Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T95.8% 245.8 / 3.6 71coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base T (25/43);  total (27/44)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAC  >  NC_000913/2469551‑2469740
                                                                                                   |                                                                                            
aTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑t                                                                                            >  2:1113914/1‑100 (MQ=255)
 tGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑tt                                                                                           >  1:1473089/1‑100 (MQ=255)
    ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttt                                                                                        <  1:1156873/100‑1 (MQ=255)
      gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttt                                                                                      >  2:988037/1‑100 (MQ=255)
      gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttt                                                                                      <  1:585720/100‑1 (MQ=255)
       catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑tttttttt                                                                                     >  2:239772/1‑99 (MQ=255)
       catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttt                                                                                      <  2:1456200/99‑1 (MQ=255)
        atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                    <  2:736054/100‑3 (MQ=255)
         tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttcg                                                                                   <  2:1118263/100‑4 (MQ=255)
         tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑ttttttttc                                                                                    >  2:87280/1‑97 (MQ=255)
           atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTTTTTTTTTTTTTAACATACAAAAACAATAAATCTATGCTTTTTCTt‑‑ttttttttcgt                                                                                  >  2:1172328/1‑95 (MQ=255)
            ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                 <  1:734019/100‑1 (MQ=255)
             tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                <  2:1470576/100‑1 (MQ=255)
              cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                               <  1:40298/100‑1 (MQ=255)
              cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                               <  2:745920/100‑1 (MQ=255)
                aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTCGTTGCta                                                                              >  2:895606/1‑100 (MQ=255)
                aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                             <  1:650148/100‑1 (MQ=255)
                  cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                           <  1:216234/100‑1 (MQ=255)
                   aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                          >  1:1151600/1‑100 (MQ=255)
                   aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                          >  2:1148941/1‑100 (MQ=255)
                     ggtcAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                         <  2:329969/96‑1 (MQ=255)
                       gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCt                                                                     <  2:767235/100‑1 (MQ=255)
                        cAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCt                                                                     <  2:1425521/100‑1 (MQ=255)
                           aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                  <  2:1167238/100‑1 (MQ=255)
                           aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                  <  2:1270788/100‑1 (MQ=255)
                               ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTa                                                              >  1:1145586/1‑100 (MQ=255)
                               ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTa                                                              >  2:811495/1‑100 (MQ=255)
                                   aaTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaaa                                                          <  2:1246595/100‑1 (MQ=255)
                                    aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                         <  2:1151595/100‑1 (MQ=255)
                                      tttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                       <  1:1507140/100‑1 (MQ=255)
                                       ttccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCaa                                                      <  2:1473081/100‑1 (MQ=255)
                                        tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAACTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                     <  1:708136/100‑1 (MQ=255)
                                         ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAtt                                                    >  2:349273/1‑100 (MQ=255)
                                          cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                   <  1:634654/100‑1 (MQ=255)
                                          cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                   >  1:1339339/1‑100 (MQ=255)
                                          cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                  >  2:109528/1‑100 (MQ=255)
                                           ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                  >  1:669970/1‑100 (MQ=255)
                                           ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                  >  1:1531707/1‑100 (MQ=255)
                                           ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                  <  1:1435981/100‑1 (MQ=255)
                                             ccATTTTTTGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                <  1:1288094/100‑1 (MQ=255)
                                             ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                <  1:931666/100‑1 (MQ=255)
                                                tttttGGTGTATTCATTAACATGCAAAAACAATAAATCTATTCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTa                                             <  2:318298/100‑1 (MQ=255)
                                                    tGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAATGCTAAAAATGAATTTATATTAAc                                           >  2:1580079/1‑98 (MQ=255)
                                                     ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                        <  1:337982/100‑1 (MQ=255)
                                                     ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTAATAAAAAAAAAAAAAAAAAAAAATCAATTTATATTAAcccc                                        <  2:1186753/100‑1 (MQ=255)
                                                        gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                     <  2:971997/100‑1 (MQ=255)
                                                        gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTACATTAACCCCACt                                     <  1:1277478/100‑1 (MQ=255)
                                                         tATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTa                                    <  1:790475/100‑1 (MQ=255)
                                                          aTTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTaa                                   >  1:529145/1‑100 (MQ=255)
                                                            tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATTTTAACCCCACTa                                    >  2:1342515/1‑97 (MQ=255)
                                                             cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                <  1:890731/100‑1 (MQ=255)
                                                              aTTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgt                               >  1:713628/1‑100 (MQ=255)
                                                               ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                              <  1:49925/100‑1 (MQ=255)
                                                                tAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtgn                             <  2:1203205/100‑2 (MQ=255)
                                                                 aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                            >  1:128940/1‑100 (MQ=255)
                                                                 aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                            >  1:128980/1‑100 (MQ=255)
                                                                  aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                           <  1:657105/100‑1 (MQ=255)
                                                                  aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                           >  1:514111/1‑100 (MQ=255)
                                                                    aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                         <  1:258395/100‑1 (MQ=255)
                                                                     tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                        >  1:316348/1‑100 (MQ=255)
                                                                     tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                        <  1:1148946/100‑1 (MQ=255)
                                                                      gaaaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                       >  1:1065979/3‑100 (MQ=255)
                                                                      gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                       >  1:312014/1‑100 (MQ=255)
                                                                       cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                      >  1:948486/1‑100 (MQ=255)
                                                                       cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                      >  2:877864/1‑100 (MQ=255)
                                                                         aaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatn                    <  2:552405/100‑2 (MQ=255)
                                                                            aCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattata                 <  1:54881/100‑1 (MQ=255)
                                                                            aCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattata                 <  1:54845/100‑1 (MQ=255)
                                                                            aCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattata                 <  1:1355712/100‑1 (MQ=255)
                                                                             cAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                >  1:1096221/1‑100 (MQ=255)
                                                                               aTAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTAATAATATCTACTGCTAAAAATTAATTTATATTTACTTCACTAATAGTGTCTTCATATTATAttt              >  2:1138487/1‑100 (MQ=255)
                                                                               aTAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttt              <  1:609779/100‑1 (MQ=255)
                                                                                  aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt           <  1:57114/100‑1 (MQ=255)
                                                                                   aTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt          <  1:874720/100‑1 (MQ=255)
                                                                                    tCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt         >  2:1311663/1‑99 (MQ=255)
                                                                                      tATGCTTTTGCTATTTTTTTTTGTTTCTAATAATATCTACTTCTAAAAATCAATTTATATTATCCTCACTAATAGTGTTTTCATATTATAttttttttt        >  2:1003716/1‑98 (MQ=255)
                                                                                       aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt      <  1:507345/100‑1 (MQ=255)
                                                                                       aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt      <  1:1519029/100‑1 (MQ=255)
                                                                                          cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg    <  1:942156/99‑1 (MQ=255)
                                                                                          cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa   >  1:224219/1‑100 (MQ=255)
                                                                                           ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac  <  2:161030/100‑1 (MQ=255)
                                                                                           ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac  <  2:73281/100‑1 (MQ=255)
                                                                                                   |                                                                                            
ATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAC  >  NC_000913/2469551‑2469740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: