| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,170,338 | (A)6→5 | coding (37/963 nt) | ldtC ← | L,D‑transpeptidase linking Lpp to murein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,170,333 | 0 | A | . | 98.2% | 246.3 / ‑1.9 | 55 | coding (42/963 nt) | ldtC | L,D‑transpeptidase linking Lpp to murein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (28/26); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTA > NC_000913/1170234‑1170428 | aTGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTa > 2:1434919/1‑100 (MQ=255) tGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaa > 1:1147477/1‑100 (MQ=255) tGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgtt < 2:297450/100‑5 (MQ=255) gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTa < 1:756673/100‑1 (MQ=255) gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTa > 2:212820/1‑100 (MQ=255) gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTa > 1:213953/1‑100 (MQ=255) tCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCa > 1:1479451/1‑100 (MQ=255) tCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCa > 1:241025/1‑100 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAg > 1:414492/1‑100 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAg > 1:543234/1‑100 (MQ=255) gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGc > 2:1551300/1‑100 (MQ=255) gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGc < 1:1434927/100‑1 (MQ=255) gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGc > 2:595475/1‑100 (MQ=255) cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCga > 2:123241/1‑100 (MQ=255) aaCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGCCAGCGGCGAACGT‑AAAAACGTTAGCCAGCgag > 2:297004/1‑100 (MQ=255) caacaccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGaaaa > 2:1174634/7‑100 (MQ=37) ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcgcg > 2:1245556/2‑100 (MQ=255) ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcgcg > 1:1158177/2‑100 (MQ=255) acTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcgcg > 2:1297826/1‑100 (MQ=255) tGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGtt < 2:1486169/100‑1 (MQ=255) gCCCGCTGGCGGCAGCGCCCAGGTGTTGGCTTTTGACGGTACCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGttt < 2:410888/100‑1 (MQ=255) ccGCTGGCGGCAGCGGCCAGCTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcgcg < 2:838228/95‑1 (MQ=255) cGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTg < 2:221411/99‑1 (MQ=255) gCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat > 1:657579/1‑100 (MQ=255) ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcat > 1:1349751/1‑100 (MQ=255) gcggcagcggcaAGGTGTTGGCTTTTGCCGGTAGCGCCATCGGAACGGCAGCGGCGAACGT‑AAAAACGTTAGAAAGATAGAAAAACGCGTTTTGatcatc < 2:1112122/100‑1 (MQ=255) gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc < 2:619971/100‑1 (MQ=255) gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGGTTTGatcatc > 2:646655/1‑100 (MQ=255) gCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAAt > 1:1097166/1‑100 (MQ=255) cAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATcc > 2:404253/1‑100 (MQ=255) aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa < 2:910616/100‑1 (MQ=255) ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat < 1:1551310/100‑1 (MQ=255) ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat < 2:344382/100‑1 (MQ=255) gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 2:1522480/100‑1 (MQ=255) ggCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa > 2:228128/1‑100 (MQ=255) ttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATaa < 2:845354/100‑1 (MQ=255) tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg > 1:400279/1‑100 (MQ=255) tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg < 1:26808/100‑1 (MQ=255) gCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAgg > 1:197623/1‑100 (MQ=255) ccGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGt > 1:1364522/1‑100 (MQ=255) gTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt < 1:462069/100‑1 (MQ=255) agcgccagcgccACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg > 2:998161/1‑100 (MQ=255) agcgccagcgccACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg < 1:50756/100‑1 (MQ=255) cgccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta < 2:1079787/100‑1 (MQ=255) ccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaat > 1:409791/1‑100 (MQ=255) cagcgccaCGGCAGAGGCGAACGT‑AAAAACGTTAGCAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaata < 2:1397781/100‑1 (MQ=255) cgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc > 1:161105/1‑100 (MQ=255) gccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa < 1:595477/100‑1 (MQ=255) gccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTAAAAAAAAAAAAAAAATCCATAATAAATATAAGGTTATTg < 2:576387/91‑1 (MQ=255) ccaCGGCCGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTCTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAg > 2:306277/1‑100 (MQ=255) caCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa < 2:543233/100‑1 (MQ=255) aCGGCAGCGGCGAACGTAAAAAACGTTAGCCAGAGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa < 2:1321315/100‑1 (MQ=255) aGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc < 1:242388/100‑1 (MQ=255) aGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc < 1:123241/100‑1 (MQ=255) cggcgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATACGCCTg > 1:378586/1‑100 (MQ=255) cggcgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTg < 1:825785/100‑1 (MQ=255) ggcgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATACTAAATATAAGGTTATTGTAATAAACAGATAAGCCt < 2:708226/98‑1 (MQ=255) aaCGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTa < 1:1057004/100‑1 (MQ=255) | ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTA > NC_000913/1170234‑1170428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |