Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,214	T→C	D237G (GAC→GGC)	ddlA ←	D‑alanine‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,214	0	T	C	100.0%	230.6 / NA	64	D237G (GAC→GGC)	ddlA	D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/31);  total (33/31)

TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAT  >  NC_000913/400117‑400308                                                                                                  |                                                                                               tACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCg                                                                                              <  1:1513220/100‑1 (MQ=255)  aCCACTTTCGCGCGATCTTCGTCAATGTACTGGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                             >  1:1064226/1‑100 (MQ=255)       tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                        >  1:389078/1‑100 (MQ=255)       tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                        >  1:1387987/1‑100 (MQ=255)        ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCa                                                                                       <  1:220134/100‑1 (MQ=255)          cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGa                                                                                     >  1:376852/1‑100 (MQ=255)          cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGa                                                                                     <  1:276803/100‑1 (MQ=255)           gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                    >  2:1012898/1‑100 (MQ=255)           gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                    >  1:745638/1‑100 (MQ=255)           gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                    >  2:668690/1‑100 (MQ=255)            cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                   <  1:1177584/100‑1 (MQ=255)              ccATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTg                                                                                 >  2:1158859/1‑100 (MQ=255)                aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                               <  2:397815/100‑1 (MQ=255)                aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                               <  1:1453203/100‑1 (MQ=255)                  cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                             >  1:1319729/1‑100 (MQ=255)                   ttCGTCAATGTACTTGGAGTCGTAGTCATAGAAATCGCTGGTGAGTACGCTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAGTGCGCa                                                                             >  2:871489/1‑99 (MQ=255)                    tCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCAtt                                                                           >  2:1068772/1‑100 (MQ=255)                     cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                          >  2:1394147/1‑100 (MQ=255)                      gTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCg                                                                         >  2:1121783/1‑100 (MQ=255)                         aaTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtc                                                                      <  1:1139895/100‑1 (MQ=255)                         aaTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtc                                                                      <  1:1097808/100‑1 (MQ=255)                           tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGCTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                    <  2:880010/100‑1 (MQ=255)                             tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                  >  1:1100461/1‑100 (MQ=255)                              aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                                 >  2:808717/1‑100 (MQ=255)                                ttGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAc                                                               >  1:783/1‑100 (MQ=255)                                      tCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGa                                                         <  1:1230592/100‑1 (MQ=255)                                       cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCAGAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                        >  1:1313609/1‑100 (MQ=255)                                       cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                        >  1:956628/1‑100 (MQ=255)                                       cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                        >  1:416180/1‑100 (MQ=255)                                        gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                       >  2:31705/1‑100 (MQ=255)                                        gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                       <  1:1103142/100‑1 (MQ=255)                                        gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                       <  1:345265/100‑1 (MQ=255)                                          tctcATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATccc                                                     <  1:1262523/97‑1 (MQ=255)                                           ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                    >  2:658553/1‑100 (MQ=255)                                             cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATcccaga                                                  >  2:1262518/1‑97 (MQ=255)                                             cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                  >  1:539300/1‑100 (MQ=255)                                             cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                  >  1:1258368/1‑100 (MQ=255)                                              aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                 <  1:609939/100‑1 (MQ=255)                                               tAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCt                                                >  2:303606/1‑100 (MQ=255)                                                aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               >  2:335684/1‑100 (MQ=255)                                                aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               >  2:17297/1‑100 (MQ=255)                                                aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               <  2:1101843/100‑1 (MQ=255)                                                   aaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                            >  1:379605/1‑100 (MQ=255)                                                    aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACg                                           <  1:1158864/100‑1 (MQ=255)                                                       gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                        <  1:43196/100‑1 (MQ=255)                                                               gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                <  1:1068778/100‑1 (MQ=255)                                                                 aCGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                              >  1:642994/1‑100 (MQ=255)                                                                  cGATCTCCCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                             >  1:520468/1‑100 (MQ=255)                                                                   gATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                            <  1:736763/100‑1 (MQ=255)                                                                    aTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                           <  1:485881/100‑1 (MQ=255)                                                                       tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                         <  2:124895/99‑1 (MQ=255)                                                                         tcCACAGGTGCTGGCTTGCGGATTCCCGTTGCCCAGAACTGCGCATTCCATCTCACTACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                      <  2:99909/100‑1 (MQ=255)                                                                          ccACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                     <  1:503374/100‑1 (MQ=255)                                                                           cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                    <  2:783/100‑1 (MQ=255)                                                                            acaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaac                   >  2:675600/1‑100 (MQ=255)                                                                               ggTGCTGGCTTGCGGATTGCCGCTGCCCAGACCTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc                <  2:1064220/100‑1 (MQ=255)                                                                                   cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt            <  1:253526/100‑1 (MQ=255)                                                                                   cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt            <  2:1387979/100‑1 (MQ=255)                                                                                    tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc                <  2:1497410/95‑1 (MQ=255)                                                                                    tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc           >  2:556221/1‑100 (MQ=255)                                                                                        ttGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg       <  1:1258428/100‑1 (MQ=255)                                                                                          gCGGATTGCCGTTGCCCAGAACTGCGCATTCGATTTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc     <  2:833588/100‑1 (MQ=255)                                                                                          gCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc     >  1:377077/1‑100 (MQ=255)                                                                                             gATTGCCGTTGCCCAGAACTGCGCATTCTATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAt  <  2:380630/100‑1 (MQ=255)                                                                                                  |                                                                                               TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAT  >  NC_000913/400117‑400308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑