Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,037	C→A	A206S (GCG→TCG)	bamB ←	BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,037	0	C	A	100.0%	186.9 / NA	56	A206S (GCG→TCG)	bamB	BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/25);  total (31/25)

AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTC  >  NC_000913/2637941‑2638131                                                                                                 |                                                                                               aaCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAAcc                                                                                             <  2:15760/100‑1 (MQ=255)     ggTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCaaaa                                                                                         <  1:1507693/100‑1 (MQ=255)           cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACGAAAAGCCGt                                                                                    >  1:406072/1‑99 (MQ=255)               gAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCg                                                                               >  2:84471/1‑100 (MQ=255)                 aaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgc                                                                             <  1:178542/100‑1 (MQ=255)                   tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcgc                                                                           <  2:184372/100‑1 (MQ=255)                    aCGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCa                                                                          >  2:292935/1‑100 (MQ=255)                     cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAg                                                                         >  1:1487702/1‑100 (MQ=255)                       ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGAc                                                                       <  2:155074/100‑1 (MQ=255)                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                  <  2:897262/100‑1 (MQ=255)                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                  >  2:253174/1‑100 (MQ=255)                              cAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCAc                                                                >  2:1307899/1‑100 (MQ=255)                               aaaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACTAAAAGCCGTTGTCGGCGCAGACTCGCCACg                                                               <  2:613045/100‑1 (MQ=255)                                aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                              <  1:235959/100‑1 (MQ=255)                                 atcatcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                                             <  2:587080/100‑1 (MQ=255)                                    atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                          <  2:1266042/100‑1 (MQ=255)                                    atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                          >  1:1422565/1‑100 (MQ=255)                                      cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                        <  1:1384406/100‑1 (MQ=255)                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCTCGACAaga                                                         >  2:555509/1‑98 (MQ=255)                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                       >  2:531102/1‑100 (MQ=255)                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTAGGCGCAGACTCGCCACGCaa                                                            >  2:110070/1‑95 (MQ=255)                                        ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCGCGCAAagagag                                                      <  2:714319/100‑1 (MQ=255)                                        ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                      >  2:1047478/1‑100 (MQ=255)                                             tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCACCACGCAAAGAGAGCGAAg                                                 <  1:160151/100‑1 (MQ=255)                                              gTTCCAGCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAgg                                                <  2:820595/100‑1 (MQ=255)                                                tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTATCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                              <  1:1117889/100‑1 (MQ=255)                                                 caaTCAGCACTGCGCTGACGCGACCATTATCGACCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCat                                             >  2:919251/3‑100 (MQ=255)                                                  cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                            >  2:27793/1‑100 (MQ=255)                                                   aTCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCatat                                           >  1:145857/1‑100 (MQ=255)                                                   aTCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCatat                                           >  1:460362/1‑100 (MQ=255)                                                     cAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCg                                         >  2:128645/1‑100 (MQ=255)                                                      aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGa                                        >  1:442959/1‑100 (MQ=255)                                                       gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                       >  1:162262/1‑100 (MQ=255)                                                       gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                       <  1:292935/100‑1 (MQ=255)                                                          cTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                    >  1:1539455/1‑100 (MQ=255)                                                           tGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTa                                   >  1:264149/1‑100 (MQ=255)                                                            gcgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTaa                                  >  2:470323/1‑100 (MQ=255)                                                             cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                 <  1:1238070/100‑1 (MQ=255)                                                             cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                 <  1:1307905/100‑1 (MQ=255)                                                                   cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTcc                            <  2:157329/99‑1 (MQ=255)                                                                   cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                           >  1:996446/1‑100 (MQ=255)                                                                   cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                           >  1:490825/1‑100 (MQ=255)                                                                     cgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCCAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAtt                         >  2:4655/1‑100 (MQ=255)                                                                       aCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                       >  1:271131/1‑100 (MQ=255)                                                                        ccATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGa                      >  1:1383722/1‑100 (MQ=255)                                                                         cATTATCGCCCCCCACGACGGACGAACCAAAAGCCGTTGTCGGCGCCGACTCGCCACGCAAAGAGCGCGAAGGCATATCGAGGTGAACTGTCAAttt                        >  2:1290089/1‑97 (MQ=255)                                                                            tATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgc                  >  2:821645/1‑100 (MQ=255)                                                                                  cccccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTc            <  1:84471/100‑1 (MQ=255)                                                                                   ccccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCa           >  1:308322/1‑100 (MQ=255)                                                                                     ccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  1:519385/100‑1 (MQ=255)                                                                                     ccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  1:315884/100‑1 (MQ=255)                                                                                      cACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc         <  2:1138259/99‑1 (MQ=255)                                                                                      cACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt        >  1:1480901/1‑100 (MQ=255)                                                                                        cgacgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTc      >  1:669729/1‑100 (MQ=255)                                                                                          acgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGt    <  2:490824/100‑1 (MQ=255)                                                                                            ggCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTc  <  2:631173/100‑1 (MQ=255)                                                                                                 |                                                                                               AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTC  >  NC_000913/2637941‑2638131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑