Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	416,389	(C)5→4	coding (564/1203 nt)	sbcD ←	exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	416,385	0	C	.	100.0%	262.2 / NA	56	coding (568/1203 nt)	sbcD	exonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/28);  total (28/28)

TGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGG  >  NC_000913/416288‑416480                                                                                                  |                                                                                                tGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGccc                                                                                               >  2:1051419/1‑100 (MQ=255)  gCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGcccc                                                                                              >  2:1059494/1‑100 (MQ=255)  gCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGcccc                                                                                              <  2:302856/100‑1 (MQ=255)   cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGcccca                                                                                             >  1:377755/1‑99 (MQ=255)   cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGcccca                                                                                             >  2:861169/1‑99 (MQ=255)       aGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGccccacgg                                                                                          >  2:1437598/1‑95 (MQ=255)          gcgATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGt                                                                                     >  1:797446/1‑100 (MQ=255)          gcgATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGt                                                                                     >  2:1243847/1‑100 (MQ=255)          gcgATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGt                                                                                     >  1:1049448/1‑100 (MQ=255)           cgATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGtt                                                                                    <  2:1151925/100‑1 (MQ=255)              tGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTaaa                                                                                 <  2:1003150/100‑1 (MQ=255)              tGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTaaa                                                                                 <  1:465457/100‑1 (MQ=255)               gTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCGCGGTCGTTAAAt                                                                                <  1:1038288/100‑1 (MQ=255)               gTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAAt                                                                                <  2:637440/100‑1 (MQ=255)                 aGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGt                                                                              >  2:161039/1‑100 (MQ=255)                  gTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTc                                                                             >  2:955126/1‑100 (MQ=255)                   tgggCTGGTGGAAAGTTTTGTGCCGGAAGCGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTcc                                                                            <  2:962123/98‑1 (MQ=255)                    cGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTccc                                                                           <  1:1051425/100‑1 (MQ=255)                      gCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑‑CCCACGGTCGTTAAATGTCCCGTg                                                                        <  2:961581/100‑1 (MQ=255)                        tgggggAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTgg                                                                       <  1:1009733/100‑1 (MQ=255)                             gAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCgatg                                                                  >  1:661716/1‑100 (MQ=255)                             gAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCgatg                                                                  >  2:1456905/1‑100 (MQ=255)                              aaaGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCgatga                                                                 >  1:1486977/1‑100 (MQ=255)                              aaaGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATCTACCGTGGCgatga                                                                 >  1:1344591/1‑100 (MQ=255)                               aaGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCgatgat                                                                <  1:518099/100‑1 (MQ=255)                                aGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGatgatg                                                               <  2:1311413/100‑1 (MQ=255)                                  ttttGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATggg                                                             <  1:860252/100‑1 (MQ=255)                                   tttGTGCCGGAAACTCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGc                                                            <  2:1076211/100‑1 (MQ=255)                                     tgtgCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCag                                                          <  2:435364/100‑1 (MQ=255)                                     tgtgCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCag                                                          >  1:1561372/1‑100 (MQ=255)                                     tgtgCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCag                                                          >  1:1077717/1‑100 (MQ=255)                                      gtgCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCaga                                                         <  1:954054/100‑1 (MQ=255)                                        gCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAgg                                                       >  2:619783/1‑100 (MQ=255)                                          cGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGc                                                      <  2:27914/99‑1 (MQ=255)                                          cGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCt                                                     <  1:507171/100‑1 (MQ=255)                                            gAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGa                                                   <  2:567162/100‑1 (MQ=255)                                              aaCGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATc                                                 <  1:1209456/100‑1 (MQ=255)                                               aCGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATcg                                                <  2:90698/100‑1 (MQ=255)                                                   gTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATcgccgc                                            >  2:4695/1‑100 (MQ=255)                                                     ccAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCgcgc                                          >  2:410981/1‑100 (MQ=255)                                                      cAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCa                                         >  2:393177/1‑100 (MQ=255)                                                      cAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCa                                         >  1:638292/1‑100 (MQ=255)                                                           tGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTg                                    >  2:1307625/1‑100 (MQ=255)                                                            gCCAAGATAAATGTCACGCAAGGCGTCACTTTTACTGG‑CGCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGc                                   <  2:323550/100‑1 (MQ=255)                                                             ccAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCa                                  >  2:1298202/1‑100 (MQ=255)                                                              cAATATAAATGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGTGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAg                                 <  2:842798/100‑1 (MQ=255)                                                                  ataAATGTCACGCACGGCGTCACTTTTACTGG‑CCACACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCAt                             >  2:1088896/1‑100 (MQ=255)                                                                    aaaTGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCCGGCATCg                           >  2:509061/1‑100 (MQ=255)                                                                       tGTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCa                        >  1:1505928/1‑100 (MQ=255)                                                                        gTCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCAt                       >  2:1198045/1‑100 (MQ=255)                                                                         tCACGCACGGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATa                      >  1:242108/1‑100 (MQ=255)                                                                          cacgcacgGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAg                     >  1:788188/1‑100 (MQ=255)                                                                              cacgGCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGtgtt                 >  2:1195868/1‑100 (MQ=255)                                                                                  gCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGttgttg             >  2:146987/1‑100 (MQ=255)                                                                                  gCGTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGttgttg             <  1:1187350/100‑1 (MQ=255)                                                                                    gTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGt           <  1:1198050/100‑1 (MQ=255)                                                                                    gTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGt           <  1:861174/100‑1 (MQ=255)                                                                                    gTCACTTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGt           <  1:235329/100‑1 (MQ=255)                                                                                     tCAATTTTACTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGAGGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGt           <  2:952888/99‑1 (MQ=255)                                                                                             aCTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCgg  <  2:1158351/100‑1 (MQ=255)                                                                                             aCTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCgg  >  1:72893/1‑100 (MQ=255)                                                                                             aCTGG‑CCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCgg  >  1:570150/1‑100 (MQ=255)                                                                                                  |                                                                                                TGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGG  >  NC_000913/416288‑416480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑