Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,209	T→G	intergenic (+380/+356)	ycdU → / ← serX	putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,209	0	T	G	96.2%	63.5 / NA	26	intergenic (+380/+356)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (11/14);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCG  >  NC_000913/1097112‑1097252                                                                                                  |                                            tGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGTTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGa                                           <  2:31292/100‑1 (MQ=255)    cGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggg                                        <  2:622931/100‑1 (MQ=255)     gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAgggg                                       >  1:1365265/1‑100 (MQ=255)     gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAgggg                                       <  1:905122/100‑1 (MQ=255)      aCCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggggg                                      <  1:1330268/100‑1 (MQ=25)        ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggggggg                                    <  2:1365258/100‑1 (MQ=18)          ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAt                                  <  1:445625/100‑1 (MQ=18)          ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAt                                  <  1:506927/100‑1 (MQ=17)           cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAtt                                 <  1:1579345/100‑1 (MQ=18)                ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGAc                              <  1:839160/98‑1 (MQ=17)                ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtc                            >  1:1218853/1‑100 (MQ=18)                 gggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcg                           >  1:151563/1‑100 (MQ=18)                 gggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcg                           >  1:151581/1‑100 (MQ=18)                    cTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgctt                        <  1:775927/100‑1 (MQ=18)                       ctcATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCTTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttc                       <  1:1465074/98‑1 (MQ=255)                        tcATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttcgct                    >  2:680212/1‑100 (MQ=18)                            ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGGTCGcc                >  1:916462/1‑100 (MQ=255)                            ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGcc                >  2:1542775/1‑100 (MQ=18)                            ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGcc                >  2:16676/1‑100 (MQ=18)                              ccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCt              >  1:142582/1‑100 (MQ=18)                                  ggTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCgg          <  1:271093/100‑1 (MQ=17)                                   gtgtgtGCAATACACTAAAAAAAAGCCCGTACTTTCGTACGGGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCggg         <  1:604363/100‑1 (MQ=255)                                    cgggtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGc        <  2:105879/97‑1 (MQ=12)                                      tgtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAg      >  2:720480/1‑100 (MQ=18)                                         gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCgg          >  2:1497575/1‑93 (MQ=33)                                          cAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCg  >  1:525786/1‑100 (MQ=18)                                                                                                  |                                            TGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCG  >  NC_000913/1097112‑1097252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑