| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,041,028 | G→A | R15C (CGT→TGT) | sdhE ← | flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,028 | 0 | G | A | 95.0% | 187.9 / 0.8 | 60 | R15C (CGT→TGT) | sdhE | flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (25/32); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
CAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGC > NC_000913/3040933‑3041124 | cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg < 2:1576124/100‑1 (MQ=255)cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg < 2:275787/100‑1 (MQ=255)cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg > 1:254911/1‑100 (MQ=255) cGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATg < 2:227581/100‑1 (MQ=255) aaTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGcc > 1:20595/1‑100 (MQ=255) aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc < 2:767954/100‑1 (MQ=255) tAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCa < 2:1497862/100‑1 (MQ=255) tAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCa > 2:773005/1‑100 (MQ=255) tAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCa < 2:231608/100‑1 (MQ=255) aaGATGCGTTTTTTTTTTTTTCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat > 2:1099907/1‑100 (MQ=255) aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaat < 2:189600/100‑1 (MQ=255) aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg < 1:1245590/100‑1 (MQ=255) tGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaat > 1:67938/1‑100 (MQ=255) gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaatg < 1:1438954/100‑1 (MQ=255) tttttCGTCATCGCTTAAGCTGTCGTACAAAAGTACGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCgg < 2:540699/100‑1 (MQ=255) cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCttt > 2:1425454/1‑100 (MQ=255) gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttg < 1:686798/100‑1 (MQ=255) gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttg < 2:470101/100‑1 (MQ=255) cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt > 2:797608/1‑100 (MQ=255) tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTtgttgt < 1:1355643/100‑1 (MQ=255) cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt > 1:335896/1‑100 (MQ=255) cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt < 2:191714/100‑1 (MQ=255) cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt < 1:1099912/100‑1 (MQ=255) cGCTTAAGCTGTCGTACTCATGCTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt > 2:606486/1‑100 (MQ=255) gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa < 2:31808/100‑1 (MQ=255) gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa < 2:185313/100‑1 (MQ=255) gCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTcc < 1:122187/100‑1 (MQ=255) cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa > 1:357479/1‑100 (MQ=255) tGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAt > 2:1293194/1‑100 (MQ=255) cGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct > 1:65930/1‑99 (MQ=255) tCATGTTCGAAAAACGGCATGATTGAAATATCTTGTTCGCTCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc < 2:1160668/100‑1 (MQ=255) tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc < 1:273697/100‑1 (MQ=255) tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc < 2:335896/100‑1 (MQ=255) cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt > 1:761501/1‑100 (MQ=255) aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg > 1:608927/1‑100 (MQ=255) aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg > 1:1255088/1‑100 (MQ=255) gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGGCCATCTTCTTCCTGtc > 1:866441/1‑100 (MQ=255) ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct < 1:912241/100‑1 (MQ=255) cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa > 1:799330/1‑100 (MQ=255) gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc < 1:1127908/100‑1 (MQ=255) gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc < 1:100841/100‑1 (MQ=255) gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc > 1:162056/1‑100 (MQ=255) gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGCCTCAc > 1:329934/1‑100 (MQ=255) aaaaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACg < 2:1358304/100‑1 (MQ=255) cGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAAt > 2:1456964/1‑100 (MQ=255) gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc < 2:612542/100‑1 (MQ=255) aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg < 1:1172208/100‑1 (MQ=255) aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg < 2:167570/100‑1 (MQ=255) aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg > 1:483657/1‑100 (MQ=255) tGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAccc > 2:1393900/1‑100 (MQ=255) gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg > 1:356797/1‑100 (MQ=255) aaTATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc < 2:1097011/100‑1 (MQ=255) gAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTTCCCGGCTATTGt < 1:1036432/100‑1 (MQ=255) aGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa < 1:1514966/100‑1 (MQ=255) gTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTaa > 1:96803/1‑100 (MQ=255) ttCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCTGCTATTGTAAc < 2:548060/100‑1 (MQ=255) ttCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAAc < 2:1560741/100‑1 (MQ=255) tCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg < 1:223648/100‑1 (MQ=255) cgcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtgt < 1:1425462/100‑1 (MQ=255) cATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttt > 2:375472/1‑100 (MQ=255) aCCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTc < 2:1307891/100‑1 (MQ=255) aCCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTc > 1:643242/1‑100 (MQ=255) ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg < 1:1062194/100‑1 (MQ=255) cacaGCGGCATGTCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGTAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGc < 2:371664/100‑1 (MQ=255) cacaGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGc > 1:396091/1‑100 (MQ=255) | CAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGC > NC_000913/3040933‑3041124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |