| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,650,904 | T→C | 51.1% | pseudogene (60/693 nt) | insD1 → | IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,650,904 | 0 | T | C | 51.1% | ‑1.7 / 60.7 | 49 | pseudogene (60/693 nt) | insD1 | IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related |
| Reads supporting (aligned to +/- strand): ref base T (15/9); new base C (12/13); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTG > NC_000913/1650808‑1650941 | aGCAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGtt < 2:3323625/100‑1 (MQ=255) gACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTAcc > 1:2481731/1‑100 (MQ=255) gACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTAcc > 1:58245/1‑100 (MQ=255) gACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTAcc < 2:216898/100‑1 (MQ=255) cATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAATCGTGTTTACCgn < 1:2857810/100‑2 (MQ=255) cATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCgg > 2:129205/1‑100 (MQ=255) tGGAAAACACTGGATGGCGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGAt > 1:934425/1‑100 (MQ=255) ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc < 2:1677396/100‑1 (MQ=255) ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc > 2:1325634/1‑100 (MQ=255) ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc < 2:1119901/100‑1 (MQ=255) ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc > 1:8651/1‑100 (MQ=255) gAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGCTCAATGCCAAACGTGTTTACCGGATc > 2:1621792/1‑99 (MQ=255) gAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCa > 2:989732/1‑100 (MQ=255) gAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCa < 1:2648291/100‑1 (MQ=255) gAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCa > 2:1947627/1‑100 (MQ=255) aaaCACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATg < 1:2201235/100‑1 (MQ=255) ggATGACGAACTGGCCTATGGGCGCTGCTTCGCAGAGAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGa > 1:2368562/1‑100 (MQ=255) ggATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGa > 2:2118609/1‑100 (MQ=255) ggATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGa > 1:2755615/1‑100 (MQ=255) ggATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGa > 1:210764/1‑100 (MQ=255) ggATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGa > 2:2825739/1‑100 (MQ=255) gATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGaa < 2:1223116/100‑1 (MQ=255) aTGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAAt > 2:2213560/1‑100 (MQ=255) gACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATgc < 1:398504/100‑1 (MQ=255) aCGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGTTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATgcg < 1:321472/100‑1 (MQ=255) aCGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATgcg > 1:2873582/1‑100 (MQ=255) aCGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATgcg > 1:1485900/1‑100 (MQ=255) cGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATgcg > 1:2672022/1‑99 (MQ=40) cGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATgcgc < 1:308429/100‑1 (MQ=255) taaCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCt < 2:671868/99‑1 (MQ=38) gAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCt < 1:2825716/100‑1 (MQ=255) aaCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAGTGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTg > 2:2082340/1‑100 (MQ=255) aaCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCta > 1:671865/1‑99 (MQ=38) aaCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTg < 1:1626602/100‑1 (MQ=255) aCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGt > 2:138149/1‑100 (MQ=38) aCTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGt < 2:2893108/100‑1 (MQ=255) cTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGtt < 2:742747/100‑1 (MQ=35) tGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTg > 2:2248112/1‑100 (MQ=35) tGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTg < 2:1295881/100‑1 (MQ=25) tGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATCCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGt > 1:477919/1‑98 (MQ=255) ggCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGc > 1:1758742/1‑100 (MQ=33) ggCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGc < 2:2988425/100‑1 (MQ=33) gCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCt < 2:1578515/100‑1 (MQ=17) gCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCt < 1:2365756/100‑1 (MQ=17) gCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCt > 1:2673596/1‑100 (MQ=17) ccTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCtt > 1:1828608/1‑100 (MQ=14) ccTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCtt > 2:75060/1‑100 (MQ=14) cTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTg < 1:1257537/100‑1 (MQ=11) cTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTg < 2:408889/100‑1 (MQ=11) | AGCAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTG > NC_000913/1650808‑1650941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |