BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,307,634	T→A	48.0%	I338F (ATC→TTC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,634	0	T	A	48.0%	‑2.4 / 191.8	123	I338F (ATC→TTC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base T (37/27); new base A (31/28); total (68/55)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

Only 100 of 123 total aligned reads displayed.

TCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGA  >  NC_000913/1307536‑1307731

tCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATc                                                                                                  <  1:1024428/100‑1 (MQ=255)

  gcgGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCaa                                                                                                <  2:3149145/100‑1 (MQ=255)

   cgGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACaaa                                                                                               <  2:2938360/100‑1 (MQ=255)

    ggAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAat                                                                                              <  1:2241332/100‑1 (MQ=255)

     gAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAata                                                                                             >  1:828062/1‑100 (MQ=255)

       aGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATAtt                                                                                           <  1:449137/100‑1 (MQ=255)

        ggATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTc                                                                                          >  2:397458/1‑100 (MQ=255)

         gATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTc                                                                                          >  2:736058/1‑99 (MQ=255)

         gATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCa                                                                                         <  2:2068854/100‑1 (MQ=255)

           taataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcg                                                                                       <  2:2464724/100‑1 (MQ=255)

             ataCTGACAGCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTATATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgcg                                                                                     <  1:182269/100‑1 (MQ=255)

              taCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACgcgc                                                                                    >  2:1609801/1‑100 (MQ=255)

               aCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGcc                                                                                   >  2:1501299/1‑100 (MQ=255)

               aCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGcc                                                                                   <  2:1156713/100‑1 (MQ=255)

                cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgc                                                                                      >  1:1146177/1‑96 (MQ=255)

                cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCg                                                                                  >  2:1440725/1‑100 (MQ=255)

                cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCg                                                                                  >  2:1358157/1‑100 (MQ=255)

                  gACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGaa                                                                                <  2:3030861/100‑1 (MQ=255)

                      nccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAg                                                                            >  1:845446/2‑100 (MQ=255)

                       ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGc                                                                           <  1:553347/100‑1 (MQ=255)

                       ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGc                                                                           <  2:823194/100‑1 (MQ=255)

                       ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGc                                                                           <  2:182860/100‑1 (MQ=255)

                       ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGc                                                                           >  2:567999/1‑100 (MQ=255)

                        cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGcc                                                                          <  1:431209/100‑1 (MQ=255)

                         aCCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGc                                                                           >  1:1431484/1‑98 (MQ=255)

                         aCCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCg                                                                         >  1:994499/1‑100 (MQ=255)

                          ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc                                                                        <  2:3389128/100‑1 (MQ=255)

                          ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc                                                                        <  1:1407886/100‑1 (MQ=255)

                          ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGc                                                                        >  1:1500574/1‑100 (MQ=255)

                          ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGc                                                                        >  2:1861901/1‑100 (MQ=255)

                          ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTCGTCATGAACAAATATTCACGCGCCGAATAAGCCGc                                                                        >  1:1871600/1‑100 (MQ=255)

                            ccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAg                                                                      <  2:3037808/100‑1 (MQ=255)

                               cgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCa                                                                   >  2:586060/1‑100 (MQ=255)

                               cgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCa                                                                   >  1:479433/1‑100 (MQ=255)

                                 cTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCaat                                                                 <  1:1265153/100‑1 (MQ=255)

                                 cTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCaat                                                                 <  1:2411644/100‑1 (MQ=255)

                                   gcgcCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCaataa                                                               >  1:704122/1‑100 (MQ=255)

                                   gcgcCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCaataa                                                               >  1:1590556/1‑100 (MQ=255)

                                     gccgccGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATg                                                             >  2:3308666/1‑100 (MQ=255)

                                      ccgccgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGc                                                            >  1:1992992/1‑100 (MQ=255)

                                        gccgTGCAAATCGCAGGAAGTAAATCATCGCTTGACACAAAGTAGGGCGTGGTCATAATCAAATATTCACTCGCCGAATAAGCCGCAGTCaataat                                                              <  1:2136954/96‑1 (MQ=255)

                                          cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                        >  2:1381749/1‑100 (MQ=255)

                                           gTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGt                                                       >  2:1418211/1‑100 (MQ=255)

                                            tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                      <  1:1687455/100‑1 (MQ=255)

                                            tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                      <  2:2033371/100‑1 (MQ=255)

                                            tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                      <  1:1905418/100‑1 (MQ=255)

                                               aaaTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAAt                                                   >  2:1649184/1‑100 (MQ=255)

                                                    gCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGAt                                              <  1:309454/100‑1 (MQ=255)

                                                     cATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGAt                                              >  1:2413553/1‑99 (MQ=255)

                                                      aTGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCt                                            <  2:1812739/100‑1 (MQ=255)

                                                      aTGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCt                                            <  2:1123064/100‑1 (MQ=255)

                                                         aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTcc                                         <  1:1440719/100‑1 (MQ=255)

                                                         aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAATAACTATACACGCGCCGAATAATCCGCAGGGCATACTGCCGCGTGACTGCGAACttg                                          >  1:2661794/1‑98 (MQ=255)

                                                         aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTcc                                         >  1:1503640/1‑100 (MQ=255)

                                                          aGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCg                                        <  2:3206565/100‑1 (MQ=255)

                                                          aGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCg                                        <  1:2599102/100‑1 (MQ=255)

                                                          aGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCg                                        <  2:1896117/100‑1 (MQ=255)

                                                           gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                       >  2:1088975/1‑100 (MQ=255)

                                                             aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGaa                                     >  2:337711/1‑100 (MQ=255)

                                                             aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGaa                                     >  2:771298/1‑100 (MQ=255)

                                                               atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAg                                   <  2:1121992/100‑1 (MQ=255)

                                                               atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAg                                   >  2:2379279/1‑100 (MQ=255)

                                                               atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAg                                   >  2:3160640/1‑100 (MQ=255)

                                                                   tcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCgg                               >  1:161782/1‑100 (MQ=255)

                                                                    cGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAGTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCggg                              >  1:686142/1‑100 (MQ=255)

                                                                    cGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCggg                              >  2:1968400/1‑100 (MQ=255)

                                                                     gCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCgggg                             <  1:51672/100‑1 (MQ=255)

                                                                     gCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCgggg                             <  2:3375754/100‑1 (MQ=255)

                                                                     gCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCgggg                             >  1:2660454/1‑100 (MQ=255)

                                                                      cTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGc                            <  2:1626256/100‑1 (MQ=255)

                                                                      cTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGc                            <  2:1641049/100‑1 (MQ=255)

                                                                         ggCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAg                         <  1:236672/100‑1 (MQ=255)

                                                                          gCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                        >  2:2044990/1‑100 (MQ=255)

                                                                           cacaAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                       >  2:122961/1‑100 (MQ=255)

                                                                           cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                       >  2:2978887/1‑100 (MQ=255)

                                                                           cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                       >  1:1082313/1‑100 (MQ=255)

                                                                           cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                       >  2:2387057/1‑100 (MQ=255)

                                                                           cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                       >  2:2438740/1‑100 (MQ=255)

                                                                            acaAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                      >  1:235066/1‑100 (MQ=255)

                                                                            acaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATGATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                      >  1:2371074/1‑100 (MQ=255)

                                                                              aaaGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCa                    <  2:926076/100‑1 (MQ=255)

                                                                               aaGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCaa                   >  1:2766854/1‑100 (MQ=255)

                                                                               aaGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCaa                   >  1:2134328/1‑100 (MQ=255)

                                                                                 gTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAAtt                 >  1:651330/1‑100 (MQ=255)

                                                                                   aGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgt               >  1:2773101/1‑100 (MQ=255)

                                                                                    gggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg              >  1:457681/1‑100 (MQ=255)

                                                                                    gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg              >  1:1426283/1‑100 (MQ=255)

                                                                                    gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg              <  2:651333/100‑1 (MQ=255)

                                                                                     ggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgt             >  1:777403/1‑100 (MQ=255)

                                                                                     ggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgt             >  2:2658907/1‑100 (MQ=255)

                                                                                      gCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgtg            <  2:1311208/100‑1 (MQ=255)

                                                                                      gCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgtg            <  1:2515711/100‑1 (MQ=255)

                                                                                       cGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGa           <  1:2303028/100‑1 (MQ=255)

                                                                                           gTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATgg       >  2:2856513/1‑100 (MQ=255)

                                                                                             cATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGGCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtg     <  2:2599002/100‑1 (MQ=255)

                                                                                             cATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtg     >  1:287010/1‑100 (MQ=255)

                                                                                               tGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGtgtg   >  1:876302/1‑100 (MQ=255)

                                                                                               tGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGtgtg   >  2:1449508/1‑100 (MQ=255)

                                                                                               tGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGtgtg   >  2:1431954/1‑100 (MQ=255)

                                                                                                gATCAAATGTTCATGCGCTGAATCAGCCGCAGTCGATAATGCCTGGTGAATGAGATCTCGCGGACAGCCGGGGCCAGAAGCTATTGTGTGAATGGTGTGa  <  1:1229686/100‑1 (MQ=255)

TCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGA  >  NC_000913/1307536‑1307731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`