| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,840,547 | T→C | intergenic (+39/+2) | yicS → / ← nepI | putative periplasmic protein/putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,840,547 | 0 | T | C | 98.3% | 195.8 / ‑4.2 | 60 | intergenic (+39/+2) | yicS/nepI | putative periplasmic protein/putative transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (27/32); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
TAATAATCAGAGCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGATATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCAGACC > NC_000913/3840451‑3840638 | taataaTCAGAGCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATc > 1:477693/1‑100 (MQ=255) aataatCAGAGCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCa < 1:263317/100‑1 (MQ=255) taatCAGAGCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGATATCAgg > 1:837008/1‑100 (MQ=255) aaacAGAGCAATTATGGGGAACCGTTGGGTAAGGTAGAATGCCCGGATAGGTAAGGACAGAAAAAGGTAATGACCAACGCTTCTTTAAATGACATccgca > 2:1417412/4‑96 (MQ=255) agagCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATttc < 1:221493/100‑1 (MQ=255) gagCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATttct > 1:54397/1‑100 (MQ=255) gCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATttcttc < 1:921875/100‑1 (MQ=255) cAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCa > 1:761444/1‑100 (MQ=255) aaTTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCAt > 1:29253/1‑100 (MQ=255) attattGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCAtt < 1:1461939/100‑1 (MQ=255) tattGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGCACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCAtttt < 2:1009081/100‑1 (MQ=255) ttGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCGCCGCTTCTTTACCTGACATCAGGATTTCTTCAttttn < 2:644729/99‑2 (MQ=255) ttGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCa < 1:1184480/100‑1 (MQ=255) ttGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCa < 1:1493528/100‑1 (MQ=255) tGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCAc < 1:814572/100‑1 (MQ=255) gggAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTGCTTCATTTTCAcc < 2:977216/100‑1 (MQ=255) gCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGc < 1:1487201/100‑1 (MQ=255) tGGGTAAGGTAGAATGCCCGGATATCTCAGAACAGAAACAACTCCTCACCCCCGCTTCTTTACCTGACATCACGATTTCCTCATTTTCACCTTTGCAGTa < 2:130308/100‑1 (MQ=255) ggTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAAc > 1:558174/1‑100 (MQ=255) ggTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAAc < 1:209190/100‑1 (MQ=255) gTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAAcc < 1:515836/100‑1 (MQ=255) tAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACca > 2:351248/1‑100 (MQ=255) ggTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACcaaca > 2:952342/1‑100 (MQ=255) ggTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACcaaca > 2:871745/1‑100 (MQ=255) ggTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACcaaca > 1:191637/1‑100 (MQ=255) ggTAGAAGGCCCGGATATGTAAGAGCAGCCACAACTACTGACCAGCGAGTCTTTACCTGACATCAGGATTTCTTCAGTGTCAGCTTTGCAGTAACcaac > 2:704848/1‑99 (MQ=255) gTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACcaac < 1:1014321/98‑1 (MQ=255) aGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATg > 2:233696/1‑100 (MQ=255) aTGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAg > 1:1132884/1‑100 (MQ=255) aTGCCCGGAGATGTAAGAACAGCAACAACTACTGACCAACGCGTCTTTACCTGACATTAGGATTTCTTCATTGTCACCTTTGCAGTAACCAACAATGCAg > 2:1108069/1‑100 (MQ=255) gCCCGGCTATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCCCCTTTGCAGTAACCAACAATGCAGTc > 2:1099514/1‑100 (MQ=255) gccGGATATGTAAGAACAGCGACCACTACTGACCAACGCTTCTTTACCTGACATCAGGGTTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCa > 2:174171/2‑100 (MQ=255) cGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGc < 2:724752/100‑1 (MQ=255) atGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACAt > 2:752398/1‑100 (MQ=255) tGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATc > 1:522598/1‑100 (MQ=255) tGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATc > 2:555049/1‑100 (MQ=255) gTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCa > 2:663425/1‑100 (MQ=255) aaGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAAt < 2:1509940/100‑1 (MQ=255) gAACAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAAtgt > 1:943427/1‑100 (MQ=255) aaCAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAAtgtg < 2:891410/100‑1 (MQ=255) aaCAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAAtgtg < 1:952347/100‑1 (MQ=255) aaCAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAAtgtg > 2:758219/1‑100 (MQ=255) aCAGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGc > 1:502217/1‑100 (MQ=255) aGCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCg < 1:662677/100‑1 (MQ=255) gCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCgg > 2:499366/1‑100 (MQ=255) gCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCgg < 1:1575335/100‑1 (MQ=255) gCAACAACTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCgg > 1:48614/1‑100 (MQ=255) caacaaCTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGa < 2:550424/100‑1 (MQ=255) aacTACTGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGacaac < 2:54397/100‑1 (MQ=255) actactGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGacaaca < 2:869551/100‑1 (MQ=255) ctGACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCaa > 1:463/1‑100 (MQ=255) gACCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACg > 1:342487/1‑100 (MQ=255) aCCAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACgg < 2:558173/100‑1 (MQ=255) cAACGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTACCCAACACTGCAGTCAGCAACATCACTGTGCCGGACAACATCAACGGCg < 2:860920/100‑1 (MQ=255) cGCTTCTTTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAg < 2:343519/100‑1 (MQ=255) ttgttttCCTGTCATCATTATTTCTTCATTTTCTCCTTCGCAGTAACCAACTAGGCAGTCAGCAACATCAATGTGCTGGACAACATCAACGGCGAAg < 2:210927/94‑1 (MQ=255) ttcttTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCa < 2:1140944/100‑1 (MQ=255) tcttTACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCAg < 1:196665/100‑1 (MQ=255) tttACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCAGAc < 2:1219148/100‑1 (MQ=255) ttACCTGACATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCAGAcc < 1:907614/100‑1 (MQ=255) | TAATAATCAGAGCAATTATTGGGAAGCGTTGGGTAAGGTAGAATGCCCGGATATGTAAGAACAGCAACAACTACTGACCAACGCTTCTTTACCTGATATCAGGATTTCTTCATTTTCACCTTTGCAGTAACCAACAATGCAGTCAGCAACATCAATGTGCCGGACAACATCAACGGCGAAGTCAGACC > NC_000913/3840451‑3840638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |