Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,530,076 (A)7→8 coding (155/483 nt) ydcD → putative immunity protein for RhsE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,0691.A95.0% 204.9 / 3.9 60coding (148/483 nt)ydcDputative immunity protein for RhsE
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base A (27/30);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGC  >  NC_000913/1529970‑1530162
                                                                                                     |                                                                                             
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                <  2:195696/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                <  1:895278/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                >  1:1570005/1‑100 (MQ=255)
 gTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGa                                                                                              >  1:109254/1‑100 (MQ=255)
 gTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGa                                                                                              >  2:436167/1‑100 (MQ=255)
      tttGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaa                                                                                         <  1:670324/100‑1 (MQ=255)
        tgttgtTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaa                                                                                       >  2:551630/1‑99 (MQ=255)
         gttgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaat                                                                                      <  2:1214670/100‑3 (MQ=255)
           tgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaatat                                                                                    >  1:642053/1‑96 (MQ=255)
                 ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattta                                                                               >  1:1447697/1‑100 (MQ=255)
                 ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattta                                                                               <  1:599202/100‑1 (MQ=255)
                  cTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattta                                                                               >  2:571954/1‑99 (MQ=255)
                   ttGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTtata                                                                             >  2:1266457/1‑100 (MQ=255)
                       gTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAaat                                                                         >  2:1178070/1‑100 (MQ=255)
                           gATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATAAAAAAAAAAAAAAAAATATTATTTATAAaataat                                                                      <  2:109254/99‑1 (MQ=255)
                           gATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACAAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaatt                                                                     <  2:657005/100‑1 (MQ=255)
                             tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaattaa                                                                   >  2:275575/1‑100 (MQ=255)
                             tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaattaa                                                                   >  1:1393542/1‑100 (MQ=255)
                              aaCAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaattaat                                                                  >  2:1454916/1‑100 (MQ=255)
                               aCAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATg                                                                 >  1:337504/1‑100 (MQ=255)
                                  aTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGAcc                                                              <  1:1286379/100‑1 (MQ=255)
                                   ttCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGAcc                                                              >  2:1176911/1‑99 (MQ=255)
                                      aaaTTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCaaaa                                                          <  1:1170897/100‑1 (MQ=255)
                                        aTTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCaaaaaa                                                        <  1:948447/100‑1 (MQ=255)
                                           cTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATta                                                     >  1:277106/1‑100 (MQ=255)
                                           cTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATta                                                     <  1:1034004/100‑1 (MQ=255)
                                              aCATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATAAAAAAAAAAAAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagta                                                  <  1:649740/100‑1 (MQ=255)
                                              aCAACGTATATTATGGAAATAAAAAACAAAAGAGGGTTGAGATATAAATTTCAGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagt                                                   <  2:448403/99‑1 (MQ=255)
                                                aTCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagtag                                                 <  2:1272649/99‑1 (MQ=255)
                                                aTCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTaga                                                >  1:549300/1‑100 (MQ=255)
                                                 tCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTagag                                               <  2:642051/100‑1 (MQ=255)
                                                  cGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAgg                                              >  1:1316875/1‑100 (MQ=255)
                                                   gTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAggg                                             <  1:976689/100‑1 (MQ=255)
                                                    tATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGa                                            <  1:436168/100‑1 (MQ=255)
                                                     aTTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAAt                                          >  1:1539552/1‑100 (MQ=255)
                                                      ttttATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAAt                                          <  1:381531/100‑1 (MQ=255)
                                                       tttATGGACATAAAACACCAATGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTCTAAAATGATTAATGACCAACAAACTAGTatagtgcat                                          >  2:32462/1‑94 (MQ=255)
                                                         tATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGt                                       <  1:1266462/100‑1 (MQ=255)
                                                         tATGGACATAAAAAACTAAAGAGCGTTGAAATATAACTATCTCAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATgg                                        <  2:210216/99‑1 (MQ=255)
                                                          aaagaaaaaaaaCACCAAAGAGAGTTAAGATAAAACAATCTAAAAAAAAATATTATTTATAAAAAAAAAAAAAAAAAAAAAATTAGTAGAGGGAATGGt                                       <  2:1403027/91‑1 (MQ=255)
                                                             gACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   <  1:1178075/100‑1 (MQ=255)
                                                             gACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   >  2:713586/1‑100 (MQ=255)
                                                             gACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   >  1:1384231/1‑100 (MQ=255)
                                                             gACATAAAACACAAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   <  2:606844/100‑1 (MQ=255)
                                                              aCATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAttt                                  <  1:20668/100‑1 (MQ=255)
                                                               cATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtttt                                 <  1:194820/100‑1 (MQ=255)
                                                               cATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtttt                                 >  1:827712/1‑100 (MQ=255)
                                                                aTAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTta                                >  1:449618/1‑100 (MQ=255)
                                                                  aaaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtata                              <  1:571955/100‑1 (MQ=255)
                                                                   aaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGTGGTAATGGTCATTTTATaa                             >  2:1354480/1‑100 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  1:1345082/100‑1 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  1:699974/100‑1 (MQ=255)
                                                                     acacCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATTACCAAAAAATTAGTTGAGGGAATGGTCTTTTTATAAg                            >  2:1228566/1‑99 (MQ=255)
                                                                     acacCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGt                           >  1:933306/1‑100 (MQ=255)
                                                                      cacCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGta                          >  1:800046/1‑100 (MQ=255)
                                                                        ccAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGtata                        <  1:1186149/100‑1 (MQ=255)
                                                                               gCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTta                 >  1:427192/1‑100 (MQ=255)
                                                                                   tGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaataa             >  2:344450/1‑100 (MQ=255)
                                                                                   tGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaat               <  2:1569995/98‑1 (MQ=255)
                                                                                   tGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTaataat            <  1:518329/100‑1 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          <  2:186189/100‑1 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          >  2:1098650/1‑100 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          >  1:998740/1‑100 (MQ=255)
                                                                                        tatatCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACa        <  2:1467584/100‑1 (MQ=255)
                                                                                        tatatCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACa        >  2:967604/1‑100 (MQ=255)
                                                                                            tCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGAc    <  2:449617/100‑1 (MQ=255)
                                                                                            tCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGAc    <  1:617474/100‑1 (MQ=255)
                                                                                              tttCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGc  <  2:1384223/100‑1 (MQ=255)
                                                                                               ttCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATGAGTAGAGGGAATGGTCATTTTATAAGGATAATGGTTAATAATTACAGGACGc  >  2:1260512/1‑99 (MQ=255)
                                                                                                     |                                                                                             
TGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGC  >  NC_000913/1529970‑1530162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: