| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,555,324 | A→G | S116P (TCT→CCT) | maeA ← | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,555,324 | 0 | A | G | 97.7% | 147.1 / ‑4.3 | 44 | S116P (TCT→CCT) | maeA | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (28/15); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
TATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT > NC_000913/1555231‑1555420 | tattatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACg > 1:315410/1‑100 (MQ=255) gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTATGGTAAGAGATAAACACGCCGCGTGAAAGGCGGTAGATCTCAGGAAAACGCTCACAg < 2:921811/100‑1 (MQ=255) ttCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAgg > 1:1508438/1‑100 (MQ=255) tCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGc > 1:1549550/1‑100 (MQ=255) aCGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgcgc > 1:112620/1‑100 (MQ=255) gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCg > 2:378849/1‑100 (MQ=255) ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg > 1:624258/1‑100 (MQ=255) gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGAGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGt > 1:438643/1‑100 (MQ=255) aaTATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg > 1:381984/1‑100 (MQ=255) atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTgggg < 2:149947/100‑1 (MQ=255) atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta > 1:16077/1‑100 (MQ=255) cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata > 2:1460015/1‑100 (MQ=255) tCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaa < 2:427664/100‑1 (MQ=255) tatTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAAc > 1:598877/1‑100 (MQ=255) gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc > 1:134387/1‑100 (MQ=255) tgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGca > 1:1225898/1‑100 (MQ=255) gCCGGTTCTGGTCAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcat > 2:87617/1‑100 (MQ=255) gCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcat > 2:1052079/1‑100 (MQ=255) ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct > 1:898035/1‑100 (MQ=255) tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat > 1:197221/1‑100 (MQ=255) tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat > 1:1168949/1‑100 (MQ=255) ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc < 1:119752/100‑1 (MQ=255) gTAAGAGTTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGATACCGCTCACTGGCTGCGCCGACGGTTGGGGTATAAATTACAGGCATCATCtcatca < 1:929433/100‑1 (MQ=255) tAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCaa > 2:1141390/1‑100 (MQ=255) aaGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg > 1:1288658/1‑100 (MQ=255) gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCACgat > 2:271015/1‑100 (MQ=255) tccgatctcgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatga < 2:789480/92‑1 (MQ=255) aaaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg > 2:1344468/1‑100 (MQ=255) aaaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg < 2:1473341/100‑1 (MQ=255) acacGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGtt < 2:550588/100‑1 (MQ=255) acGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTa < 2:1225893/100‑1 (MQ=255) cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatgaagatcgga > 1:789485/1‑92 (MQ=255) gcgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGa < 1:24792/100‑1 (MQ=255) gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGt < 2:19189/100‑1 (MQ=255) gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGt < 1:1052743/100‑1 (MQ=255) aCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTag < 2:718957/100‑1 (MQ=255) ggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaa < 1:1485648/100‑1 (MQ=255) ggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaa < 2:1549540/100‑1 (MQ=255) gcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGCTGATTGTTTACCAGACGGTagaag > 1:692334/1‑100 (MQ=255) tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg < 2:982435/100‑1 (MQ=255) aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGttt > 2:174995/1‑100 (MQ=255) aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGttt > 1:1440229/1‑100 (MQ=255) aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGACTAAATAACAGTCATCATCTCATCAATGTGATCGTTTAcacac > 2:1336447/1‑80 (MQ=255) cAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt > 2:765088/1‑100 (MQ=255) | TATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT > NC_000913/1555231‑1555420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |