Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,493,774 A→G 62.1% L435L (TTA→TTG trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,7740AG62.1% 105.4 / 125.7 124L435L (TTA→TTGtrgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (14/33);  new base G (34/43);  total (48/76)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 124 total aligned reads displayed.

GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGC  >  NC_000913/1493677‑1493872
                                                                                                 |                                                                                                  
gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGAt                                                                                                  <  1:2534849/100‑1 (MQ=255)
 aTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGAtt                                                                                                 >  2:823197/1‑100 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                                <  1:3305531/100‑1 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                                >  2:2088482/1‑100 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                                <  1:702346/100‑1 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAAtt                                                                                                 >  1:3288576/1‑99 (MQ=255)
   ttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGa                                                                                               <  2:2436801/100‑1 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCGGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAcc                                                                                             >  2:1233952/1‑100 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAcc                                                                                             <  2:318878/100‑1 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGAcc                                                                                             >  1:576093/1‑100 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgg                                                                                          >  1:95234/1‑100 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgg                                                                                          <  2:2646812/100‑1 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTgg                                                                                          <  1:2988089/100‑1 (MQ=255)
        gTTGTCGCCAGCGAAATACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgg                                                                                          <  2:3331998/100‑1 (MQ=255)
          tGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgggg                                                                                        <  2:213095/100‑1 (MQ=255)
            tCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTCAATCAACCTgactga                                                                                      <  2:2068237/100‑6 (MQ=255)
             cGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCg                                                                                     >  1:3054540/1‑100 (MQ=255)
             cGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCg                                                                                     >  2:1104805/1‑100 (MQ=255)
             cGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCg                                                                                     <  2:2333905/100‑1 (MQ=255)
              gCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGt                                                                                       >  1:2729085/1‑97 (MQ=255)
              gCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCgg                                                                                    >  1:1997373/1‑100 (MQ=255)
              gCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCgg                                                                                    >  2:1772994/1‑100 (MQ=255)
                cAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTCAATTAACCTgactgattc                                                                                   <  1:504465/99‑9 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGt                                                                             >  1:773833/1‑100 (MQ=255)
                      aGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTg                                                                            >  2:2621393/1‑100 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTgg                                                                           <  2:2861262/100‑1 (MQ=255)
                        tACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGc                                                                          >  2:735297/1‑100 (MQ=255)
                          cGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCaa                                                                        >  1:1887136/1‑100 (MQ=255)
                          cGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCaa                                                                        <  2:62163/100‑1 (MQ=255)
                            gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACg                                                                      <  1:3045102/100‑1 (MQ=255)
                             acacTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACgg                                                                     >  2:1120480/1‑100 (MQ=255)
                              cacTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGGATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGc                                                                    <  1:1311536/100‑1 (MQ=255)
                               acTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGcc                                                                   <  2:62446/100‑1 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                                  >  1:1785022/1‑100 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                                  <  1:2529718/100‑1 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                                  >  2:2920874/1‑100 (MQ=255)
                                 tCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCgg                                                                 <  1:3149106/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                <  2:3123818/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                <  2:1242146/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                >  1:3361627/1‑100 (MQ=255)
                                    cAAGTCGCAGGGCTCAGGCGGCGAAAGAGATGGAAGGCTTGATCAGTGAATGAGTCAGGTTGATTGACATGGGGTCGGATGAGGTGGCAGCGGCCgg                                                                 <  1:451976/97‑1 (MQ=255)
                                       gTCGCAGTGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAc                                                           <  1:2190516/100‑1 (MQ=255)
                                         cGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCa                                                         <  2:827698/100‑1 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                        >  2:111150/1‑100 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                        <  2:1061015/100‑1 (MQ=255)
                                           cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                       <  2:1612682/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                    <  2:2439851/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                    <  2:2442918/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                    <  1:2454471/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                    <  2:3260067/100‑1 (MQ=255)
                                                cTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCAc                                                  <  2:1735877/100‑1 (MQ=255)
                                                 tCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACt                                                 <  2:1195091/100‑1 (MQ=255)
                                                   aGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAt                                               <  2:1161506/100‑1 (MQ=255)
                                                   aGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAt                                               <  2:2786480/100‑1 (MQ=255)
                                                    ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt                                              >  1:2859484/1‑100 (MQ=255)
                                                     gcggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttg                                             >  1:2654589/1‑100 (MQ=255)
                                                       ggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgtt                                           >  2:2275555/1‑100 (MQ=255)
                                                       ggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgtt                                           <  1:2522453/100‑1 (MQ=255)
                                                        gcgAAAGAGATTGAAGGCTTGATCAGTGAATTAGTCACGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                          <  1:2281255/100‑1 (MQ=255)
                                                        gcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                          <  1:2860033/100‑1 (MQ=255)
                                                          gAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCAGGCGTCGGACCAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt                                              <  1:2874349/94‑1 (MQ=255)
                                                            aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGc                                      >  1:3004579/1‑100 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGcc                                     <  1:186655/99‑1 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCg                                    <  1:2833480/100‑1 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCg                                    <  2:2634013/100‑1 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCg                                    <  2:2265834/100‑1 (MQ=255)
                                                                gaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTc                                  >  2:2235503/1‑100 (MQ=255)
                                                                 aTTGAAGGCTTGATCGGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACCGCCGGGAAAACCATGGGCACTATTGTTGATGCCg                                    >  1:2284947/1‑97 (MQ=255)
                                                                  ttGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                <  2:668952/100‑1 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGa                              >  1:3198581/1‑100 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGa                              >  1:40545/1‑100 (MQ=255)
                                                                      aGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAg                             >  1:2204218/1‑99 (MQ=255)
                                                                      aGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGGCGCGAg                             <  1:1041909/99‑1 (MQ=255)
                                                                         cTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                         >  1:1254193/1‑100 (MQ=255)
                                                                         cTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                         <  2:1166353/100‑1 (MQ=255)
                                                                         cTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                         <  2:576096/100‑1 (MQ=255)
                                                                           tGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcac                       <  1:1712640/100‑1 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                      <  2:2880598/100‑1 (MQ=255)
                                                                             aTCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac                     <  2:2336005/100‑1 (MQ=255)
                                                                               cAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACat                   <  2:1582520/100‑1 (MQ=255)
                                                                               cAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGGGTCACACat                   >  2:1332439/1‑100 (MQ=255)
                                                                                aGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata                  >  2:138234/1‑100 (MQ=255)
                                                                                  tGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATc                >  2:1131781/1‑100 (MQ=255)
                                                                                   gAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCa               <  2:191816/100‑1 (MQ=255)
                                                                                   gAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCa               >  2:1938773/1‑100 (MQ=255)
                                                                                     aTCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATg             <  2:2564182/100‑1 (MQ=255)
                                                                                       cagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGACGTCGCGAGTGTCACACATATCATGCa           >  1:706935/1‑100 (MQ=255)
                                                                                        agtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg          <  1:1246829/100‑1 (MQ=255)
                                                                                        agtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg          <  2:618665/100‑1 (MQ=255)
                                                                                        agtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg          >  2:1488349/1‑100 (MQ=255)
                                                                                        agtcagGTGAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACAAATAGCATGCAg          <  1:1772983/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg         <  2:1254197/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg         <  1:2377554/100‑1 (MQ=255)
                                                                                          tcagGTTAAGTGACCTGGGGTCGGATGAGGTGGCACCGGCCGGGAAAACCATTAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa           <  1:116721/97‑1 (MQ=255)
                                                                                           cagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGa        <  1:2466307/99‑1 (MQ=255)
                                                                                            agGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGaaa      <  1:2788504/100‑1 (MQ=255)
                                                                                             ggTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt     >  1:1689901/1‑100 (MQ=255)
                                                                                             ggTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt     >  1:3252183/1‑100 (MQ=255)
                                                                                               ttAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg   <  1:1826681/100‑1 (MQ=255)
                                                                                                tAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcgc  <  1:703087/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGC  >  NC_000913/1493677‑1493872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: