Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,756	G→A	intergenic (+77/‑14)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,756	0	G	A	100.0%	164.0 / NA	49	intergenic (+77/‑14)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/28);  total (21/28)

AGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCC  >  NC_000913/2810661‑2810851                                                                                                |                                                                                                aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                             <  1:1145747/100‑1 (MQ=255) aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                             <  1:635264/100‑1 (MQ=255) aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                             <  1:576182/100‑1 (MQ=255)  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTTTAATTATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                            >  2:210419/1‑100 (MQ=255)  gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                            <  2:1336580/100‑1 (MQ=255)      tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                        >  1:1526267/1‑100 (MQ=255)      tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                        >  1:253214/1‑100 (MQ=255)       ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAcc                                                                                       <  2:1311216/100‑1 (MQ=255)             tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                 <  2:797951/100‑1 (MQ=255)             tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                 <  1:971144/100‑1 (MQ=255)             tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                 <  1:126281/100‑1 (MQ=255)                  aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                            >  1:1420492/1‑100 (MQ=255)                     ccACAAGAATCATTTTTTTTTTTTAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATTGATAGTTc                                                                         >  2:1456845/1‑100 (MQ=255)                      cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTTTATCGTCTGAAATTAATAAGGTCATACCCAAATGTATAGTTc                                                                         >  2:169069/1‑99 (MQ=255)                      cacaAGAATCATTTTTCTAAAACAATACATTTACTTCATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                        <  1:1109556/100‑1 (MQ=255)                       acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTGATACCCAAATGGATAGTTCGt                                                                       >  2:1205617/1‑100 (MQ=255)                          aGAATCATTTTTCTAAAACAATACATTTACTTGATTGGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTa                                                                    <  1:322805/100‑1 (MQ=255)                           gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                   >  1:814722/1‑100 (MQ=255)                           gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                   <  2:654022/100‑1 (MQ=255)                             aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGc                                                                 >  1:1336739/1‑100 (MQ=255)                              tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGcc                                                                <  1:671969/100‑1 (MQ=255)                               cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGccc                                                               <  1:346571/100‑1 (MQ=255)                                aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                              <  2:1475024/100‑1 (MQ=255)                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                        <  1:1580462/100‑1 (MQ=255)                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                        <  2:1320251/100‑1 (MQ=255)                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                        <  2:1454859/100‑1 (MQ=255)                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATACTTCGTTTACACCCATTGaa                                                         >  2:37586/1‑99 (MQ=255)                                        aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                      <  2:1520874/100‑1 (MQ=255)                                                  aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                            >  2:226546/1‑100 (MQ=255)                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                        <  2:311614/100‑1 (MQ=255)                                                        tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                      <  2:349026/100‑1 (MQ=255)                                                        tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                      <  1:575825/100‑1 (MQ=255)                                                        tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                      <  2:451493/100‑1 (MQ=255)                                                              tGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                >  1:673154/1‑100 (MQ=255)                                                                 cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                             <  1:28106/100‑1 (MQ=255)                                                                 cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                             >  1:577217/1‑100 (MQ=255)                                                                           aCTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                   >  2:1005171/1‑100 (MQ=255)                                                                            cTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                  <  2:863222/100‑1 (MQ=255)                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                >  1:1458292/1‑100 (MQ=255)                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                <  2:133273/100‑1 (MQ=255)                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                <  2:23904/100‑1 (MQ=255)                                                                                atCGTCTGAAATTAATAATGTCATACCCAAATTGATAGTTCGTTTACTCCCATTGAACAAATGCTAAAATTTCGCTCCAGCCGCCACGAAGTTTTTc                 <  2:939935/97‑1 (MQ=255)                                                                                   ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc           >  2:145425/1‑100 (MQ=255)                                                                                     cTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg         >  2:1140396/1‑100 (MQ=255)                                                                                     cTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg         >  1:1536785/1‑100 (MQ=255)                                                                                      tcaaaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACCAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg        >  1:282062/3‑100 (MQ=255)                                                                                      tGAAATTAATAAGTTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg        >  2:974615/1‑100 (MQ=255)                                                                                       gAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga       <  2:1526258/100‑1 (MQ=255)                                                                                       gAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga       >  1:869583/1‑100 (MQ=255)                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc  >  1:1378388/1‑100 (MQ=255)                                                                                                |                                                                                                AGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCC  >  NC_000913/2810661‑2810851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑