Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 750,896 Δ1 bp coding (1274/2448 nt) ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913750,8930C.98.2% 245.6 / ‑1.9 54coding (1277/2448 nt)ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (33/20);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCAT  >  NC_000913/750798‑750987
                                                                                               |                                                                                              
tgtgAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGcccgc                                                                                            >  2:1444609/1‑98 (MQ=255)
 gtgAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGcccgct                                                                                           >  2:1738/1‑97 (MQ=255)
   gAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCg                                                                                        <  2:1130325/100‑1 (MQ=255)
      aaTGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTcc                                                                                     <  1:984886/100‑1 (MQ=255)
      aaTGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTcc                                                                                     >  2:165721/1‑100 (MQ=255)
       aTGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCct                                                                                    <  2:865642/100‑1 (MQ=255)
       aTGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCct                                                                                    >  1:1264495/1‑100 (MQ=255)
          aCGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCctgct                                                                                 <  1:1157794/100‑1 (MQ=255)
             aTCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCctgctg                                                                                <  2:1253989/98‑1 (MQ=255)
                 gagaAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATc                                                                          <  2:648356/100‑1 (MQ=255)
                 gagaAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATc                                                                          >  2:1116685/1‑100 (MQ=255)
                           aaGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCTACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATCGCTa                                                                >  2:1533166/1‑100 (MQ=255)
                             gcggcgAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACt                                                              <  2:471301/100‑1 (MQ=255)
                               ggcgAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTgg                                                            <  1:644198/100‑1 (MQ=255)
                                   agcgaAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTCGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCa                                                        <  2:389974/100‑1 (MQ=255)
                                    gcgaAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAg                                                       >  1:19976/1‑100 (MQ=255)
                                     cgaAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAgg                                                      >  1:394246/1‑100 (MQ=255)
                                      gaAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGt                                                     >  1:1091420/1‑100 (MQ=255)
                                      gaAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGt                                                     <  1:1168870/100‑1 (MQ=255)
                                           cGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACAt                                                >  2:1276565/1‑100 (MQ=255)
                                             tGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCa                                              >  1:1205244/1‑100 (MQ=255)
                                             tGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCa                                              >  2:830866/1‑100 (MQ=255)
                                              gCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCaa                                             <  2:1517886/100‑1 (MQ=255)
                                                tGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAg                                           >  2:1148186/1‑100 (MQ=255)
                                                 gTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAgg                                          >  1:253295/1‑100 (MQ=255)
                                                  ttGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTTCTAAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGa                                         >  2:1111727/1‑100 (MQ=255)
                                                    ggtggCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGCCATCAAAGGACa                                       >  2:1464291/1‑100 (MQ=255)
                                                       ggCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTg                                    >  1:744778/1‑100 (MQ=255)
                                                        gCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTg                                    >  2:723157/1‑99 (MQ=255)
                                                         cATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGTCATCAAAGGACAGTgcg                                  >  2:943658/1‑100 (MQ=255)
                                                           ttcACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGcc                                >  1:1071875/3‑100 (MQ=255)
                                                           tCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGcc                                <  2:1005453/100‑1 (MQ=255)
                                                             cACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCaa                              >  2:113318/1‑100 (MQ=255)
                                                              aCTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAg                             <  2:1264490/100‑1 (MQ=255)
                                                                 cttccgatctAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCca                          <  1:976905/91‑1 (MQ=255)
                                                                     tGCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGc                      <  1:1148191/100‑1 (MQ=255)
                                                                      gCTGTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCa                     >  2:985897/1‑100 (MQ=255)
                                                                       cTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCa                     >  1:1467156/1‑100 (MQ=255)
                                                                         gTAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCatat                  >  1:461819/1‑100 (MQ=255)
                                                                          tAATTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATAtt                 >  1:1440263/1‑100 (MQ=255)
                                                                           aaTTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCcaagatcggaag                >  2:976900/1‑90 (MQ=255)
                                                                            aTTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATAtttt               <  1:258933/100‑1 (MQ=255)
                                                                            aTTAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATAtttt               <  2:56560/100‑1 (MQ=255)
                                                                              tAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGc             >  2:1542823/1‑100 (MQ=255)
                                                                              tAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGc             >  2:151974/1‑100 (MQ=255)
                                                                              tAAAACGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGc             >  1:1199249/1‑100 (MQ=255)
                                                                               aaaaCGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGcc            >  1:112225/1‑100 (MQ=255)
                                                                                aaaCGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGccc           <  1:677357/100‑1 (MQ=255)
                                                                                aaaCGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGccc           <  1:950246/100‑1 (MQ=255)
                                                                                   cGGTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGAt        >  1:1438041/1‑100 (MQ=255)
                                                                                    ggTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATa       >  1:508188/1‑100 (MQ=255)
                                                                                    ggTAGCTCAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATa       >  1:827295/1‑100 (MQ=255)
                                                                                     gTAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAc      <  1:1210061/100‑1 (MQ=255)
                                                                                      tAGCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAcc     >  1:1155261/1‑100 (MQ=255)
                                                                                        gCTTAAGCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCa   >  2:1580384/1‑100 (MQ=255)
                                                                                         cTTAAGCCCGCTGGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCAt  >  1:1039361/1‑100 (MQ=255)
                                                                                               |                                                                                              
TGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCAT  >  NC_000913/750798‑750987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: