Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,902	C→T	L84L (TTG→TTA)	mtn ←	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,902	0	C	T	97.0%	214.0 / ‑1.7	66	L84L (TTG→TTA)	mtn	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (33/31);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

ACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCG  >  NC_000913/178809‑178995                                                                                              |                                                                                              aCAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGtt                                                                                         <  2:816367/100‑1 (MQ=255) aCAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGtt                                                                                         <  1:1496057/100‑1 (MQ=255)  cAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTg                                                                                        <  1:902216/100‑1 (MQ=255)    gCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGt                                                                                      >  1:227115/1‑100 (MQ=255)    gCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGt                                                                                      >  1:1501805/1‑100 (MQ=255)     ccTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTg                                                                                     <  1:1273308/100‑1 (MQ=255)     ccTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTg                                                                                     <  2:369324/100‑1 (MQ=255)     ccTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTg                                                                                     <  2:1222949/100‑1 (MQ=255)       tGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGcc                                                                                   <  1:1194967/100‑1 (MQ=255)              tGACCGTATTCATAACCTAATGCCCTGACATCCGCGTCGTGATAACGTGCTTCGTCTGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAAGCCAc                                                                            >  2:1001421/1‑100 (MQ=255)                 ccGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCgg                                                                         >  2:893768/1‑100 (MQ=255)                  cGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGc                                                                        <  1:794475/100‑1 (MQ=255)                  cGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGc                                                                        <  1:1196649/100‑1 (MQ=255)                   gTATTCACAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCa                                                                       <  2:939649/100‑1 (MQ=255)                     aTTCATAACCAAATGCCGTGACATCCGCGTCTTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGa                                                                     <  2:268621/100‑1 (MQ=255)                       tCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAAc                                                                   >  2:177712/1‑100 (MQ=255)                        cATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                  >  1:222531/1‑100 (MQ=255)                        cATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                  >  1:253402/1‑100 (MQ=255)                        cATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                  >  2:1131197/1‑100 (MQ=255)                          tAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACATTGGTGCCAGGCCACCGGCAGAACCg                                                                 >  2:218238/1‑99 (MQ=255)                            aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                >  1:667602/1‑98 (MQ=255)                            aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCg                                                                 >  2:1236320/1‑97 (MQ=255)                            aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGgtg                                                              <  1:269538/100‑1 (MQ=255)                             ccAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGgtgt                                                             <  1:946745/100‑1 (MQ=255)                               aaaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTta                                                           <  1:803177/100‑1 (MQ=255)                                 aTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaat                                                         <  1:515265/100‑1 (MQ=255)                                   gCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaataa                                                       <  2:1397130/100‑1 (MQ=255)                                      gTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATca                                                    <  1:802099/100‑1 (MQ=255)                                           aTCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCt                                               >  1:1195948/1‑100 (MQ=255)                                             ccGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTgg                                             >  1:1343774/1‑100 (MQ=255)                                              cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGc                                            <  1:1155433/100‑1 (MQ=255)                                                  tcgtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                        <  1:503490/100‑1 (MQ=255)                                                  tcgtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                        <  2:8010/100‑1 (MQ=255)                                                  tcgtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                        >  1:666106/1‑100 (MQ=255)                                                    gtGATAACGTGCTTCGTCCGAGACAACGATATCACCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAg                                      >  1:361910/1‑100 (MQ=255)                                                         taCGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGTCAAGCCACCGGCCGAACCGGTGTTGATAATCACATCTGGCTCGCAgtgt                                   >  2:1284596/2‑98 (MQ=255)                                                          ncGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTcc                                >  2:245734/2‑100 (MQ=255)                                                          aCGTGCTTCGTCCGAGACAACGCTATCGCCCACTTTTAACTTTGGTGTCAGGCCACCGACAGAACCGGTCTTAGTAATCACATCGGGCATGCCGTGtt                                  >  2:881815/1‑98 (MQ=255)                                                          aCGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTcc                                <  2:336686/100‑1 (MQ=255)                                                           cGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATTATCACATCTGGCTTGCAGTGtt                                  <  2:915586/97‑1 (MQ=255)                                                           cGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCa                               >  2:1148336/1‑100 (MQ=255)                                                           cGTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCa                               >  1:921819/1‑100 (MQ=255)                                                            gTGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATCATCACATCTGGCTTGCAGTGTTCCaa                              <  1:960226/100‑1 (MQ=255)                                                            gTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCaa                              >  1:858591/1‑100 (MQ=255)                                                            gTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCaa                              >  1:858569/1‑100 (MQ=255)                                                             tGCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc                             <  1:1479305/100‑1 (MQ=255)                                                              gCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACa                            >  1:1193601/1‑100 (MQ=255)                                                              gCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACa                            <  1:1407577/100‑1 (MQ=255)                                                              gCTTCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACa                            <  2:1543972/100‑1 (MQ=255)                                                                ttCGTCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGc                          >  1:1537386/1‑100 (MQ=255)                                                                   ntCCGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCaaa                       >  1:14519/2‑100 (MQ=255)                                                                   gTCCGAGACAACGATATCGCCCCCTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCaaa                       >  2:1328451/1‑100 (MQ=255)                                                                      cGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGACCCGGTGTTAATAATCACATCTGGCTTGCAGAGTTCCAACAGCAAAGTg                    >  1:1577001/1‑100 (MQ=255)                                                                      cGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                    >  1:1107203/1‑100 (MQ=255)                                                                      cGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                    >  1:1575497/1‑100 (MQ=255)                                                                      cGAGACAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                    >  2:1334030/1‑100 (MQ=255)                                                                       gagaCAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTgg                   >  2:1546526/1‑100 (MQ=255)                                                                       gagaCAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTgg                   >  2:1214095/1‑100 (MQ=255)                                                                        agaCAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGc                  >  2:727278/1‑100 (MQ=255)                                                                           cAACGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACGGGTGTTAATAATAACATGTGGCTTGCAGTGTTCCAACAGCAAAGTGGCAc                >  2:1210593/1‑99 (MQ=255)                                                                            aaCGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCAccc              <  2:666104/100‑1 (MQ=255)                                                                            aaCGATATCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCAccc              <  1:177712/100‑1 (MQ=255)                                                                                atatCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAgcg          <  2:795838/100‑1 (MQ=255)                                                                                 tatCGCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAgcgc         <  1:1406734/100‑1 (MQ=255)                                                                                     gCCCACTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCa     >  1:32157/1‑100 (MQ=255)                                                                                         aCTTTTAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCg  <  1:427962/99‑1 (MQ=255)                                                                                              |                                                                                              ACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCG  >  NC_000913/178809‑178995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑