Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,650,904	T→C	pseudogene (60/693 nt)	insD1 →	IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,650,904	0	T	C	100.0%	39.8 / NA	13	pseudogene (60/693 nt)	insD1	IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/7);  total (6/7)

CAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGC  >  NC_000913/1650810‑1650938                                                                                               |                                   cAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTa                               >  2:926155/1‑100 (MQ=255)   gACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTAcc                             >  1:26715/1‑100 (MQ=255)   gACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTAcc                             <  1:922068/100‑1 (MQ=255)      aTGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGa                          >  2:1485189/1‑100 (MQ=255)       tGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGAt                         <  1:676533/100‑1 (MQ=255)       tGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGAt                         <  1:680726/100‑1 (MQ=255)        ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGAt                         <  1:33598/99‑1 (MQ=255)        ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc                        <  2:1332855/100‑1 (MQ=255)        ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc                        >  2:1362310/1‑100 (MQ=255)        ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc                        >  2:364191/1‑100 (MQ=255)        ggAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATc                        <  2:731734/100‑1 (MQ=255)                 tGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAg               <  2:767527/100‑1 (MQ=255)                              ggCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGCGTTTACCGGATCATGCGCCAGAATGCGCTGTTGc  >  1:819081/1‑100 (MQ=18)                                                                                               |                                   CAGACATGGAAAACACTGGATGACGAACTGGCCTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGC  >  NC_000913/1650810‑1650938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑