Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,648,042	T→C	intergenic (‑254/‑486)	dinQ ← / → arsR	UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,042	0	T	C	100.0%	229.8 / NA	64	intergenic (‑254/‑486)	dinQ/arsR	UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/24);  total (40/24)

TATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGT  >  NC_000913/3647945‑3648138                                                                                                  |                                                                                                 tATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCAt                                                                                                >  2:1019116/1‑100 (MQ=255)  aTGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATg                                                                                               >  1:152197/1‑100 (MQ=255)   tGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATgg                                                                                              >  2:678248/1‑100 (MQ=255)   tGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATgg                                                                                              <  1:747939/100‑1 (MQ=255)    gcgcATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTTTAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGt                                                                                             <  2:848144/100‑1 (MQ=255)     cgcATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTa                                                                                            <  1:278867/100‑1 (MQ=255)       cATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTAtt                                                                                          >  2:104273/1‑100 (MQ=255)       cATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTAtt                                                                                          <  1:1463861/100‑1 (MQ=255)        aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                         <  1:126235/100‑1 (MQ=255)              agagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTg                                                                                   <  1:1162706/100‑1 (MQ=255)              agagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTg                                                                                   <  1:331339/100‑1 (MQ=255)                 gTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATg                                                                                >  2:1101600/1‑100 (MQ=255)                      aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGttn                                                                           <  1:552388/100‑2 (MQ=255)                       aTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAg                                                                          <  2:310086/100‑1 (MQ=255)                          ccTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatg                                                                       <  2:714721/100‑1 (MQ=255)                          ccTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatg                                                                       >  1:1521101/1‑100 (MQ=255)                           cTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatga                                                                      >  1:705675/1‑100 (MQ=255)                           cTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatga                                                                      >  2:808569/1‑100 (MQ=255)                            tCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgatgat                                                                     >  1:1400896/1‑100 (MQ=255)                                agCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCt                                                                 <  2:458862/100‑1 (MQ=255)                                  cgaaCTGGATAATAATACAGTACATGCAGGTAATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAt                                                               >  1:1063931/3‑100 (MQ=255)                                     aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtctc                                                            <  1:1316710/100‑1 (MQ=255)                                      cTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCa                                                           >  2:39468/1‑100 (MQ=255)                                       tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCAc                                                          >  2:1507372/1‑100 (MQ=255)                                        ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCGATAGTTTCAGCATCGTATTAGCATTCATCCGTTAGATGATGGCTATCGCACt                                                         <  2:1083245/100‑1 (MQ=255)                                        ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACt                                                         >  2:602396/1‑100 (MQ=255)                                        ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACt                                                         >  1:1448512/1‑100 (MQ=255)                                           tAATCGTACAGTCGATGCAGGTTATAAAAGCAGCAAGTGCTTGCATTAGCTGCGTCGTTGTATTAGCATGGATGCGGTATAGGATGACTATCTCAcgc                                                        >  2:684080/1‑96 (MQ=255)                                           tAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCa                                                      >  1:1164603/1‑100 (MQ=255)                                           tAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCa                                                      >  2:323557/1‑100 (MQ=255)                                           tAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCa                                                      >  2:199221/1‑100 (MQ=255)                                             aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                    >  1:1292892/1‑100 (MQ=255)                                             aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                    >  2:258719/1‑100 (MQ=255)                                              tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTc                                                   >  1:619110/1‑100 (MQ=255)                                               cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCa                                                  >  1:1514088/1‑100 (MQ=255)                                                aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                 >  1:771816/1‑100 (MQ=255)                                                aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                 <  1:602398/100‑1 (MQ=255)                                                  aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCagag                                               <  1:996399/100‑1 (MQ=255)                                                  aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCagag                                               >  2:1245517/1‑100 (MQ=255)                                                      tACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccac                                           <  1:522450/100‑1 (MQ=255)                                                        cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacca                                         >  2:3964/1‑100 (MQ=255)                                                        cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacca                                         >  2:1195803/1‑100 (MQ=255)                                                           gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACt                                      >  1:474307/1‑100 (MQ=255)                                                           gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACt                                      >  1:1322038/1‑100 (MQ=255)                                                            cAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGTGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTc                                     >  2:1562900/1‑100 (MQ=255)                                                             aGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCa                                    >  1:257634/1‑100 (MQ=255)                                                              ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAg                                   <  2:152197/100‑1 (MQ=255)                                                              ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAg                                   >  2:76351/1‑100 (MQ=255)                                                              ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAg                                   >  1:1437512/1‑100 (MQ=255)                                                              ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAg                                   <  1:644614/100‑1 (MQ=255)                                                                ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAggg                                 >  1:438084/1‑100 (MQ=255)                                                                    aaaaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCt                               >  2:768121/1‑98 (MQ=255)                                                                     aaaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGa                            >  2:692122/1‑100 (MQ=255)                                                                      aaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGaa                           <  2:1292886/100‑1 (MQ=255)                                                                      aaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGaa                           <  2:1521092/100‑1 (MQ=255)                                                                           gCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGt                        >  2:1024313/1‑98 (MQ=255)                                                                            cACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTaaa                     <  1:273622/100‑1 (MQ=255)                                                                             aCGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTaaaa                    <  1:689445/100‑1 (MQ=255)                                                                              cGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTGGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTaaaaa                   <  1:1080819/100‑1 (MQ=255)                                                                                     gCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACg            <  2:257634/100‑1 (MQ=255)                                                                                       aaTAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCa          >  2:1250069/1‑100 (MQ=255)                                                                                       aaTAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCa          >  1:836400/1‑100 (MQ=255)                                                                                           gTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGt      >  1:288416/1‑100 (MQ=255)                                                                                               cAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt  >  1:1283520/1‑100 (MQ=255)                                                                                                  |                                                                                                 TATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGT  >  NC_000913/3647945‑3648138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑