| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,547,395 | A→G | intergenic (‑226/‑6) | yddG ← / → fdnG | aromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,547,395 | 0 | A | G | 92.5% | 161.7 / 1.0 | 54 | intergenic (‑226/‑6) | yddG/fdnG | aromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (23/26); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAA > NC_000913/1547303‑1547490 | gtgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAAt > 2:517166/1‑100 (MQ=255) tgGCAAACAGATGTTCTTTTTTATTTCGTGCAAAAAGAATCAGAATTTTACTGTTAGTTTCCTCGCCCAGTTATACCCCTGAGTAATGTCGAAGGCAATg > 2:124345/1‑100 (MQ=255) tgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATg > 2:330519/1‑100 (MQ=255) ggCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATgg > 2:1228895/1‑100 (MQ=255) cAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGAc < 2:898948/100‑1 (MQ=255) taaCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACg < 1:25157/99‑1 (MQ=255) aaCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACga > 2:25157/1‑99 (MQ=255) tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCg > 2:582729/1‑100 (MQ=255) ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCa < 1:826237/100‑1 (MQ=255) cTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGa > 1:647467/1‑100 (MQ=255) tttttGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGAc < 2:19841/100‑1 (MQ=255) aTTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttt > 1:710696/1‑100 (MQ=255) tttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGTGAAGCAATGGACGTCAGTCGCAGACAAttttt < 1:1498817/100‑1 (MQ=255) ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt < 2:746929/100‑1 (MQ=255) ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGGAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt < 2:90847/100‑1 (MQ=255) cgcgCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaa < 1:529560/100‑1 (MQ=255) cgcgCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaa < 1:206206/100‑1 (MQ=255) cgcgCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaa > 2:365574/1‑100 (MQ=255) gcggAAAAAGATTCAGAATGTTACTGTTAGTGTCCTCGCGCAGTAATACCCCTCAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt < 2:1276158/97‑1 (MQ=255) gcgcAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaaa < 1:1083507/100‑1 (MQ=255) cgcAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaaaa > 1:720449/1‑100 (MQ=255) cAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATc > 2:835156/1‑100 (MQ=255) cAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATc > 2:1571304/1‑100 (MQ=255) aaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGTAAGCAATGGATGTCAGTCGCAGACAATTTTTTAAAATCTgtg < 1:1066517/100‑3 (MQ=255) aaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcg > 1:582490/1‑100 (MQ=255) ttCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcggg > 1:122421/1‑100 (MQ=255) cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg < 1:1473420/100‑1 (MQ=255) aTTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTAt < 2:763953/100‑1 (MQ=255) aTTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTAt < 2:622532/100‑1 (MQ=255) taaCTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGGAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTAGGGc > 1:1463918/3‑100 (MQ=255) cTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTgg < 2:51949/100‑1 (MQ=255) cTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTgg < 2:1463464/100‑1 (MQ=255) tGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGa < 1:822611/100‑1 (MQ=255) gTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaa > 1:246435/1‑100 (MQ=255) ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac < 1:242582/100‑1 (MQ=255) gTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaac < 1:365574/100‑1 (MQ=255) tttCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAacaaca < 1:321656/100‑1 (MQ=255) tttCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAacaaca < 1:138700/100‑1 (MQ=255) gcgcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCgg < 1:253226/100‑1 (MQ=255) gcgcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCgg < 2:190644/100‑1 (MQ=255) gcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCa < 1:491446/100‑1 (MQ=255) cAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCAt > 1:1297830/1‑100 (MQ=255) gTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTg > 1:986998/1‑100 (MQ=255) tAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTgg > 1:269137/1‑100 (MQ=255) tACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCt < 2:828831/100‑1 (MQ=255) tACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCt < 1:835161/100‑1 (MQ=255) ccccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt > 2:1195602/1‑100 (MQ=255) ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc > 2:190977/1‑100 (MQ=255) cTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcc > 1:84278/1‑100 (MQ=255) tGAAAAAAGAGGGAAGCAACGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGccc < 1:709655/100‑1 (MQ=255) gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc < 1:1312508/100‑1 (MQ=255) aaaaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCg > 1:1131888/1‑100 (MQ=255) agagGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACGACAGTAGCGGCATTGGGCTTTGCCCCGaagca < 1:124345/100‑1 (MQ=255) gagGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagcaa > 1:1453239/1‑100 (MQ=255) | GTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAA > NC_000913/1547303‑1547490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |