Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	162,973	G→A	C290Y (TGT→TAT)	hrpB →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	162,973	0	G	A	100.0%	229.8 / NA	66	C290Y (TGT→TAT)	hrpB	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/26);  total (40/26)

ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGC  >  NC_000913/162876‑163066                                                                                                  |                                                                                              aTCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtatg                                                                                             <  1:614482/100‑1 (MQ=255)  tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGGTGAAGCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtatg                                                                                             <  2:856573/99‑1 (MQ=255)  tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                            <  1:526568/100‑1 (MQ=255)  tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                            >  2:397938/1‑100 (MQ=255)  tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                            <  2:148808/100‑1 (MQ=255)    ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                          >  1:1466568/1‑100 (MQ=255)    ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                          <  2:1580343/100‑1 (MQ=255)     tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGGCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTGTGGTGGTGGATTATGCCCa                                                                                         >  2:1544840/1‑100 (MQ=255)     tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                         <  2:1027082/100‑1 (MQ=255)           cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                   >  1:935059/1‑100 (MQ=255)              cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtgt                                                                                >  1:1342597/1‑100 (MQ=255)                        gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                      >  2:1440938/1‑100 (MQ=255)                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                     <  2:941691/100‑1 (MQ=255)                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                     <  2:827709/100‑1 (MQ=255)                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                     >  2:1320476/1‑100 (MQ=255)                          gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                    <  1:639943/100‑1 (MQ=255)                                tggtgCTGGCGAACAATATTGCTGAATCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                              <  2:1404423/100‑1 (MQ=255)                                  gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                            <  1:795199/100‑1 (MQ=255)                                   tgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCAc                                                           >  1:812145/1‑100 (MQ=255)                                    gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACg                                                          >  2:1563839/1‑100 (MQ=255)                                     cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgg                                                         <  1:1440946/100‑1 (MQ=255)                                     cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgg                                                         <  1:68052/100‑1 (MQ=255)                                      tGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggg                                                        >  2:1406369/1‑100 (MQ=255)                                       ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                       >  2:514193/1‑100 (MQ=255)                                       ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                       >  1:593406/1‑100 (MQ=255)                                        gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                      <  1:873784/100‑1 (MQ=255)                                         cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                     >  2:1436212/1‑100 (MQ=255)                                          gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                    >  1:176120/1‑100 (MQ=255)                                          gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                    >  1:263700/1‑100 (MQ=255)                                            ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAc                                                  <  1:108693/100‑1 (MQ=255)                                            ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAc                                                  >  2:1071308/1‑100 (MQ=255)                                            ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAc                                                  >  1:1406937/1‑100 (MQ=255)                                             cAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcg                                                 >  2:194434/1‑100 (MQ=255)                                              aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                >  2:164463/1‑100 (MQ=255)                                              aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                <  2:1561543/100‑1 (MQ=255)                                               atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                               <  1:373141/100‑1 (MQ=255)                                                tatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGa                                              >  1:228736/1‑100 (MQ=255)                                                tatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGa                                              >  1:372571/1‑100 (MQ=255)                                                 atTGCTGAAACCTGTTTAACCATTGAAGGTATTTGTCTGGTGGTGGATTATGCCCAGGAGTGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                             <  2:32133/100‑1 (MQ=255)                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  2:939390/1‑100 (MQ=255)                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  1:424862/1‑100 (MQ=255)                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  2:859198/1‑100 (MQ=255)                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         <  2:115117/100‑1 (MQ=255)                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  1:152224/1‑100 (MQ=255)                                                        aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                      <  2:1062645/100‑1 (MQ=255)                                                         aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     >  1:1075657/1‑100 (MQ=255)                                                           ccAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa                                   >  2:874467/1‑100 (MQ=255)                                                             aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                 >  1:836255/1‑100 (MQ=255)                                                                ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                              >  2:789964/1‑100 (MQ=255)                                                                  aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                            <  2:520188/100‑1 (MQ=255)                                                                  aaCCATTGAAGGTATACGACTGGTGGTGGATTATGCCCAGAAGCGTGTAGCGAGTTTTGATCCGCGCACGAGGCTTACGCGAATAAATAATCAACGCGtt                            >  2:374054/1‑100 (MQ=255)                                                                     cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                         >  1:931579/1‑100 (MQ=255)                                                                      aTTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGTGCTTACGCGACTGATTACTCAACGCGTTAGcc                        <  2:322392/100‑1 (MQ=255)                                                                         gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                     >  2:190659/1‑100 (MQ=255)                                                                         gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                     >  2:10392/1‑100 (MQ=255)                                                                           aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                   <  1:235421/100‑1 (MQ=255)                                                                           aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                   <  1:1254475/100‑1 (MQ=255)                                                                            ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt                  >  1:815960/1‑100 (MQ=255)                                                                              tATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                >  2:205739/1‑100 (MQ=255)                                                                               aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa               <  1:514194/100‑1 (MQ=255)                                                                                  cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa            <  2:793922/100‑1 (MQ=255)                                                                                   gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc           <  1:855166/100‑1 (MQ=255)                                                                                      tggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa        >  1:195466/1‑100 (MQ=255)                                                                                       ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg       >  2:1477581/1‑100 (MQ=255)                                                                                          ggtggATTATGCCCAGGAGCGTGTGGCGCGATTGGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAAGACGCAGCGt    >  2:1038879/1‑100 (MQ=255)                                                                                           gtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTg   >  2:1377845/1‑100 (MQ=255)                                                                                            tggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc  <  1:1461918/100‑1 (MQ=255)                                                                                            tggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc  >  1:657393/1‑100 (MQ=255)                                                                                                  |                                                                                              ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGC  >  NC_000913/162876‑163066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑