Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,325,850 C→T 18.2% R369C (CGC→TGC)  yciQ → enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,8500CT18.2% 254.7 / 44.4 115R369C (CGC→TGC) yciQenhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (54/40);  new base T (12/9);  total (66/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 118 total aligned reads displayed.

GAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGG  >  NC_000913/1325751‑1325948
                                                                                                   |                                                                                                   
gAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGAc                                                                                                     >  1:1257334/1‑100 (MQ=255)
gAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGAc                                                                                                     >  2:2627082/1‑100 (MQ=255)
gAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGAc                                                                                                     >  2:2037697/1‑100 (MQ=255)
   ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCa                                                                                                  >  1:3388752/1‑100 (MQ=255)
     cgcgACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAAc                                                                                                <  2:1156476/100‑1 (MQ=255)
      gcgACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAAc                                                                                                <  2:2165915/99‑1 (MQ=255)
        gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACaga                                                                                             >  1:764718/1‑100 (MQ=255)
          ccAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGt                                                                                           >  2:2942926/1‑100 (MQ=255)
           cAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTc                                                                                          >  2:3258103/1‑100 (MQ=255)
            aGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGGGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCt                                                                                         >  1:1877026/1‑100 (MQ=255)
            aGAAATAATGCGATATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCt                                                                                         >  2:2228600/1‑100 (MQ=255)
             gAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCtt                                                                                        <  1:1659359/100‑1 (MQ=255)
              aaaTAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTa                                                                                       <  2:224738/100‑1 (MQ=255)
               aataatGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTAc                                                                                      >  2:2396847/1‑100 (MQ=255)
               aataatGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTAc                                                                                      >  1:3228719/1‑100 (MQ=255)
                 taatGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGc                                                                                    >  1:1917304/1‑100 (MQ=255)
                    tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAgg                                                                                 >  2:1815522/1‑100 (MQ=255)
                    tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAgg                                                                                 <  1:2755071/100‑1 (MQ=255)
                     gCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAggg                                                                                <  2:565618/100‑1 (MQ=255)
                       ggTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCa                                                                              >  1:2726348/1‑100 (MQ=255)
                       ggTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCa                                                                              <  2:2592696/100‑1 (MQ=255)
                             aCTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTGTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTa                                                                        >  1:3153656/1‑100 (MQ=255)
                               tGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGCCGCAACAGAGTCTTACGCAGGGCATTTTTACg                                                                      <  1:2295900/100‑1 (MQ=255)
                               tGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACg                                                                      <  2:911498/100‑1 (MQ=255)
                                gCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACgg                                                                     >  2:1613664/1‑100 (MQ=255)
                                 cccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGa                                                                    <  1:1590672/100‑1 (MQ=255)
                                  ccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGAt                                                                   >  2:103744/1‑100 (MQ=255)
                                  ccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGAt                                                                   >  1:544052/1‑100 (MQ=255)
                                       gagCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGa                                                              >  1:1152395/1‑100 (MQ=255)
                                         gCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTAAGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaa                                                             >  1:934871/1‑99 (MQ=255)
                                           aGTTATTACTGGAAGTGTTATTTCGTAAAGGCGATAAGGTCGTTTTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtt                                                          <  1:542754/100‑1 (MQ=255)
                                            gTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                         >  2:1492506/1‑100 (MQ=255)
                                            gTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                         >  2:758256/1‑100 (MQ=255)
                                              tattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa                                                       >  1:2020800/1‑100 (MQ=255)
                                              tattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa                                                       >  1:860739/1‑100 (MQ=255)
                                              tattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa                                                       >  2:1389577/1‑100 (MQ=255)
                                              tattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa                                                       >  2:153411/1‑100 (MQ=255)
                                              tattaCTCGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGACTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTa                                                       <  1:1812695/100‑1 (MQ=255)
                                               attaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTAt                                                      >  1:1593511/1‑100 (MQ=255)
                                                 taCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCt                                                    <  2:1906404/100‑1 (MQ=255)
                                                   cTGGAAGCGTTGTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                  >  1:1030526/1‑100 (MQ=255)
                                                   cTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTg                                                   >  2:1793989/1‑99 (MQ=255)
                                                   cTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                  >  1:198745/1‑100 (MQ=255)
                                                    tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGGTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGcc                                                 >  1:3047618/1‑100 (MQ=255)
                                                     ggAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg                                                >  2:3077696/1‑100 (MQ=255)
                                                     ggAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg                                                >  1:1347565/1‑100 (MQ=255)
                                                     ggAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATATGCcg                                                >  1:2757665/1‑100 (MQ=255)
                                                      gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc                                               <  1:3069556/100‑1 (MQ=255)
                                                      gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc                                               <  1:796594/100‑1 (MQ=255)
                                                      gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc                                               <  2:1693248/100‑1 (MQ=255)
                                                       aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGCCGCAACAGAGTCTTACGCAGGGCATTGTTACGGATGCCGAAATTTTATCTTCcgcg                                              >  1:817839/1‑100 (MQ=255)
                                                         gCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTa                                            <  1:1140100/100‑1 (MQ=255)
                                                           gTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaa                                          <  2:1939417/100‑1 (MQ=255)
                                                            ttATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaaa                                         >  2:2119498/1‑100 (MQ=255)
                                                              aTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAg                                       >  1:1587746/1‑100 (MQ=255)
                                                              aTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAg                                       <  2:1578382/100‑1 (MQ=255)
                                                              aTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAg                                       <  2:544055/100‑1 (MQ=255)
                                                                ttCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTc                                     <  2:764721/100‑1 (MQ=255)
                                                                ttCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTc                                     >  2:562048/1‑100 (MQ=255)
                                                                  cGTAAAGGCGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCtt                                   >  1:230769/1‑100 (MQ=255)
                                                                  cGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCtt                                   >  2:1217637/1‑100 (MQ=255)
                                                                   gTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTc                                  >  2:1015849/1‑100 (MQ=255)
                                                                    tAAAGGCGATAAGGTCGTTCTTACGGGTAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATATGCCGCGTAAAAAGTCTTc                                  <  1:640211/99‑1 (MQ=255)
                                                                       atgccaTAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGttt                              <  2:1673420/95‑1 (MQ=255)
                                                                       aGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGttt                              <  2:1967713/100‑1 (MQ=255)
                                                                        ggCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGtttt                             >  1:2595107/1‑100 (MQ=255)
                                                                         gCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTa                            <  2:1985085/100‑1 (MQ=255)
                                                                          cGATAAGGTCGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                           <  2:1822746/100‑1 (MQ=255)
                                                                          cGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                           >  2:3328068/1‑100 (MQ=255)
                                                                          cGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                           >  2:2545154/1‑100 (MQ=255)
                                                                          cGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                           >  2:1743667/1‑100 (MQ=255)
                                                                           gATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAcc                          <  1:2995731/100‑1 (MQ=255)
                                                                           gATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAcc                          <  2:276598/100‑1 (MQ=255)
                                                                            aTAAGGCCGTTCGTACGGGGCGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATATGCCGCGTAAAAAGTCTTCGTTTTa                            <  1:1855197/97‑1 (MQ=255)
                                                                             tAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCgg                        <  1:2183082/100‑1 (MQ=255)
                                                                                ggTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt                     <  2:325689/100‑1 (MQ=255)
                                                                                ggTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt                     <  2:3384639/100‑1 (MQ=255)
                                                                                ggTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCt                     >  2:541603/1‑100 (MQ=255)
                                                                                 gTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTg                    >  2:1730407/1‑100 (MQ=255)
                                                                                 gTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTg                    <  2:64685/100‑1 (MQ=255)
                                                                                   cGTTCTTACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGAt                  <  1:443008/100‑1 (MQ=255)
                                                                                   cGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGAt                  <  1:3258075/100‑1 (MQ=255)
                                                                                     ttCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATAc                <  1:2125520/100‑1 (MQ=255)
                                                                                     ttCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATAc                <  1:1571345/100‑1 (MQ=255)
                                                                                      tCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACt              <  2:1593518/100‑1 (MQ=255)
                                                                                         tACGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACtttt           >  2:2906153/1‑100 (MQ=255)
                                                                                          aCGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACttttt          <  2:2024339/100‑1 (MQ=255)
                                                                                          aCGGGGAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACttttt          >  2:1709453/1‑100 (MQ=255)
                                                                                          aCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACttttt          <  2:1347571/100‑1 (MQ=255)
                                                                                          aCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACttttt          <  1:758253/100‑1 (MQ=255)
                                                                                           cGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACtttttt         >  2:653671/1‑100 (MQ=255)
                                                                                           cGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACtttttt         >  1:2764283/1‑100 (MQ=255)
                                                                                            ggggAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTg        <  1:762238/100‑1 (MQ=255)
                                                                                             gggAGATGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGc       >  1:803958/1‑100 (MQ=255)
                                                                                             gggAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGc       <  2:522636/100‑1 (MQ=255)
                                                                                             gggAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGc       <  2:3228747/100‑1 (MQ=255)
                                                                                               gagaCGCAACAGAGTATTACGCAGAGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTg        >  1:1527412/1‑97 (MQ=255)
                                                                                                 gaCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTTGCAAt    >  1:2405019/1‑100 (MQ=255)
                                                                                                  aCGCAACAGAGTCTTACGCAGGGCATTTTTGCGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCATCGTATTACAGGAAAGATACTTTTTTGCaa     >  2:1005999/1‑97 (MQ=255)
                                                                                                  aCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATgg  >  1:1407120/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGG  >  NC_000913/1325751‑1325948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: