| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 459,256 | T→C | D123D (GAT→GAC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 459,256 | 0 | T | C | 98.2% | 194.4 / ‑4.2 | 56 | D123D (GAT→GAC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (28/27); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
CGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGT > NC_000913/459163‑459350 | cGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCgg > 2:982278/1‑100 (MQ=255) gaggagTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCggg < 1:211484/100‑1 (MQ=255) gggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAAc > 2:1324347/1‑100 (MQ=255) ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACa < 2:840128/100‑1 (MQ=255) gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACGCTTTTCTGCGAAGGCGGAGTAGCTGGAGGCGCCGACCATTGACGAGCGGGAACAg < 2:1375396/100‑1 (MQ=255) gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAg > 2:603207/1‑100 (MQ=255) gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAg < 2:531680/100‑1 (MQ=255) aCAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGCGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGaa > 2:1388637/1‑100 (MQ=255) cAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAg < 1:233817/100‑1 (MQ=255) cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGc > 2:1126641/1‑100 (MQ=255) cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGc > 2:1507596/1‑100 (MQ=255) cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGt > 2:313293/1‑100 (MQ=255) gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg > 1:737722/1‑100 (MQ=255) gTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCg < 1:798026/100‑1 (MQ=255) gTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCg < 1:882331/100‑1 (MQ=255) aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTa > 2:787157/1‑100 (MQ=255) tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc > 1:1536095/1‑100 (MQ=255) tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc < 2:1031606/100‑1 (MQ=255) tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc > 2:1288685/1‑100 (MQ=255) tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTg < 1:817920/100‑1 (MQ=255) tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTg < 1:982284/100‑1 (MQ=255) cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc > 2:1328733/1‑100 (MQ=255) gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa > 1:828863/1‑100 (MQ=255) gcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATc < 1:624599/100‑1 (MQ=255) ctctctGACAATGGCGAACACTTTTCTGCGATGGCGGAGTATCTGTAGTCGCCGACCATTGGCGAGCGGGAACAGGATGTGCTGGTGCGTGCTGCAATCa > 2:1568043/1‑100 (MQ=255) ctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGc > 1:731649/1‑100 (MQ=255) tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc < 1:313315/100‑1 (MQ=255) ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCa > 1:1470365/1‑100 (MQ=255) tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg < 2:49941/100‑1 (MQ=255) ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg < 1:270437/100‑1 (MQ=255) gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAgg > 1:712322/1‑100 (MQ=255) cGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc > 2:143218/1‑100 (MQ=255) acacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTAc < 1:105954/100‑1 (MQ=255) cacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACa < 2:985422/100‑1 (MQ=255) cacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACa < 2:1295279/100‑1 (MQ=255) cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc > 2:798176/1‑100 (MQ=255) cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc > 1:1292331/1‑100 (MQ=255) ttttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCa > 1:885571/1‑100 (MQ=255) tttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAAACAGCCAGTTCGAAGGCTACATCaa < 2:633244/100‑1 (MQ=255) cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt < 1:1290666/100‑1 (MQ=255) gCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGa < 2:1473754/100‑1 (MQ=255) aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc < 1:187883/100‑1 (MQ=255) aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc < 1:275453/100‑1 (MQ=255) tCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTGGAAGGGTACATCAAGCTGAACAAAAGAAGccc > 2:1389356/1‑99 (MQ=255) tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc > 2:170577/1‑100 (MQ=255) ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacca < 1:769689/100‑1 (MQ=255) gAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAg < 1:170577/100‑1 (MQ=255) tCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAg < 1:313293/100‑1 (MQ=255) cGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGt > 2:1089100/1‑100 (MQ=255) ccGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGc < 1:24031/100‑1 (MQ=255) ccGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGc > 1:664381/1‑100 (MQ=255) gACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTg < 1:1424995/100‑1 (MQ=255) gACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTg < 1:6882/100‑1 (MQ=255) ccATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc > 1:1194273/1‑100 (MQ=255) caaTTGACGAGCGAGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc > 1:1066425/3‑100 (MQ=255) aTTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGt > 1:284063/1‑100 (MQ=255) | CGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGT > NC_000913/459163‑459350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |