Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,518	(C)6→5	intergenic (+360/‑251)	nth → / → dtpA	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,513	0	C	.	100.0%	203.3 / NA	44	intergenic (+355/‑256)	nth/dtpA	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/20);  total (24/20)

GACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712418‑1712606                                                                                                |                                                                                              gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           >  2:257926/1‑100 (MQ=255) gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           <  1:303260/100‑1 (MQ=255) gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccc                                                                                             <  2:1481406/98‑1 (MQ=255)   ncTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctc                                                                                         >  1:1234190/2‑98 (MQ=255)     tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                       >  1:208461/1‑96 (MQ=255)         aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTcagtg                                                                                  >  1:1570032/1‑100 (MQ=255)          aaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTcagtgc                                                                                 <  1:682643/100‑1 (MQ=255)          aaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTcagtgc                                                                                 >  2:633988/1‑100 (MQ=255)                    tGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGaaaaaa                                                                       >  1:875726/1‑100 (MQ=255)                     ggCTATTTGTCAGAAACTGGACTATCTCACTAGTAAAAACGCTCAATAGCAGAACATATCGCCGTTAAGCCCTCA‑CCCCCTCTCAATGCAATGAAAAAAt                                                                      >  2:1377905/1‑100 (MQ=255)                      gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATc                                                                     >  2:685553/1‑100 (MQ=255)                         aTTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGc                                                                  >  1:1021834/1‑100 (MQ=255)                          tttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                 <  1:492540/100‑1 (MQ=255)                           ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCg                                                                <  1:62435/100‑1 (MQ=255)                               cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                            <  2:1372673/100‑1 (MQ=255)                                aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           <  1:725133/100‑1 (MQ=255)                                 gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           >  2:61686/1‑99 (MQ=255)                                   aaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGttt                                                        >  1:373938/1‑100 (MQ=255)                                      tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  1:685555/100‑1 (MQ=255)                                       ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACAGGTCGCCGTTAAGCCATCA‑CCCCGTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  2:101779/99‑1 (MQ=255)                                          cTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaa                                                 >  1:1366017/1‑100 (MQ=255)                                            aTCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaaaa                                               >  1:78564/1‑100 (MQ=255)                                             tctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAt                                              <  2:199561/100‑1 (MQ=255)                                              ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                             >  1:1151153/1‑100 (MQ=255)                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  1:437319/1‑100 (MQ=255)                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           <  1:1336918/100‑1 (MQ=255)                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  2:753831/1‑100 (MQ=255)                                                  cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTATGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         >  2:970953/1‑100 (MQ=255)                                                  cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         >  1:218781/1‑100 (MQ=255)                                                  cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         >  2:527233/1‑100 (MQ=255)                                                      tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                     <  1:753833/100‑1 (MQ=255)                                                      tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                     <  2:1435952/100‑1 (MQ=255)                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTgg                                    <  2:1298103/100‑1 (MQ=255)                                                        aaaaCGCTAAATATCATAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGa                                   <  2:1378234/100‑1 (MQ=255)                                                         aaaCGCTAAATAGCAGAACATATCGCCGTTAAACCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCTGTTACGTTTTTTGAAAAATTTAACGCTGGAt                                  <  1:647608/100‑1 (MQ=255)                                                              ntAAATAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             >  1:949140/2‑100 (MQ=255)                                                                  aTAGCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                         >  1:1058734/1‑100 (MQ=255)                                                                    cgCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTccc                        <  2:958753/98‑1 (MQ=255)                                                                     gCAGAACATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      <  1:633990/100‑1 (MQ=255)                                                                         aaCATATCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATg                  <  1:1170082/100‑1 (MQ=255)                                                                            atatCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGtt               >  2:786353/1‑100 (MQ=255)                                                                            atatCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGt                >  2:280367/1‑99 (MQ=255)                                                                             tatCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTg              <  2:1395457/100‑1 (MQ=255)                                                                             tatCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAAGATAATAGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTg              <  2:287756/100‑1 (MQ=255)                                                                              atCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa             >  1:686220/1‑100 (MQ=255)                                                                              atCGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa             <  1:1454888/100‑1 (MQ=255)                                                                                cGCCGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAt           >  2:234691/1‑100 (MQ=255)                                                                                  ccGTTAAGCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTc         >  2:506102/1‑100 (MQ=255)                                                                                         gCCATCA‑CCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt  >  1:176348/1‑100 (MQ=255)                                                                                                |                                                                                              GACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712418‑1712606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑