| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,197,739 | A→G | S167P (TCC→CCC) | ymfE ← | e14 prophage; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,197,739 | 0 | A | G | 96.9% | 209.3 / ‑2.2 | 65 | S167P (TCC→CCC) | ymfE | e14 prophage; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (35/28); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01 | |||||||||||
CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTT > NC_000913/1197643‑1197833 | ctGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAAt > 1:974103/1‑100 (MQ=255)ctGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATACATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAAt > 1:118592/1‑100 (MQ=255) tGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAAtt > 2:1276269/1‑100 (MQ=255) gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAAt < 2:854865/100‑1 (MQ=255) ataaTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGc < 2:427122/97‑1 (MQ=255) aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGc < 1:1052729/100‑1 (MQ=255) tGATAAAATTACAAAAAAGAGTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCa > 2:969082/1‑100 (MQ=255) gATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCaa < 1:339872/100‑1 (MQ=255) aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAAt < 1:886281/100‑1 (MQ=255) aTAAAATAACAAAAAAGAATAAAGACAGAAAAGAAACTTCAACATGAAAAATATAAAAAAAAAAAA‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAAt < 2:388986/100‑1 (MQ=255) aaaaTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTa < 2:368687/100‑1 (MQ=255) aaTTACAAAAAAGAATAAATACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATc < 2:567987/100‑1 (MQ=255) aTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCg > 1:1097617/1‑100 (MQ=255) aCAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaa > 2:1396344/1‑100 (MQ=255) cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa < 1:1019712/100‑1 (MQ=255) cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa > 1:376387/1‑100 (MQ=255) aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAt < 1:210662/100‑1 (MQ=255) aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt > 2:500498/1‑100 (MQ=255) aaaaaaATAAAAACAGCAAAGAACCTTCAACATGAAAAATATCCAAATAATTAA‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt < 2:1390520/99‑1 (MQ=255) aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc > 2:476505/1‑100 (MQ=255) aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc < 2:504287/100‑1 (MQ=255) aaaaGAATAAAGACAGCAAAGAAACTTCAACATGCAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc < 2:834735/100‑1 (MQ=255) aaaGAATAACGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGATAATTCa < 1:456878/100‑1 (MQ=255) gAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGTGAATTAATGCAATTATCGAAAATTCaaaa > 2:70666/1‑100 (MQ=255) aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGCGTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa > 2:1499843/1‑100 (MQ=255) aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaca > 1:21107/1‑98 (MQ=255) aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa > 1:1299032/1‑100 (MQ=255) tAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAat > 1:583854/1‑100 (MQ=255) aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata < 1:227097/100‑1 (MQ=255) aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata > 2:1019301/1‑100 (MQ=255) cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa < 2:478830/100‑1 (MQ=255) gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa > 1:250016/1‑100 (MQ=255) cAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa > 1:1535242/1‑100 (MQ=255) aaaGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAAt > 2:1272337/1‑100 (MQ=255) aaGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa < 1:463453/100‑1 (MQ=255) aGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAg < 1:1341640/100‑1 (MQ=255) gAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGt < 2:144423/100‑1 (MQ=255) tGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATACAAAAAATATCCAAAAATAGTATACTTTATTcc < 2:271464/100‑1 (MQ=255) gAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa > 2:872878/1‑100 (MQ=255) gAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa > 1:473557/1‑100 (MQ=255) aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag > 2:1029650/1‑100 (MQ=255) aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag < 1:278755/100‑1 (MQ=255) aaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag < 1:1243691/100‑1 (MQ=255) tatCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaaga > 2:348169/1‑99 (MQ=255) cATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCa > 2:1366376/1‑100 (MQ=255) cATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATCATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAAAAGAGTTCa > 1:1031298/1‑100 (MQ=255) tttgtttgCAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa < 2:1172394/100‑1 (MQ=255) aaaaaaaaaaaaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa < 2:1491840/90‑1 (MQ=255) gtttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAtata > 1:491196/1‑100 (MQ=255) tgC‑AAAAAAAGATTATTAGGTAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATg > 2:661594/1‑100 (MQ=255) gC‑AAAAAAAGATTATTAGGAAGGGAATTTATGCAATTATCGAATATTCAAACAATATCTTAAAATATTACACTTTATTCCAGAAGATTCCAATATAAt > 2:1492622/1‑98 (MQ=255) gC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt > 2:266289/1‑100 (MQ=255) gC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt > 2:861079/1‑100 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt > 2:340954/1‑100 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt > 1:1004581/1‑100 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt > 1:1387669/1‑100 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt < 2:1103572/100‑1 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt > 2:469766/1‑100 (MQ=255) c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAAAGTTCAATATAATGtt > 2:112653/1‑100 (MQ=255) aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg < 1:1276274/100‑1 (MQ=255) aaaaaaGATTATTAAAAAGGGAATTAATGCAATTATCAAAAATAAAAAAAATATCAAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg < 2:725409/100‑1 (MQ=255) attattAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCaa > 2:1215733/1‑100 (MQ=255) attattAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCaa > 1:871291/1‑100 (MQ=255) taGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCATAAGAGTTCAATATAATGTTTGTCTTCAAttttt < 2:486506/100‑1 (MQ=255) taGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAttttt > 1:770194/1‑100 (MQ=255) aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc < 2:1229295/100‑1 (MQ=255) aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc > 2:688211/1‑100 (MQ=255) aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc < 1:688213/100‑1 (MQ=255) gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt < 1:1274417/100‑1 (MQ=255) gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt < 2:408907/100‑1 (MQ=255) gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt < 2:408159/100‑1 (MQ=255) gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt < 1:1362072/100‑1 (MQ=255) | CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTT > NC_000913/1197643‑1197833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |