Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,033 A→G 16.0% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0330AG16.0% 237.2 / 38.8 107L105P (CTG→CCG) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base A (50/39);  new base G (11/6);  total (62/45)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

Only 100 of 108 total aligned reads displayed.

CGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTC  >  NC_000913/1343935‑1344131
                                                                                                  |                                                                                                  
cGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATTTAGCTGCTGACCGTTATGATAGTGACATTTTTCTTCTGCTCGCCCag                                                                                                   <  1:278751/100‑1 (MQ=255)
       gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                            >  2:1496981/1‑100 (MQ=255)
          cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                         >  1:2244390/1‑100 (MQ=255)
          cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                         >  1:3139146/1‑100 (MQ=255)
           aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                        >  2:2313229/1‑100 (MQ=255)
            aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                       >  2:2217155/1‑100 (MQ=255)
              aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgca                                                                                     <  2:1954993/100‑3 (MQ=255)
              aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgca                                                                                     <  2:1005070/100‑3 (MQ=38)
               tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                    <  1:216872/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAGCAgaa                                                                                  <  2:663875/100‑3 (MQ=38)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  2:1300034/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  2:9458/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  1:2317296/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  1:3332917/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  1:3333317/100‑1 (MQ=255)
                 aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                  <  1:988800/100‑1 (MQ=255)
                  cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACCTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgcgc                                                                                 >  1:751400/1‑100 (MQ=255)
                  cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                 >  1:487650/1‑100 (MQ=255)
                  cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                 >  2:2342123/1‑100 (MQ=255)
                  cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgcagaag                                                                                 <  2:2978631/100‑7 (MQ=37)
                   ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCa                                                                                >  2:1513944/1‑100 (MQ=255)
                   ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCa                                                                                <  1:1513937/100‑1 (MQ=255)
                     cacaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                              >  1:1139972/1‑100 (MQ=255)
                      acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg                                                                             >  2:162898/1‑100 (MQ=255)
                      acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg                                                                             >  2:1840304/1‑100 (MQ=255)
                       caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCCGAGTCCGGGCGAGTAGGGCATtgc                                                                            >  1:631203/1‑100 (MQ=255)
                       caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                            <  1:246210/100‑1 (MQ=255)
                       caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                            <  1:788560/100‑1 (MQ=255)
                              cgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGTTGCTGcc                                                                     <  2:2011579/100‑1 (MQ=255)
                              cgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGcc                                                                     <  1:2988731/100‑1 (MQ=255)
                                   ttCAGCAAATGCGCGATGTAGCTGCTGGGCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                <  2:2383452/100‑1 (MQ=255)
                                   ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                >  2:2846736/1‑100 (MQ=255)
                                    tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                               <  2:1003605/100‑1 (MQ=255)
                                     cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGCGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                               <  2:2020572/99‑1 (MQ=255)
                                      aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTCGGGCATTGCTGCTGCCATTTTCGa                                                             >  2:2130299/1‑100 (MQ=255)
                                        cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                           <  2:1119217/100‑1 (MQ=255)
                                        cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                           >  2:1481027/1‑100 (MQ=255)
                                        cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                           >  1:1801692/1‑100 (MQ=255)
                                        cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGGCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                           <  1:1810779/100‑1 (MQ=255)
                                         aaaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaa                                                          <  1:2716684/100‑1 (MQ=255)
                                          aaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                         >  1:2655555/1‑100 (MQ=255)
                                           aTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                        <  2:3342389/100‑1 (MQ=255)
                                             gcgcGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAt                                                      >  1:3062801/1‑100 (MQ=255)
                                              cgcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATg                                                     >  1:2244212/1‑100 (MQ=255)
                                               gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                    >  1:1500232/1‑100 (MQ=255)
                                               gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                    >  2:2587588/1‑100 (MQ=255)
                                                  aTGTAGCTGCTGACCGTGATGATAGTAACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGttt                                                 <  1:1955026/100‑1 (MQ=255)
                                                     tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGc                                              >  1:1044677/1‑100 (MQ=255)
                                                      ggctgctGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                             <  1:771361/99‑1 (MQ=255)
                                                        ctgctgACCGTGATGATAGTGACATTTTTTTTGTGCTCGCCGAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAGGATGGTTTCGCTCg                                           >  1:3218247/1‑100 (MQ=255)
                                                         tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                          >  1:2799037/1‑100 (MQ=255)
                                                          gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                         <  1:1089601/100‑1 (MQ=255)
                                                             gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa                                      >  2:3113167/1‑100 (MQ=255)
                                                             gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa                                      >  1:797983/1‑100 (MQ=255)
                                                               ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                    <  1:1594812/100‑1 (MQ=255)
                                                                cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                   <  2:3154511/100‑1 (MQ=255)
                                                                cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                   <  1:1199852/100‑1 (MQ=255)
                                                                    atgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTCCTTCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc                                >  1:1776662/1‑99 (MQ=255)
                                                                    aggatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCg                               <  1:2042645/98‑1 (MQ=255)
                                                                     tgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGa                              >  1:1489370/1‑100 (MQ=255)
                                                                        tAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTGGGGCATTGCTGCTGCCATTGTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgc                           >  1:1843404/1‑100 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                          >  2:2572787/1‑99 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                          <  1:3310552/99‑1 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                         >  2:614660/1‑100 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                         >  1:2667802/1‑100 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                         >  1:1025269/1‑100 (MQ=255)
                                                                          gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                         >  2:2133482/1‑100 (MQ=255)
                                                                           tGACGTTTTTCTTCGGCTCGCCCAGAGTCGGGGCGACTGGGGCATTGCTTCTGCCATTTTCGATCAAGATGGTGTCGCCCGGGTGAACCAGCGACGCGGc                        <  1:3362520/100‑1 (MQ=255)
                                                                           tGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGc                        <  1:1826359/100‑1 (MQ=255)
                                                                           tGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGc                        <  1:2572767/100‑1 (MQ=255)
                                                                             aCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaa                      <  2:336400/100‑1 (MQ=255)
                                                                                  aatgccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg                 >  2:1242093/10‑100 (MQ=25)
                                                                                   ttcttcTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCgg                >  1:2399500/1‑100 (MQ=255)
                                                                                    tgccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc               >  1:2793444/8‑100 (MQ=38)
                                                                                    tgccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc               >  1:2792901/8‑100 (MQ=38)
                                                                                     gccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc               >  1:1525529/7‑99 (MQ=37)
                                                                                     cttctGCTCGCCCAGAGTCCGGGCGCGTAGGGCATTGCTGCTGCCATTTTCGATCAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg                 >  1:343863/1‑97 (MQ=255)
                                                                                     cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg              <  2:1141625/100‑1 (MQ=255)
                                                                                     cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg              >  1:2607708/1‑100 (MQ=255)
                                                                                     cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg              >  2:1402939/1‑100 (MQ=255)
                                                                                     cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg              >  1:710049/1‑100 (MQ=255)
                                                                                      ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa             >  2:2363240/1‑100 (MQ=255)
                                                                                      ccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCCGCGACGCGGCAAACTCGGCGa             >  2:1598048/6‑100 (MQ=38)
                                                                                       tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg            >  1:1728867/1‑100 (MQ=255)
                                                                                       tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg            <  1:1863336/100‑1 (MQ=255)
                                                                                       cctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg            >  2:3362045/5‑100 (MQ=38)
                                                                                        ctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt           >  1:422542/4‑100 (MQ=38)
                                                                                        ctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt           <  1:1026597/100‑1 (MQ=255)
                                                                                        ctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt           >  2:833933/1‑100 (MQ=255)
                                                                                         tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt          >  2:1410067/3‑100 (MQ=255)
                                                                                         tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCTTTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg            <  1:2166734/98‑1 (MQ=255)
                                                                                          gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc         >  2:3320064/1‑100 (MQ=255)
                                                                                          gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc         >  1:2652896/1‑100 (MQ=255)
                                                                                           cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc         >  1:665253/1‑99 (MQ=255)
                                                                                           cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa        >  2:2140446/1‑100 (MQ=255)
                                                                                             cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGCACCAGCGACGCGGCAAACTCGGCGAGTTCACg      >  1:3342139/1‑100 (MQ=255)
                                                                                             cGCCCAGAGTACGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAGGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg      >  1:815955/1‑100 (MQ=255)
                                                                                              gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc     <  2:1801703/100‑1 (MQ=255)
                                                                                               cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt    <  1:2979251/100‑1 (MQ=255)
                                                                                                 cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc  >  1:771178/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
CGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTC  >  NC_000913/1343935‑1344131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: