Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,443,086 T→A N338Y (AAC→TAC)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,0860TA94.1% 221.7 / 2.5 68N338Y (AAC→TAC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (30/34);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT  >  NC_000913/3442990‑3443182
                                                                                                |                                                                                                
ttACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGaa                                                                                               >  1:847126/1‑100 (MQ=255)
       aTCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAAc                                                                                        >  2:1491771/1‑100 (MQ=255)
        tCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACg                                                                                       <  1:422042/100‑1 (MQ=255)
             ataCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtg                                                                                  <  1:733739/100‑1 (MQ=255)
               aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTa                                                                                <  2:1411878/100‑1 (MQ=255)
               aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                                >  1:506433/1‑100 (MQ=255)
               aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                                <  1:324680/100‑1 (MQ=255)
               aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATACACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                                >  2:861573/1‑100 (MQ=255)
                cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag                                                                               >  2:398997/1‑100 (MQ=255)
                cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag                                                                               <  2:610001/100‑1 (MQ=255)
                   cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag                                                                            <  2:1367867/100‑1 (MQ=255)
                   cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                            >  2:1503788/1‑100 (MQ=255)
                   cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                            <  2:981475/100‑1 (MQ=255)
                    gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                           <  2:1552817/100‑1 (MQ=255)
                    gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                           <  1:1322003/100‑1 (MQ=255)
                     ccGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAgaagaa                                                                          <  2:1366936/100‑1 (MQ=255)
                        tttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACa                                                                       <  1:850797/100‑1 (MQ=255)
                         ttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACag                                                                      <  2:161450/100‑1 (MQ=255)
                           gCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaa                                                                    >  2:416624/1‑100 (MQ=255)
                            ctctCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaag                                                                   <  2:625807/100‑1 (MQ=255)
                               tcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaag                                                                >  1:160951/1‑100 (MQ=255)
                               tcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaag                                                                >  1:205333/1‑100 (MQ=255)
                                cccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaaga                                                               >  1:777208/1‑100 (MQ=255)
                                    gACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat                                                           <  1:901013/100‑1 (MQ=255)
                                      cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg                                                         >  1:1251082/1‑100 (MQ=255)
                                       gAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGc                                                        <  1:1270240/100‑1 (MQ=255)
                                        aaTTCCTGGTACAAATGCACCGGATTTCTTCAGGTTATCTGCTTTGTCACGCGGATAGAAAACCAACTCCTTGTAGAAGAAACAGAAGAAGATGATTGc                                                        <  2:318823/99‑1 (MQ=255)
                                        aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCa                                                       <  1:878073/100‑1 (MQ=255)
                                        aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCa                                                       <  1:877969/100‑1 (MQ=255)
                                         aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg                                                      <  2:930718/100‑1 (MQ=255)
                                         aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg                                                      <  1:192768/100‑1 (MQ=255)
                                             cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                  <  1:1413658/100‑1 (MQ=255)
                                             cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                  >  2:1453511/1‑100 (MQ=255)
                                              tGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCAGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCa                                                 <  1:1503797/100‑1 (MQ=255)
                                                     aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                          >  2:712456/1‑100 (MQ=255)
                                                       tGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAac                                        <  2:526249/100‑1 (MQ=255)
                                                       tGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAac                                        <  1:1362837/100‑1 (MQ=255)
                                                         cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                      <  1:1491780/100‑1 (MQ=255)
                                                         cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                      <  1:1080233/100‑1 (MQ=255)
                                                         cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                      <  1:218179/100‑1 (MQ=255)
                                                          aCCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca                                     <  2:160951/100‑1 (MQ=255)
                                                           ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca                                     >  2:1110033/1‑99 (MQ=255)
                                                           ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAAcaca                                     >  2:1494461/1‑99 (MQ=255)
                                                           ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                    >  1:458188/1‑100 (MQ=255)
                                                           ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                    >  1:380832/1‑100 (MQ=255)
                                                               aCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAg                                >  1:141581/1‑100 (MQ=255)
                                                                cttcttCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGc                               >  1:435588/1‑100 (MQ=255)
                                                                  tcttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAATACATAAAGCgg                             <  2:1226569/100‑1 (MQ=255)
                                                                     tcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGGAGAAGAATCAGGAGAAGGTGATTACAGACGGAAAGAGTAACACATAAAACGGtt                           >  2:1065984/1‑99 (MQ=255)
                                                                      cAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc                         >  2:911375/1‑100 (MQ=255)
                                                                      cAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATAATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc                         >  1:1108627/1‑100 (MQ=255)
                                                                       aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        <  1:1347816/100‑1 (MQ=255)
                                                                       aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        >  1:753543/1‑100 (MQ=255)
                                                                          ttATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                     <  2:806523/100‑1 (MQ=255)
                                                                           tATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAgg                    >  1:1506028/1‑100 (MQ=255)
                                                                              ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg                 <  2:1157411/100‑1 (MQ=255)
                                                                              ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATAGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg                 >  2:1194320/1‑100 (MQ=255)
                                                                               tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc                >  2:1474426/1‑100 (MQ=255)
                                                                               tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc                >  1:906961/1‑100 (MQ=255)
                                                                               tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc                >  1:152312/1‑100 (MQ=255)
                                                                                gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa               >  2:1533480/1‑100 (MQ=255)
                                                                                 ctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaa              >  2:796163/1‑100 (MQ=255)
                                                                                  tttttCAAGCGGGTAAAAAAACAACAGCGTGTAAAAAAAACAGAAAAAGATGATTTCAGACGCATAGATTAACACATAAAGCGGTTTCCCAGGCTTCaaa             <  2:998445/98‑1 (MQ=255)
                                                                                  tgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaaa             <  2:1479751/100‑1 (MQ=255)
                                                                                    tttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAAt            <  2:506432/99‑1 (MQ=255)
                                                                                       cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCGTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAg        <  1:727497/100‑1 (MQ=255)
                                                                                         cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg      >  1:140049/1‑100 (MQ=255)
                                                                                             ggTTTAAAACCAACGCCGTGTAGAATAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAt  <  2:1473008/100‑1 (MQ=255)
                                                                                                |                                                                                                
TTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT  >  NC_000913/3442990‑3443182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑