Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,360,814 T→G Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,360,8140TG95.7% 297.0 / 3.2 93Y374S (TAC→TCC) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (51/37);  total (54/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTG  >  NC_000913/4360717‑4360907
                                                                                                 |                                                                                             
gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                             <  1:1510821/100‑1 (MQ=255)
 ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAgg                                                                                            <  2:324080/100‑1 (MQ=255)
  tATCTATTTCTGTGTTCAGTACTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGt                                                                                           >  2:968659/1‑100 (MQ=255)
   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                          >  1:783204/1‑100 (MQ=255)
   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTCTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAGCTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                          >  2:947470/1‑100 (MQ=255)
    tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaa                                                                                         >  1:366192/1‑100 (MQ=255)
    tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaa                                                                                         >  2:94662/1‑100 (MQ=255)
    tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTATCGAGGAGGTaa                                                                                         >  1:1254208/1‑100 (MQ=255)
      tATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAt                                                                                       >  1:607423/1‑100 (MQ=255)
      tATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAt                                                                                       >  2:523175/1‑100 (MQ=255)
          tCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                   <  1:801604/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                 <  1:1298734/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                 >  2:396678/1‑100 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                 <  1:609715/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                 <  1:1193598/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                  >  1:889916/1‑99 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                <  2:690566/100‑1 (MQ=255)
               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                              >  2:469416/1‑100 (MQ=255)
                  cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatgat                                                                           >  1:727351/1‑100 (MQ=255)
                   aGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGAtt                                                                          >  2:1322856/1‑100 (MQ=255)
                      gctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGaa                                                                       >  1:1498533/1‑100 (MQ=255)
                      gctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCGCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                         <  2:1491955/98‑1 (MQ=255)
                       ctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAAc                                                                      <  1:229431/100‑1 (MQ=255)
                       ctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAAc                                                                      >  1:939199/1‑100 (MQ=255)
                         gctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACaa                                                                    <  2:741669/100‑1 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGAGAAGGTAAATTCTGGGGATGATTGAACAAtc                                                                  >  1:538237/1‑100 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                 >  1:1088191/1‑100 (MQ=255)
                             aTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtctc                                                                >  1:239539/1‑100 (MQ=255)
                              ttGTTTTTCATCAAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCa                                                               <  2:1230071/100‑1 (MQ=255)
                               tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCa                                                               <  2:458864/99‑1 (MQ=255)
                                  ttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCta                                                           >  2:1524705/1‑100 (MQ=255)
                                  ttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCAGAGGTAAATTCTGGGGATGATTGAACAATCTCACCta                                                           >  1:916219/1‑100 (MQ=255)
                                      cATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCAGAGGTAAATTCTAGGGATGATTGAACAATCTCACCtaataa                                                       >  2:141176/1‑100 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                    <  2:1120365/100‑1 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                    <  1:968664/100‑1 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                    <  1:1009868/100‑1 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTTGGGATGATTGAACAATCTCACCTAATAATTc                                                    >  1:995153/1‑99 (MQ=255)
                                          tAAAGGATGGTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                    <  2:236645/99‑1 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGTTTGAACAATCTCACCTAATAATTCAc                                                  >  2:727757/1‑100 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                  <  1:1181154/100‑1 (MQ=255)
                                            aaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACt                                                 >  2:1049478/1‑100 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  1:1578975/100‑1 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  1:653788/100‑1 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  2:1084771/100‑1 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  1:396679/100‑1 (MQ=255)
                                               gATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGc                                              <  1:220764/100‑1 (MQ=255)
                                                  gTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACg                                           >  1:1568343/1‑100 (MQ=255)
                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                          >  2:397064/1‑100 (MQ=255)
                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                          >  1:1275153/1‑100 (MQ=255)
                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTATCGCGGCGGAAAATTCTGGGGATTATTGACTCATCTAACCTAATAATTCACTGTCAAgg                                          >  2:1002078/1‑100 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                         >  2:336018/1‑100 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                       <  1:469417/100‑1 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                       <  2:732398/100‑1 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTCTTTCTGCTCTCGCGGAGGTAAACTCTGGGGATGATTAAACAATCTCACATAATAATTCACTggacag                                           >  2:1279081/1‑92 (MQ=255)
                                                      agaATGGACCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAATCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                       <  1:1206297/100‑1 (MQ=255)
                                                       gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCa                                      >  1:1446715/1‑100 (MQ=255)
                                                        aaTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAGCTCACCTAATAATTCACTGGCACGGTTCaa                                     <  2:373223/100‑1 (MQ=255)
                                                         aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                    <  2:241864/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                    <  2:1226825/99‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                   <  1:94662/100‑1 (MQ=255)
                                                          tGGCGAACGATATCAACTAATGCTTTTTCTGCTCTCGGGGAGGTAAAGTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                   <  2:1064618/100‑1 (MQ=255)
                                                           ggCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAtn                                   <  2:381484/99‑2 (MQ=255)
                                                            gcgcCCGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCTCGGt                                         >  2:327163/1‑92 (MQ=255)
                                                            gcgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAt                                 <  2:727349/100‑1 (MQ=255)
                                                            gcgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAt                                 >  1:396348/1‑100 (MQ=255)
                                                            gcgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTAACTGAt                                 >  2:699308/1‑100 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                              >  1:123962/1‑100 (MQ=255)
                                                                 cGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatc                            >  1:764936/1‑100 (MQ=255)
                                                                   atatCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcat                          <  2:396348/100‑1 (MQ=255)
                                                                    tatCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcatc                         >  2:1146042/1‑100 (MQ=255)
                                                                    tatCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcatc                         >  2:1145998/1‑100 (MQ=255)
                                                                     atCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTCATCATCg                        <  1:833482/100‑1 (MQ=255)
                                                                        aaCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                      >  2:847337/1‑99 (MQ=255)
                                                                        aaCTAATGCTTTTTATGCTCTCGCGGAGGTAAATTCTGAGGATGATTAAACAATCTCAAATAATAATTCAATGACACGATTCAATAATTTatcataacca                     >  2:15370/1‑95 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                  <  2:995147/100‑1 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                  >  2:133846/1‑99 (MQ=255)
                                                                              tGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGt               >  2:883823/1‑100 (MQ=255)
                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa              >  2:405205/1‑100 (MQ=255)
                                                                                 tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa            <  1:887202/100‑1 (MQ=255)
                                                                                 tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa            >  2:338356/1‑100 (MQ=255)
                                                                                  ttttCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaat           <  2:235120/100‑1 (MQ=255)
                                                                                  ttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaat           >  1:333988/1‑100 (MQ=255)
                                                                                    ttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCGCTGTCACGGTTCAATGATTTCTTATCGCCATGCCGTAaatga         >  2:1107056/1‑98 (MQ=255)
                                                                                     tCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataat        <  1:62611/100‑1 (MQ=255)
                                                                                      ntGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc       >  1:1257322/2‑100 (MQ=255)
                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCGCACCTAATAATTCACTGGCGCGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc       >  2:862196/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAACTAATCAt     >  1:1362227/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt     >  1:1378437/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt     >  2:1322552/1‑100 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg    >  1:1308592/1‑100 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTATGGGGATGATTGAACAATCTCACCTAATAATTCACTGGTACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa      <  2:658198/98‑1 (MQ=255)
                                                                                          tctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAcgt   >  2:1443679/1‑100 (MQ=255)
                                                                                           ctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgtg  >  1:655139/1‑100 (MQ=255)
                                                                                                 |                                                                                             
GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTG  >  NC_000913/4360717‑4360907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: