BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,254,980	A→G	23.3%	F325L (TTT→CTT)	ycgV ←	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,254,980	0	A	G	23.3%	221.2 / 66.4	120	F325L (TTT→CTT)	ycgV	putative adhesin
Reads supporting (aligned to +/- strand): ref base A (42/49); new base G (13/15); total (56/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAC  >  NC_000913/1254882‑1255076

ggTATTTTGCATGTTGACAACAGCCTTTGCCGTCTGGGCGGAGGCAGCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTaaaa                                                                                                 >  1:506322/1‑100 (MQ=255)

 gTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAAt                                                                                                <  2:907240/100‑1 (MQ=255)

   aTTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTg                                                                                              >  1:1592568/1‑100 (MQ=255)

    ttttGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGACCCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTg                                                                                              <  1:131792/99‑1 (MQ=255)

    ttttGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGa                                                                                             <  2:1447220/100‑1 (MQ=255)

     tttGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGCAAAGATGCTGTTTCGTTGCGCTGCCTTGCCAGTAAAATTGAt                                                                                            >  1:2128952/1‑100 (MQ=255)

     tttGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGAt                                                                                            >  1:1040461/1‑100 (MQ=255)

     tttGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGAt                                                                                            >  1:1040462/1‑100 (MQ=255)

       tGCATGTTGAGAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGGCGGAAAAGATGCTGTTTTGTTGAGGTGCCGTGCGAGTAACAtt                                                                                               >  1:73017/1‑95 (MQ=255)

         cATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATc                                                                                        >  1:2327256/1‑100 (MQ=255)

          aTGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATcg                                                                                       <  2:930124/100‑1 (MQ=255)

           tGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATcgc                                                                                      <  2:2684139/100‑1 (MQ=255)

            gTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATcgcg                                                                                     >  1:2886254/1‑100 (MQ=255)

            gTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATcgc                                                                                      >  1:1999082/1‑99 (MQ=255)

               gACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGTGTc                                                                                   >  1:1979863/1‑99 (MQ=255)

                acaacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTg                                                                                 <  1:1191166/100‑1 (MQ=255)

                 caacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGa                                                                                >  2:1295687/1‑100 (MQ=255)

                  aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGaa                                                                               <  1:1456437/100‑1 (MQ=255)

                  aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGaa                                                                               >  2:515111/1‑100 (MQ=255)

                  aacaGCCGTTGCCGTCTACGCCGAGGCAACATAAGGACCGCCGAAAAAGATGCTGTTTCGTTGCACTGCCGTGCCAGTAAGATTGATTATCGCGTCTGaa                                                                               <  1:286675/100‑1 (MQ=255)

                   acaGCCGTTGCCGTCTGCGCCGAGGCACCATAATAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAAc                                                                              <  1:818593/100‑1 (MQ=255)

                    caGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAAcc                                                                             >  1:2672737/1‑100 (MQ=255)

                         gTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTgg                                                                        <  1:513281/100‑1 (MQ=255)

                          ttGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGt                                                                       <  2:2675740/100‑1 (MQ=255)

                           tGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTg                                                                      <  2:1651659/100‑1 (MQ=255)

                            gCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGa                                                                     <  1:2425828/100‑1 (MQ=255)

                              cGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACg                                                                   <  2:1576844/100‑1 (MQ=255)

                              cGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACg                                                                   <  2:838823/100‑1 (MQ=255)

                                tCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAg                                                                 <  2:3006408/100‑1 (MQ=255)

                                 cTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGacg                                                                >  1:1330151/1‑98 (MQ=255)

                                 cTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAgg                                                                >  2:2586634/1‑100 (MQ=255)

                                   gggCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGcc                                                              >  1:2703442/1‑100 (MQ=255)

                                   gggCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGcc                                                              >  2:1523488/1‑100 (MQ=255)

                                   gggCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGcc                                                              >  2:1481611/1‑100 (MQ=255)

                                    ggCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCa                                                             >  2:1003954/1‑100 (MQ=255)

                                     gCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCAc                                                            <  2:1592575/100‑1 (MQ=255)

                                     gCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCGGAACCACTGGTGACGAGGCCa                                                             >  1:2719431/1‑99 (MQ=255)

                                      ccGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCAcc                                                           >  2:1523476/1‑100 (MQ=255)

                                      ccGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCAcc                                                           >  2:359846/1‑100 (MQ=255)

                                      ccGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCAcc                                                           <  2:2536466/100‑1 (MQ=255)

                                      ccGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCAc                                                            >  1:2794282/1‑99 (MQ=255)

                                       cGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCg                                                          >  2:670391/1‑100 (MQ=255)

                                           gCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCt                                                      >  2:2021238/1‑100 (MQ=255)

                                              ccATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTgcg                                                   >  2:788584/1‑100 (MQ=255)

                                              ccATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTgcg                                                   >  1:656975/1‑100 (MQ=255)

                                               cATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTgcgc                                                  <  1:3013385/100‑1 (MQ=255)

                                               cATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGACTATCGCGTCTGAACCACTGGTGACGGGGCCACCGCCCTgcgc                                                  >  2:1408217/1‑100 (MQ=255)

                                                aTAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCCCTGGTGACGAGGCCACCGCCCTGcgcg                                                 <  2:221704/100‑1 (MQ=255)

                                                aTAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGcgcg                                                 <  2:1709540/100‑1 (MQ=255)

                                                 tAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCgg                                                <  1:2967362/100‑1 (MQ=255)

                                                 tAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCgg                                                <  2:1922137/100‑1 (MQ=255)

                                                  aaGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGcgcg                                                 <  1:1730697/98‑1 (MQ=255)

                                                  aaGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGga                                               <  1:1433732/100‑1 (MQ=255)

                                                   aGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGgaa                                              >  2:382012/1‑100 (MQ=255)

                                                    gagccgccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGgaag                                             <  2:3171547/97‑1 (MQ=255)

                                                     atctgccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGgaaga                                            <  1:3377486/96‑1 (MQ=255)

                                                       ccgccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGaaa                                          >  1:2571891/1‑100 (MQ=255)

                                                          ccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAatat                                       >  2:2931823/1‑100 (MQ=255)

                                                          ccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAatat                                       <  2:2762164/100‑1 (MQ=255)

                                                          ccgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAatat                                       >  1:2830872/1‑100 (MQ=255)

                                                           cgGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATg                                      <  2:3332183/100‑1 (MQ=255)

                                                           cgGAAAAGATGCTGTTTCGTTGCGCGGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATg                                      <  1:1894529/100‑1 (MQ=255)

                                                            ggAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATgg                                     >  1:2645985/1‑100 (MQ=255)

                                                            ggAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATgg                                     <  1:1407575/100‑1 (MQ=255)

                                                             gAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGc                                    <  1:456607/100‑1 (MQ=255)

                                                             gAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGc                                    <  1:2586686/100‑1 (MQ=255)

                                                              aaaaGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCt                                   <  2:750929/100‑1 (MQ=255)

                                                              aaaaGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCt                                   <  2:2838361/100‑1 (MQ=255)

                                                                 aGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATc                                <  2:964359/100‑1 (MQ=255)

                                                                 aGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATc                                <  1:788581/100‑1 (MQ=255)

                                                                  gATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCt                               <  2:902616/100‑1 (MQ=255)

                                                                  gATGCTGTTACGTCGCGCTGCCGTGCCAGCAAGACTGATTCTCGCGCCTGAACCACTGGTCACGACGCCACCGCCCTTCGCGGAAGAAATATGGc                                    <  1:2978984/95‑1 (MQ=255)

                                                                   aTGCTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTg                              <  2:1956824/100‑1 (MQ=255)

                                                                    tGCTGTTTCGTTGCGCTGCCGTGGCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGc                             <  1:2271221/100‑1 (MQ=255)

                                                                      cTGTTTCGTTGCGCTGCCGTGCCAGTAAGATTGCTTATCGCGGCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCAc                           >  1:1213330/1‑100 (MQ=255)

                                                                       tGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCAcc                          <  1:2747375/100‑1 (MQ=255)

                                                                       tGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCAcc                          >  1:890150/1‑100 (MQ=255)

                                                                        gTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCg                         >  2:422374/1‑100 (MQ=255)

                                                                        gTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCg                         >  1:2428439/1‑100 (MQ=255)

                                                                        gTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCg                         >  2:2813305/1‑100 (MQ=255)

                                                                            cGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAg                     >  2:2793815/1‑100 (MQ=255)

                                                                             gTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGt                    >  2:6880/1‑100 (MQ=255)

                                                                              ttGCGCTGCCGTGCCAGTAAGATTGATTATCGCGTCAGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTCTCTGCACCGATAGt                    <  2:56333/99‑1 (MQ=255)

                                                                              ttGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTg                   <  1:103328/100‑1 (MQ=255)

                                                                               tGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTgg                  <  2:1674730/100‑1 (MQ=255)

                                                                                gcgcTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGt                 <  2:2397604/100‑1 (MQ=255)

                                                                                 cgcTGCCGTGCCAGTAAGATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGtt                >  2:2566267/1‑100 (MQ=255)

                                                                                 cgcTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGtt                >  2:2102084/1‑100 (MQ=255)

                                                                                  gcTGGCGTGCCAGTAAGATTGATTATCGGGTCTGAACCACTGGTGACGAGGCCACCGTCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTg               <  1:270261/100‑1 (MQ=255)

                                                                                  gcTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTg               <  2:2095639/100‑1 (MQ=255)

                                                                                     gCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTac            >  2:2364996/1‑100 (MQ=255)

                                                                                         tGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTaccacc        <  2:2251787/100‑1 (MQ=255)

                                                                                          gCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTAccacca       >  2:1332579/1‑100 (MQ=255)

                                                                                           ccAGTAAAATTGATGATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACcaccac      <  2:3295565/100‑1 (MQ=255)

                                                                                            cAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACg     <  2:1028679/100‑1 (MQ=255)

                                                                                             aGTAAAATTGATTATCGCGTCTGAACCACTGGTGCCAAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGa    >  1:2351539/1‑100 (MQ=255)

                                                                                               tAAAATTGATTATCGCGTCTGAACCACTTGTGACGAGGCCACCGTCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAc  <  1:82953/100‑1 (MQ=255)

                                                                                               tAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAc  <  1:83331/100‑1 (MQ=255)

                                                                                               tAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAc  <  1:2314756/100‑1 (MQ=255)

                                                                                               tAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAc  <  1:980199/100‑1 (MQ=255)

GGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAAC  >  NC_000913/1254882‑1255076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`