Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,324 A→G 55.1% S116P (TCT→CCT)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,3240AG55.1% 33.7 / 127.8 103S116P (TCT→CCT) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base G (26/30);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 105 total aligned reads displayed.

CTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAG  >  NC_000913/1555225‑1555422
                                                                                                   |                                                                                                  
cTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGa                                                                                                    >  1:2763661/1‑100 (MQ=255)
cTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGa                                                                                                    >  1:3230481/1‑100 (MQ=255)
     atatTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAAc                                                                                               >  2:378941/1‑100 (MQ=255)
     atatTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAAc                                                                                               <  2:1258290/100‑1 (MQ=255)
       attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                             <  2:3278171/100‑1 (MQ=255)
       attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGc                                                                                             >  2:945311/1‑100 (MQ=255)
        ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                             >  2:3322136/1‑99 (MQ=255)
        ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCt                                                                                            <  1:2624236/100‑1 (MQ=255)
         tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTc                                                                                           >  2:951548/1‑100 (MQ=255)
         tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTc                                                                                           <  1:2490411/100‑1 (MQ=255)
          atGGTTCGGCACGTTTTGCAGAATATCGTCCATAGTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTca                                                                                          <  1:2057510/100‑1 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg                                                                                       >  1:61430/1‑100 (MQ=255)
               tCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGc                                                                                     >  2:1259311/1‑100 (MQ=255)
                 ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                   <  2:2355046/100‑1 (MQ=255)
                  gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc                                                                                  <  1:194387/100‑1 (MQ=255)
                  gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGGTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGAGCTCAGGAAAACGCTCACAGGc                                                                                     >  1:1124757/1‑97 (MQ=255)
                      gTTTTGCAGAATATCGTCCATAGTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGTAAAACGCTCACAGGGTGAGCCg                                                                              <  1:1105697/100‑1 (MQ=255)
                       ttttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGa                                                                             >  1:1294125/1‑100 (MQ=255)
                         ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACg                                                                           >  2:2102042/1‑100 (MQ=255)
                          tGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACgg                                                                          >  1:546196/1‑100 (MQ=255)
                           gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGt                                                                         >  2:2641955/1‑100 (MQ=255)
                           gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGt                                                                         >  2:57624/1‑100 (MQ=255)
                           gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGt                                                                         <  2:2961745/100‑1 (MQ=255)
                            cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGtt                                                                        <  1:1274223/100‑1 (MQ=255)
                            cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGtt                                                                        <  2:3158753/100‑1 (MQ=255)
                            cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGtt                                                                        >  1:1580325/1‑100 (MQ=255)
                              gAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTgg                                                                      <  2:2722420/100‑1 (MQ=255)
                                atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTgggg                                                                    <  1:921015/100‑1 (MQ=255)
                                atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTgggg                                                                    <  1:3287035/100‑1 (MQ=255)
                                  atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta                                                                  >  1:266980/1‑100 (MQ=255)
                                  atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta                                                                  <  2:2849773/100‑1 (MQ=255)
                                  atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta                                                                  <  2:3370538/100‑1 (MQ=255)
                                     gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                                >  1:1529917/1‑99 (MQ=255)
                                     gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa                                                               <  2:882316/100‑1 (MQ=255)
                                     gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa                                                               >  2:340206/1‑100 (MQ=255)
                                     gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa                                                               >  2:262476/1‑100 (MQ=255)
                                      tCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaa                                                              >  2:1272388/1‑100 (MQ=255)
                                      tCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaa                                                              <  2:136121/100‑1 (MQ=255)
                                       ccATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaat                                                             <  2:575054/100‑1 (MQ=255)
                                        cATATTGTGCCGGTTCTGGTAATAGATAAACACGCCGCGTTAACCGCGGTAGATCTCAGAAAAACTCTCATAGGCTTTGCCGACGTTTGGGGTatacat                                                             <  1:2489161/99‑1 (MQ=255)
                                        cATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaata                                                            <  1:591389/100‑1 (MQ=255)
                                           atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa                                                         >  1:2816031/1‑100 (MQ=255)
                                           atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATTAATAACa                                                         <  2:1770776/100‑1 (MQ=255)
                                           atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa                                                         <  2:1028118/100‑1 (MQ=255)
                                            ttGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAg                                                        <  1:575848/100‑1 (MQ=255)
                                            ttGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAg                                                        <  1:2114515/100‑1 (MQ=255)
                                              gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                      >  2:2329230/1‑100 (MQ=255)
                                              gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                      <  2:2640741/100‑1 (MQ=255)
                                              gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                      >  2:2361829/1‑100 (MQ=255)
                                               tgCCGGTTCTGGTAAGAGATCAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGca                                                     >  1:2073690/1‑100 (MQ=255)
                                               tgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGca                                                     <  2:1406053/100‑1 (MQ=255)
                                                   ggTTCTGGTAAGTGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatcat                                                 >  1:1521208/1‑100 (MQ=255)
                                                   ggTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatcat                                                 >  2:1183118/1‑100 (MQ=255)
                                                     ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct                                               <  2:2540510/100‑1 (MQ=255)
                                                       cTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctca                                             <  2:1027124/100‑1 (MQ=255)
                                                        tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                            <  2:1203150/100‑1 (MQ=255)
                                                         ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCGGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                           >  2:145315/1‑100 (MQ=255)
                                                         ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                           >  2:2966731/1‑100 (MQ=255)
                                                          gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACGGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                          >  2:2447301/1‑100 (MQ=255)
                                                           tAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCaa                                         >  2:479183/1‑100 (MQ=255)
                                                            aaGAGAGAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg                                        <  1:1241814/100‑1 (MQ=255)
                                                             agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga                                       <  1:2640183/100‑1 (MQ=255)
                                                              gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                      >  2:2449733/1‑100 (MQ=255)
                                                                gaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatga                                    >  1:1725027/1‑100 (MQ=255)
                                                                 aTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatgat                                   <  2:242544/100‑1 (MQ=255)
                                                                 aTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatgat                                   <  2:22551/100‑1 (MQ=255)
                                                                  tAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                  >  2:1841554/1‑100 (MQ=255)
                                                                   aaaCACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg                                 <  1:2511823/100‑1 (MQ=255)
                                                                   aaaCACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg                                 <  2:679749/100‑1 (MQ=255)
                                                                    aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGt                                >  2:730813/1‑100 (MQ=255)
                                                                      cacGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGttt                              <  1:1103453/100‑1 (MQ=255)
                                                                         gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                           >  1:926945/1‑100 (MQ=255)
                                                                         gccgcGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                           <  1:329673/100‑1 (MQ=255)
                                                                         gccgcGCGAACGGCGGTAGATCTCAGGCAAACGCGCACAGGCTGCGCCGACGGGTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                           <  1:1881664/100‑1 (MQ=255)
                                                                          ccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCa                          <  1:1405651/100‑1 (MQ=255)
                                                                           cgcgTGAACGGCGGTAGATCTCAGGAAAACGCTCACGGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAg                         <  1:2447282/100‑1 (MQ=255)
                                                                            gcgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGa                        <  2:1043704/100‑1 (MQ=255)
                                                                            gcgTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGa                        >  2:2174135/1‑100 (MQ=255)
                                                                               tGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACgg                     >  1:314369/1‑100 (MQ=255)
                                                                               tGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACgg                     <  2:159829/100‑1 (MQ=255)
                                                                                gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGt                    <  1:2204692/100‑1 (MQ=255)
                                                                                gAAAGGCGGTAAATCACAAAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAg                         <  1:324516/95‑1 (MQ=255)
                                                                                 aaCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCGGACGGTa                   <  2:1461691/100‑1 (MQ=255)
                                                                                  aCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTag                  >  1:2959754/1‑100 (MQ=255)
                                                                                     gcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag               >  1:3351437/1‑100 (MQ=255)
                                                                                      cggTAGATCTCAGGACAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag               >  1:989951/1‑99 (MQ=255)
                                                                                       ggTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAagag             >  2:682826/1‑100 (MQ=255)
                                                                                         tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg           <  1:1517284/100‑1 (MQ=255)
                                                                                         tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg           >  1:1781442/1‑100 (MQ=255)
                                                                                         tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg           <  1:968790/100‑1 (MQ=255)
                                                                                         tAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg           <  1:645820/100‑1 (MQ=255)
                                                                                            aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGttt        >  2:3213974/1‑100 (MQ=255)
                                                                                              ctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg      <  2:2460390/100‑1 (MQ=255)
                                                                                              ctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg      <  2:1089068/100‑1 (MQ=255)
                                                                                              ctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg      >  2:1976029/1‑100 (MQ=255)
                                                                                              ctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg      >  1:1430453/1‑100 (MQ=255)
                                                                                              ctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg      >  2:3014339/1‑100 (MQ=255)
                                                                                               tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTTGGGTATAAATAACAGGCATCATCTCATCAAGATTATTGTTTACCAGACGGTAGAAGAGGGTTTCGt     <  1:2599957/100‑1 (MQ=255)
                                                                                               tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt     <  1:2867423/100‑1 (MQ=255)
                                                                                                cAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt    >  2:2238974/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAG  >  NC_000913/1555225‑1555422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: