| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 584,262 | (A)7→8 | coding (582/750 nt) | appY → | global transcriptional activator; DLP12 prophage |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 584,255 | 1 | . | A | 89.8% | 192.3 / 15.5 | 59 | coding (575/750 nt) | appY | global transcriptional activator; DLP12 prophage |
| Reads supporting (aligned to +/- strand): ref base . (1/5); new base A (29/24); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTA > NC_000913/584156‑584347 | cGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGc > 2:1415944/1‑100 (MQ=255)cGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGc > 2:1265278/1‑100 (MQ=255)cGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGc < 1:99518/100‑1 (MQ=255) gaaattaattTAAACAAGTAAAATTTAAAAAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGc < 2:85590/97‑1 (MQ=255) tttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑aaaaaaaac > 1:1471387/1‑98 (MQ=255) ataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGAGACTAGGATGAGGTATGC‑aaaaaaaactca > 2:949281/1‑95 (MQ=255) aCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATa > 2:1532352/1‑100 (MQ=255) aCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATa < 1:1284043/100‑1 (MQ=255) aGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTAAAATATAAAGAGTTAATAGAATGAGGTATGC‑aaaaaaaactca > 2:1425690/1‑90 (MQ=255) agagaaataaAAAAAAGATTAAGGGATGAAGGAACGTCATAGACTGAAATATTGAGAGATACTATGATAAAAAAAAAAAAAAAAA‑‑CTCATAACTTCAAAc < 2:1365342/95‑1 (MQ=255) aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAAc < 2:36778/100‑1 (MQ=255) aataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTa > 1:1178302/1‑100 (MQ=255) aaaaaaaaaaaaTTAACAGATAAACAAACCTCATTTACAAAAATATTCAGATATACCAGGATAAAATAAACAAAAAAAA‑‑CTCATAACTTCAAACTCTTAt < 2:596891/98‑1 (MQ=255) taaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTc < 2:492438/100‑1 (MQ=255) aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTc > 2:197600/1‑100 (MQ=255) aaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAAAAAAAAAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTAt < 2:1289377/100‑1 (MQ=255) aaaaGATTAAGGGATGAAGGAACGTCATTTACGGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATc < 1:377993/100‑1 (MQ=255) aaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCa < 1:988990/100‑1 (MQ=255) aaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAAGAAGAAAAATACAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCa < 1:918943/100‑1 (MQ=255) aaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCaa < 1:235955/100‑1 (MQ=255) aTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGt > 1:125197/1‑100 (MQ=255) ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTc > 2:66644/1‑100 (MQ=255) ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTc > 2:1179929/1‑100 (MQ=255) ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCg < 1:590418/100‑1 (MQ=255) aaGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTa < 1:1082927/100‑1 (MQ=255) gggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAg < 1:555149/100‑1 (MQ=255) ggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGc > 2:110020/1‑100 (MQ=255) ggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGc > 1:713866/1‑100 (MQ=255) ggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGAAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGc < 2:1472811/100‑1 (MQ=255) gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGcc > 2:1352090/1‑100 (MQ=255) tGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCa > 1:546342/1‑100 (MQ=255) gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCTATGTCGTAGCCCAg > 2:1468416/1‑100 (MQ=255) gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAg > 2:339205/1‑100 (MQ=255) aaGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCACACTCTTATTCTATCAATGTCGTAGCCCAGa > 2:369576/1‑100 (MQ=255) aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtg > 1:904777/1‑100 (MQ=255) aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtg > 1:772668/1‑100 (MQ=255) aCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtgt > 1:649785/1‑100 (MQ=255) aCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtgt < 1:470837/100‑1 (MQ=255) gttcttcaCTGAAATATTGAGAGATACTAGAATGTAGTGAGA‑AAAAAAAT‑CTCATAACCTCAAGCTCTTATTCTAACAATGTCGTAGCCCAGAAAtgtg < 2:1416633/92‑1 (MQ=255) gTCATTTACTGAAATATTGAGATATACTAGTATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATTTCGTAGCCCAGAAAtgt < 2:201091/98‑1 (MQ=255) gTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTgg < 1:308110/100‑1 (MQ=255) tCATTTACTGAAATATTGAGATATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc < 1:1378452/100‑1 (MQ=255) tCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc < 1:197600/100‑1 (MQ=255) cATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCt < 2:1089816/99‑1 (MQ=255) aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCta < 1:1523540/100‑1 (MQ=255) tttACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtat > 1:745964/1‑100 (MQ=255) cTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAAc > 1:1223460/1‑99 (MQ=255) tGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAg < 1:1461370/100‑1 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt > 1:863022/1‑100 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAAACCTAAAAACTCCAAACCTTAATTCTAT‑AATTTATTTACCCAGAAAAGTgggatt > 1:478869/1‑90 (MQ=255) aaaTATTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTa < 1:1485958/100‑1 (MQ=255) atatTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACt < 2:1564187/100‑1 (MQ=255) atTGAGAGATACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc > 1:790192/1‑100 (MQ=255) gagagaTACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcata > 1:800557/1‑100 (MQ=255) agaTACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatattt > 1:489252/1‑100 (MQ=255) agaTACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatattt > 1:280997/1‑100 (MQ=255) gaTACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc < 2:1356763/100‑1 (MQ=255) tACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcat > 2:1196258/1‑100 (MQ=255) tACTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttca < 1:1265283/99‑1 (MQ=255) aCTAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata < 1:803157/100‑1 (MQ=255) tAGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtg < 1:1223084/100‑1 (MQ=255) aGGATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgt > 1:18515/1‑100 (MQ=255) gATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGc > 2:570748/1‑100 (MQ=255) gATGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGc > 2:570761/1‑100 (MQ=255) tGAGGTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAt > 2:1134590/1‑100 (MQ=255) ggTATGCAAAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTa > 2:1407170/1‑100 (MQ=255) | CGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAAA‑‑CTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTA > NC_000913/584156‑584347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |