‑3 (MQ=255)
              gCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGtgtg                                                                                  >  2:531272/1‑100 (MQ=255)
              gCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGtgtg                                                                                  >  1:1492597/1‑100 (MQ=255)
               cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                 <  2:3052003/100‑1 (MQ=255)
                 aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcg                                                                               <  2:3125606/100‑1 (MQ=255)
                 aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcg                                                                               <  1:1192742/100‑1 (MQ=255)
                  gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgcgc                                                                              >  2:2759866/1‑100 (MQ=255)
                  gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcgc                                                                              >  2:2494311/1‑100 (MQ=255)
                  gggATGCGCAAAGTGGTGCTGGCGACCAAAATTGCTGAAACCAGGTTAACCATTGAAGGGATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcgc                                                                              >  1:1456249/1‑100 (MQ=255)
                   ggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGcgcg                                                                             <  1:37019/100‑1 (MQ=255)
                     aTGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtt                                                                           <  1:849300/100‑1 (MQ=255)
                      tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                          >  1:1838169/1‑100 (MQ=255)
                      tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                          >  1:1359551/1‑100 (MQ=255)
                       tcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  2:802322/99‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  2:1971084/100‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:2219210/100‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:1365448/100‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:233146/100‑1 (MQ=255)
                       gcgcAAAGTGGGGCTGGCGACCACTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:1660029/100‑1 (MQ=255)
                        cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttta                                                                        >  1:802319/1‑99 (MQ=255)
                        cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                        <  2:2030326/100‑1 (MQ=255)
                         gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       >  2:2922197/1‑100 (MQ=255)
                         gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       >  1:1689832/1‑100 (MQ=255)
                           aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                     <  2:3381400/100‑1 (MQ=255)
                             aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGACCAGGTGCGTGTGGCGCGTTTTGATCcg                                                                   <  1:598638/100‑1 (MQ=255)
                              gtggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgc                                                                  >  2:2916177/1‑100 (MQ=255)
                               tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                 <  2:3002364/100‑1 (MQ=255)
                                ggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCgcgc                                                                >  1:1214136/1‑100 (MQ=255)
                                    cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgg                                                            >  1:1496641/1‑100 (MQ=255)
                                      ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                          >  2:2128431/1‑100 (MQ=255)
                                      ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                          >  2:367/1‑100 (MQ=255)
                                      ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCATGTGGCGCGTTTTGATCCGCGCACgggg                                                          >  2:262699/1‑100 (MQ=255)
                                         gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCTCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGTGCtt                                                       >  1:2554020/1‑100 (MQ=255)
                                          aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                      >  1:3041378/1‑100 (MQ=255)
                                          aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                      <  1:1595546/100‑1 (MQ=255)
                                             aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                   <  2:2321484/100‑1 (MQ=255)
                                              atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  <  2:2019071/100‑1 (MQ=255)
                                               tatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGa                                                 >  2:2504861/1‑100 (MQ=255)
                                               tatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGa                                                 >  1:2556519/1‑100 (MQ=255)
                                               tatTGCGGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                   <  2:3051632/98‑1 (MQ=255)
                                                atTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                                <  1:1478534/100‑1 (MQ=255)
                                                   gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  2:3116246/1‑100 (MQ=255)
                                                     tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           >  1:1107490/1‑100 (MQ=255)
                                                      gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                          >  1:299213/1‑100 (MQ=255)
                                                      gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                          >  2:3132311/1‑100 (MQ=255)
                                                       aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                         <  2:1037318/100‑1 (MQ=255)
                                                        aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                        >  2:2731451/1‑100 (MQ=255)
                                                        aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                        >  2:404759/1‑100 (MQ=255)
                                                         aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                       <  2:2148598/100‑1 (MQ=255)
                                                          ccAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa                                      >  2:3018213/1‑100 (MQ=255)
                                                            aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                    <  1:416120/100‑1 (MQ=255)
                                                            aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                    >  1:1600422/1‑100 (MQ=255)
                                                               ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                 <  1:942971/100‑1 (MQ=255)
                                                                tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                >  1:249571/1‑100 (MQ=255)
                                                                tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                >  1:1953464/1‑100 (MQ=255)
                                                                 aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                               <  1:63024/100‑1 (MQ=255)
                                                                 aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                               <  1:2193340/100‑1 (MQ=255)
                                                                  aCCATTGAAGGTATTCGTCTGGTGGTGGCTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACCGATTACGCAACGCGt                                >  1:3283990/1‑98 (MQ=255)
                                                                    cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                            >  2:1061028/1‑100 (MQ=255)
                                                                     aTTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc                           <  2:592914/100‑1 (MQ=255)
                                                                     aTTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc                           <  2:1458471/100‑1 (MQ=255)
                                                                      ttGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCa                          >  2:1591379/1‑100 (MQ=255)
                                                                      ttGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCa                          <  2:1178440/100‑1 (MQ=255)
                                                                       tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                         >  1:51135/1‑100 (MQ=255)
                                                                       tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                         >  1:3203272/1‑100 (MQ=255)
                                                                        naaGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  1:32025/2‑100 (MQ=255)
                                                                        gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        <  1:1344014/100‑1 (MQ=255)
                                                                          aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                      <  2:2436454/100‑1 (MQ=255)
                                                                           ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt                     >  1:2603022/1‑100 (MQ=255)
                                                                            gTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATc                    <  1:3305252/100‑1 (MQ=255)
                                                                            gTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCAACTGATTACTCAACGCGTTAGCCAGGCAt                     <  1:2819566/99‑1 (MQ=255)
                                                                             tATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                   >  1:1065915/1‑100 (MQ=255)
                                                                              aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                  >  2:2424787/1‑100 (MQ=255)
                                                                                       gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc         >  1:2302606/1‑100 (MQ=255)
                                                                                       gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc         >  2:3013756/1‑100 (MQ=255)
                                                                                       gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc         >  2:1979995/1‑100 (MQ=255)
                                                                                         ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  1:1700674/1‑100 (MQ=255)
                                                                                         ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  2:2790442/1‑100 (MQ=255)
                                                                                          gtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTg      <  2:337431/100‑1 (MQ=255)
                                                                                             gATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCg   <  1:1592027/100‑1 (MQ=255)
                                                                                              aTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  >  2:2778429/1‑100 (MQ=255)
                                                                                              aTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  <  1:57874/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162876‑163069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑