Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,805	A→T	C264S (TGC→AGC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,805	0	A	T	100.0%	163.2 / NA	48	C264S (TGC→AGC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/21);  total (27/21)

GCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCC  >  NC_000913/700710‑700901                                                                                                |                                                                                                 gCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCa                                                                                              >  2:427480/1‑100 (MQ=255)    gtcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAg                                                                                           >  2:868075/1‑100 (MQ=255)       gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                        >  1:1288154/1‑100 (MQ=255)       gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                        >  1:1573164/1‑100 (MQ=255)                       ctctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGtt                                                                        <  2:516296/100‑1 (MQ=255)                       ctctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGtt                                                                        <  1:396771/100‑1 (MQ=255)                           ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc                                                                    <  1:803215/100‑1 (MQ=255)                           ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc                                                                    >  1:556756/1‑100 (MQ=255)                               gCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGc                                                                <  1:1387691/100‑1 (MQ=255)                                  cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGt                                                             <  2:816123/100‑1 (MQ=255)                                  cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGt                                                             <  2:1092952/100‑1 (MQ=255)                                     cTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCg                                                          <  2:1247010/100‑1 (MQ=255)                                       ttAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAc                                                        >  1:936870/1‑100 (MQ=255)                                        tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                       >  2:1448479/1‑100 (MQ=255)                                         aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                      >  1:608846/1‑100 (MQ=255)                                          aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                                     <  2:985511/100‑1 (MQ=255)                                             gATTCACCACCCGTTGTTCAATGGCAGCGTTGGCAGCGTTAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                                  <  2:1340226/100‑1 (MQ=255)                                             gATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                                  >  1:1258810/1‑100 (MQ=255)                                                tCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATg                                               >  2:449786/1‑100 (MQ=255)                                                 cAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATgg                                              >  1:1299629/1‑100 (MQ=255)                                                     acCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCaa                                          >  2:375674/1‑100 (MQ=255)                                                         gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTc                                      <  1:1242921/100‑1 (MQ=255)                                                            gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                   <  2:868629/100‑1 (MQ=255)                                                            gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                   <  2:355338/100‑1 (MQ=255)                                                             ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCGGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  1:1363292/1‑100 (MQ=255)                                                             ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  2:556694/1‑100 (MQ=255)                                                             ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  1:1433001/1‑100 (MQ=255)                                                              tCAATGGTAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  <  1:1428585/99‑1 (MQ=255)                                                              tCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  2:90276/1‑99 (MQ=255)                                                               cAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGa                                 >  2:545210/1‑99 (MQ=255)                                                               cAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAc                                >  2:1512282/1‑100 (MQ=255)                                                                aaTGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcg                               >  2:515229/1‑100 (MQ=255)                                                                    gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCGAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc                           >  1:377717/1‑100 (MQ=255)                                                                     cAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCGGCAGTGGCAGCGGTCACCCAGCGGTGCGACCTGAATATGGCCAAGTTCACCGAcgttg                            >  2:935708/1‑98 (MQ=255)                                                                      aGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg                         <  2:640287/100‑1 (MQ=255)                                                                      aGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg                         <  1:556695/100‑1 (MQ=255)                                                                       gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgt                        <  1:427481/100‑1 (MQ=255)                                                                         gTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgtcgccgttg                      <  1:829287/100‑1 (MQ=255)                                                                            ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                   >  2:44093/1‑100 (MQ=255)                                                                            ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                   >  2:936070/1‑100 (MQ=255)                                                                                gCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCaa               >  1:187548/1‑100 (MQ=255)                                                                                    tAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaa           >  2:42548/1‑100 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg       <  2:1079584/100‑1 (MQ=255)                                                                                        ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg       >  1:169244/1‑100 (MQ=255)                                                                                         tCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgc      <  1:1448487/100‑1 (MQ=255)                                                                                            aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc   <  1:1199798/100‑1 (MQ=255)                                                                                            aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc   <  1:1199104/100‑1 (MQ=255)                                                                                             ggCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGTGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGccc  >  2:581420/1‑100 (MQ=255)                                                                                                |                                                                                                 GCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCC  >  NC_000913/700710‑700901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑