| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,887,352 | T→A | intergenic (‑133/+226) | ygcB ← / ← cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,887,352 | 0 | T | A | 95.7% | 228.7 / 0.4 | 70 | intergenic (‑133/+226) | ygcB/cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base A (35/32); total (38/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
CAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887258‑2887447 | cAGCGGGCTATTTTTTTTTTTTTTTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 2:1410753/1‑100 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 1:590963/100‑1 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 1:460856/1‑100 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 2:818252/100‑1 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 2:346776/100‑1 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 1:213572/1‑100 (MQ=255)cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 1:1550592/100‑1 (MQ=255) aGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTgg < 1:827155/100‑1 (MQ=255) cGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGt > 1:1035279/1‑100 (MQ=255) gCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCg < 2:594302/100‑1 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACTTTCGAATAGTTTGGGTTCGAACGCTg < 1:688205/100‑1 (MQ=255) ttCGATGCTGCTCAGAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTg < 2:1075763/100‑1 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 1:1487581/1‑100 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 2:244513/1‑100 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 1:912460/1‑100 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc < 1:340481/100‑1 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGc > 1:1276655/1‑99 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAg < 1:1562047/100‑1 (MQ=255) ctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGtt > 2:1115122/1‑100 (MQ=255) tCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTg > 2:1129234/1‑100 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 2:1255128/1‑100 (MQ=255) aTAACTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 2:1145578/1‑99 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:237751/100‑1 (MQ=255) aaaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAtcg > 2:962007/1‑98 (MQ=255) atCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAat > 2:807350/1‑100 (MQ=255) gAATTACAGTGATATACACATGTCTTGCTTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTTGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc > 2:232485/1‑100 (MQ=255) tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 2:870961/1‑100 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTg < 1:1171477/100‑1 (MQ=255) cAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgg < 1:226454/100‑1 (MQ=255) aGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTggg < 2:1487572/100‑1 (MQ=255) aGTGATATAAACATGTCTTGCCTCACTGCAAAGAAGATACTAACATGCGAATAGTATAGGTTCGAGCGCTGGCCTCAGGTTGATAGAAATATCGCCTggg < 2:110638/100‑1 (MQ=255) gTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgggg > 1:1373324/1‑100 (MQ=255) tGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGc < 1:69742/100‑1 (MQ=255) tGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGc > 2:511192/1‑100 (MQ=255) gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 2:1433327/1‑100 (MQ=255) tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt < 1:1427624/100‑1 (MQ=255) tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt < 1:1958/100‑1 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTAGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg > 1:1108846/1‑100 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACACTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg < 2:851903/100‑1 (MQ=255) acacATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGt < 1:34212/100‑1 (MQ=255) acaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:990009/1‑100 (MQ=255) caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa < 1:1452654/100‑1 (MQ=255) cttCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATc < 2:757489/98‑1 (MQ=255) gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 1:694491/100‑1 (MQ=255) gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 2:1373317/100‑1 (MQ=255) tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATcag > 1:757491/1‑98 (MQ=255) tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTg < 1:1551218/100‑1 (MQ=255) cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTgg > 1:753271/1‑100 (MQ=255) ncTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTACGAACGCTGGCCTCAGGTTAATAGCAATATCGACTGGGGCTTTTGTCAATCTGGa > 2:862355/2‑97 (MQ=255) cTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAcc < 2:1043157/99‑1 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 2:164076/1‑100 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 1:766257/1‑100 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 1:630210/1‑100 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgc > 2:800425/1‑99 (MQ=255) cTGCAAAGAAGAGACTAACATACGAATAAGTTGGGTTCGAACGCTAGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcacg > 2:151740/1‑100 (MQ=255) cAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCATCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACATCGCGAAt > 2:639221/1‑100 (MQ=255) aagaagAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGc < 1:557374/100‑1 (MQ=255) agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:1203383/100‑1 (MQ=255) gaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGTAACCTCGCGAATGc > 2:880175/1‑98 (MQ=255) agagACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTaa > 2:273633/1‑100 (MQ=255) gagaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAAc < 1:244513/100‑1 (MQ=255) gaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGc > 1:1279627/1‑100 (MQ=255) gaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGc > 1:440640/1‑100 (MQ=255) aCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 2:1222742/100‑1 (MQ=255) cTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCa > 1:585583/1‑100 (MQ=255) aaCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGa > 2:115976/1‑100 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa < 1:1410761/100‑1 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:985705/1‑100 (MQ=255) aTTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGGATGCTTAACGCCAGAc > 2:1338117/1‑98 (MQ=255) gAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTc < 2:1561405/100‑1 (MQ=255) | CAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887258‑2887447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |