| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 459,642 | A→G | E252G (GAA→GGA) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 459,642 | 0 | A | G | 95.2% | 197.7 / ‑1.2 | 63 | E252G (GAA→GGA) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (26/34); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGA > NC_000913/459546‑459737 | aGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGc < 1:138176/100‑1 (MQ=255) gATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGcc > 2:229213/1‑100 (MQ=255) aTGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGccc > 2:1126173/1‑100 (MQ=255) tGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGTTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCt > 1:1550323/1‑100 (MQ=255) tGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCt < 1:716605/100‑1 (MQ=255) gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGa > 1:1014767/1‑100 (MQ=255) agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGaa > 2:1275863/1‑100 (MQ=255) aaTCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAgcg < 2:358694/100‑1 (MQ=255) aTCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAgcgc > 2:207631/1‑100 (MQ=255) tCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCa > 2:7113/1‑100 (MQ=255) gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATc < 1:732894/100‑1 (MQ=255) gTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGa < 2:1530800/100‑1 (MQ=255) gAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGAcg < 2:263140/100‑1 (MQ=255) aGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGAcgc > 1:1283650/1‑100 (MQ=255) gTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGAcgcg > 2:1286965/1‑100 (MQ=255) aCTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACgcggc > 2:770643/1‑100 (MQ=255) aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACGCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGaa > 1:1115421/1‑100 (MQ=255) tCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAg < 1:229213/100‑1 (MQ=255) ntGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGC‑AAATCGACGCGGCGAAGAt > 2:538474/2‑100 (MQ=255) cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGa > 1:1530319/1‑100 (MQ=255) cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGa > 1:510654/1‑100 (MQ=255) gAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg > 1:163561/1‑100 (MQ=255) gAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg > 1:214988/1‑100 (MQ=255) aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGcc < 1:718853/100‑1 (MQ=255) aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa < 1:537309/100‑1 (MQ=255) aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGa > 2:384387/1‑99 (MQ=255) cAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAag < 2:1014761/100‑1 (MQ=255) aaaTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGCGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAaga < 2:864579/100‑1 (MQ=255) aaaTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAaga < 1:1399849/100‑1 (MQ=255) aaaTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAaga > 1:75204/1‑100 (MQ=255) aTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg < 1:35073/100‑1 (MQ=255) aaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCaaa < 2:214988/100‑1 (MQ=255) aGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCaaaa > 1:107856/1‑100 (MQ=255) cTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAaga < 1:1442983/100‑1 (MQ=255) tATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAagag < 2:1019685/100‑1 (MQ=255) cAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg > 2:224348/1‑100 (MQ=255) aagaaCTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGa < 2:272757/100‑1 (MQ=255) agaaCTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGaa < 1:1454983/100‑1 (MQ=255) cTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCag < 1:1286970/100‑1 (MQ=255) cGGTGAAATGGACGACGCGCCGGACGAAACCGAAGCCCTGAAGCGCAAAATCCTCGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCagag < 1:1401273/100‑1 (MQ=255) cGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCagag > 2:1190449/1‑100 (MQ=255) tGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTg < 1:7113/100‑1 (MQ=255) gAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGACAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGc > 2:737323/1‑100 (MQ=255) gAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTgt < 1:211144/100‑2 (MQ=255) gAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGc < 2:70266/100‑1 (MQ=255) aaaTGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCa < 1:1487171/100‑1 (MQ=255) gacgacGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGc < 1:715309/100‑1 (MQ=255) gacgacGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGc < 1:1313616/100‑1 (MQ=255) acgacgCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCt < 1:1026040/100‑1 (MQ=255) acgCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGATGCAGAGTTGCAGAAGCTGaa < 2:965096/100‑1 (MQ=255) cgcCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGaa < 2:997377/97‑1 (MQ=255) cGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:784429/100‑1 (MQ=255) ggACGAAAACGGGGCCCTGAAGCGCAAAATCGACGCGGGGAAGATGCCGAAAGTGGCAAAAGAGAAAGCGGAGGCAGTGTTGCAGAAGCTGAAAatgatg < 2:1254007/100‑1 (MQ=255) ggACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgatg > 1:457104/1‑100 (MQ=255) aCGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtc < 1:207631/100‑1 (MQ=255) ngAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtct > 2:481963/2‑100 (MQ=255) cGAAAACGGAGCCCTGAAGCGCAAACTCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtct > 1:1198409/1‑100 (MQ=255) cGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtct > 1:1414367/1‑100 (MQ=255) aaaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTcc < 2:1530310/100‑1 (MQ=255) aaaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAAAGATGtct > 2:1242307/1‑98 (MQ=255) aaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCg < 2:152828/100‑1 (MQ=255) aaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCg < 2:1283645/100‑1 (MQ=255) aaCGGAGCCCTGAAGCGCAAAATCGAAGCGGCGAAGAGGGCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGa < 1:1523518/100‑1 (MQ=255) | AGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGA > NC_000913/459546‑459737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |