| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 265,418 | A→G | R42R (CGT→CGC) | ykfF ← | CP4‑6 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 265,418 | 0 | A | G | 98.1% | 178.4 / ‑4.2 | 52 | R42R (CGT→CGC) | ykfF | CP4‑6 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (21/30); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGC > NC_000913/265328‑265514 | gATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAaccacc < 1:740631/100‑1 (MQ=255) tGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAg < 2:206782/100‑1 (MQ=255) gCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGa < 1:533752/100‑1 (MQ=255) ccTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGAc < 1:1031208/100‑1 (MQ=255) ccTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGAc < 1:214062/100‑1 (MQ=255) ccTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGAc > 2:1280481/1‑100 (MQ=255) gTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGt < 1:573204/100‑1 (MQ=255) gCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGa > 1:903988/1‑100 (MQ=255) cGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGAc < 2:1127946/100‑1 (MQ=255) cGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGgt > 1:1414526/1‑100 (MQ=255) aggccTTCACCGGCATCCGGCTCAGAATTCCATGCTCGCCAGATCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCtt < 2:207734/97‑1 (MQ=21) gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGCCGGAAGTGACTGCCCTGGTCGACTTc > 1:25611/1‑100 (MQ=255) gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTc > 1:1060435/1‑100 (MQ=255) gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTCCCCTGGTCGTCTTc > 2:855042/1‑100 (MQ=255) ccTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCg > 2:1305397/1‑99 (MQ=255) tCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtg < 1:4293/100‑1 (MQ=255) tCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtg > 1:701044/1‑100 (MQ=255) cACCGGCATCCGGCTCAAAGTTCCAGGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtgt < 2:1420907/100‑1 (MQ=255) ccGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGTCCTGGTCGTCTTCGAGTGTGa < 1:1111892/100‑1 (MQ=255) cGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGtgtg < 2:1231309/98‑1 (MQ=255) ggCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGata > 2:100308/1‑100 (MQ=255) gCATCCGGCTCAAAGTTCCATGCCCGCCAGACCCTCCGGCCTTCAGTATCGCGAACCACCCGACGGGAGTGACTGCCCTGGTCGTCTTCGAGAGTGat > 2:46039/1‑98 (MQ=37) tCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc < 2:623874/100‑1 (MQ=255) tCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc < 1:100308/100‑1 (MQ=255) tCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc > 2:803756/1‑100 (MQ=255) ccGGCTCGAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCt > 1:1405569/1‑100 (MQ=255) cGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTg < 1:698508/100‑1 (MQ=255) cGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTg < 1:1244772/100‑1 (MQ=255) cGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTg < 1:218109/100‑1 (MQ=255) cgaaGTTCCATGCCCGCCAGACCATCGTGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACg < 2:1132579/97‑1 (MQ=37) cAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGt > 1:243618/1‑100 (MQ=255) aaaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTg < 1:717946/100‑1 (MQ=255) aaaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTg < 1:1281201/100‑1 (MQ=255) aaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTgg < 2:1284925/100‑1 (MQ=255) ttCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAg > 2:1177915/1‑100 (MQ=255) ccATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATGTTGCTGTACGTGGTAGTg > 2:644096/1‑100 (MQ=255) ccATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACgtggtggtg > 2:1433957/1‑100 (MQ=255) tGCCCGCCAGCCCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCGTCCAGTGTGATATTGCTGTACGTGGTAGTGAcc < 2:1040002/100‑1 (MQ=255) tGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGAcc < 1:256442/100‑1 (MQ=255) tGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGAcc < 1:631656/100‑1 (MQ=255) tGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGAcc < 2:484897/100‑1 (MQ=255) gCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcg > 2:740967/1‑100 (MQ=255) ccGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTTCGTGGTAGTGACcgct > 2:926051/1‑100 (MQ=255) gACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgct < 1:1503840/100‑1 (MQ=255) gCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCgg < 1:904099/100‑1 (MQ=255) ccTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCggg < 2:1414518/100‑1 (MQ=255) tCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGa > 1:558120/1‑100 (MQ=255) cAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGaa > 2:538067/1‑100 (MQ=255) gTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAg < 2:20714/100‑1 (MQ=255) gTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAg < 2:747254/100‑1 (MQ=255) gTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAg > 2:886793/1‑100 (MQ=255) aTCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGc > 1:997459/1‑100 (MQ=255) | GATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGC > NC_000913/265328‑265514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |