Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,564,525 | G→A | M1M (ATG→ATA) † | yffS → | CPZ‑55 prophage; uncharacterized protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,564,525 | 0 | G | A | 97.1% | 232.9 / ‑2.1 | 69 | M1M (ATG→ATA) † | yffS | CPZ‑55 prophage; uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (39/28); total (40/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGC > NC_000913/2564430‑2564620 | gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCATTGGATTCTATGAGATGGGAGATAAAAATCATAAGtt > 2:1543250/1‑100 (MQ=255) gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGtt > 2:917881/1‑100 (MQ=255) gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt > 1:760036/1‑100 (MQ=255) gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt > 1:79104/1‑100 (MQ=255) aTCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTa < 1:242888/100‑1 (MQ=255) tCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTAc < 1:675524/100‑1 (MQ=255) cTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACg > 2:675007/1‑100 (MQ=255) tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGa > 1:190104/1‑100 (MQ=255) tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGa < 1:415607/100‑1 (MQ=255) tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGa < 1:1392320/100‑1 (MQ=255) gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaa < 1:1493198/100‑1 (MQ=255) cAATATTCCCTTCCGTTACGCTTACCCTCCACCCATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCa > 1:512350/1‑100 (MQ=255) cAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCa > 2:448935/1‑100 (MQ=255) atTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAt < 2:1439594/100‑1 (MQ=255) tCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAttt < 2:714927/100‑1 (MQ=255) cccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTg < 2:517927/100‑1 (MQ=255) ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGc > 1:1538435/1‑100 (MQ=255) tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc > 2:554353/1‑100 (MQ=255) ttACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATCAAAATCATAAGTTACGAAATCAAAATTTGCGACATcc < 2:848069/100‑1 (MQ=255) tACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCt < 2:1284297/100‑1 (MQ=255) aCGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTc > 2:1248622/1‑100 (MQ=255) aCGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTc > 2:1547702/1‑100 (MQ=255) cGCTTACCCCCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCa < 2:1207991/100‑1 (MQ=255) gCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa > 1:512755/1‑100 (MQ=255) cTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaaa > 1:730540/1‑100 (MQ=255) cgatcTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAgg < 1:1285729/96‑1 (MQ=255) aCCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc > 2:367738/1‑100 (MQ=255) cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg > 1:905241/1‑100 (MQ=255) cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg > 1:712584/1‑100 (MQ=255) cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg > 1:492898/1‑100 (MQ=255) cTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCt > 2:1147828/1‑100 (MQ=255) ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAggagatcg > 2:1285724/1‑94 (MQ=255) ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTg < 2:1456429/99‑1 (MQ=255) ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc < 2:164470/100‑1 (MQ=255) ccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt > 2:800349/1‑100 (MQ=255) caATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATg < 2:760034/100‑1 (MQ=255) tACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGaa > 2:1303698/1‑100 (MQ=255) cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg < 2:1280818/100‑1 (MQ=255) cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg > 1:1453437/1‑100 (MQ=255) cgTGTTAACCTCCCAATGGGTTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGAc > 1:433028/1‑100 (MQ=255) gtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAat < 1:930/100‑1 (MQ=255) aaCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata > 1:644044/1‑100 (MQ=255) aaCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata < 2:1357566/100‑1 (MQ=255) cTCCCAATGGATTCTATGAGATCGGAGATAAAAATCATAAGTTACGCAATCAAAATTTGCGACATCCTCAAACGCGCTGCGATGGAAGGACATTATAAg > 2:1348213/1‑99 (MQ=255) cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc > 2:56472/1‑100 (MQ=255) cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc > 1:1392160/1‑100 (MQ=255) cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg > 2:367990/1‑99 (MQ=255) cAATGGATTCTATGAGATGGCAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc > 2:965657/1‑100 (MQ=255) aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt > 1:1161535/1‑100 (MQ=255) aTGGATTCTATTAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAGTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTc < 2:997703/100‑1 (MQ=255) ggATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCaa < 2:644042/100‑1 (MQ=255) aTGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGGCAATATAAGGGCGCTCAACGTgggg > 1:1480695/1‑100 (MQ=255) tGAGATGGGAGATAAAAATCATAAGTTACGATATCAAGATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGc < 2:1065696/100‑1 (MQ=255) tGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg > 2:1084223/1‑99 (MQ=255) tGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGc < 2:1501272/100‑1 (MQ=255) gagaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGcc < 2:427432/100‑1 (MQ=255) gagaTGGGAGATAAAAATCATAAGCTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCCGCGATGGAAGGACAATATAAGGGCGCGCAACGTGGGGcc > 2:1473891/1‑100 (MQ=255) agaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCa < 1:1341103/100‑1 (MQ=255) ggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAACTGc > 1:120730/1‑100 (MQ=255) agaTAAAAATCATAAGTTACGAAATCAAAATTTGAGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg > 2:717759/1‑97 (MQ=255) gaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag < 1:1309811/100‑1 (MQ=255) aTAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagg < 1:3704/100‑1 (MQ=255) aTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGTGCTCAACGTGGGGCCAAATGCgagg < 1:186938/100‑1 (MQ=255) aTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCACTCAACGTGGGGCCAAATGCgagg < 2:57406/100‑1 (MQ=255) tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga > 2:873476/1‑100 (MQ=255) aaaaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgaggag > 2:933430/1‑100 (MQ=255) aaaaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgaggag > 2:1155707/1‑100 (MQ=255) aaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGAt > 1:1286073/1‑100 (MQ=255) tCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGc < 1:448936/100‑1 (MQ=255) | GATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGC > NC_000913/2564430‑2564620 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |