| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,658,592 | C→T | A175V (GCC→GTC) | ynfE → | putative selenate reductase, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,658,592 | 0 | C | T | 98.0% | 161.6 / NA | 49 | A175V (GCC→GTC) | ynfE | putative selenate reductase, periplasmic |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (28/20); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATG > NC_000913/1658495‑1658680 | cTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCt > 1:243679/1‑100 (MQ=255) gaagaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcg > 2:274418/1‑100 (MQ=255) gaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcggcg > 1:36198/1‑100 (MQ=255) aaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGt > 2:193756/1‑100 (MQ=255) aaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGt < 1:292696/100‑1 (MQ=255) aaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTc < 1:148532/100‑1 (MQ=255) tCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATAGTTGGCCGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACg < 2:680563/100‑1 (MQ=255) cGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGc > 1:722712/1‑100 (MQ=255) cGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGc > 2:751829/1‑100 (MQ=255) gAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGcc > 1:1145399/1‑100 (MQ=255) aaCAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCt > 2:324589/1‑100 (MQ=255) atatGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCtgatg > 1:1435741/1‑100 (MQ=255) atGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGaa > 2:1171637/1‑100 (MQ=255) ggCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACt > 2:435172/1‑100 (MQ=255) cgaTGAGGGTGTAAATAATCCCTTCTCATCGGGGATCAATGAGGGCAATATGACCCGCTCTTCGCCGTCAGTCTCGGCGGTCAAACGCCTGATGAACTg < 2:1300093/97‑1 (MQ=255) tgagtttGTACATATTCACTGCTCATCGGGGATCGTTGGCGGCAGTATATCCCCCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGc < 2:1275514/91‑1 (MQ=255) tGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAc < 1:981114/100‑1 (MQ=255) aGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAcgg > 1:1230439/1‑100 (MQ=255) ggCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAcggc < 2:1188035/100‑1 (MQ=255) gCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAcggcg > 1:584454/1‑100 (MQ=255) tGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGt > 1:506822/1‑100 (MQ=255) atatatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCa > 1:464396/1‑100 (MQ=255) atatatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTc < 2:1120100/99‑1 (MQ=255) tatatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCAc < 2:1230434/100‑1 (MQ=255) atTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCa > 1:784813/1‑100 (MQ=255) atTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCa > 2:343074/1‑100 (MQ=255) cAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAcc > 2:1542547/1‑100 (MQ=255) cAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAcc > 1:1568608/1‑100 (MQ=255) aGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCa < 1:1488533/100‑1 (MQ=255) gTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAg < 2:1164281/100‑1 (MQ=255) gTACCCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAg < 2:1314398/100‑1 (MQ=255) cctgtaccgggATGGTTGGCGGCAATCAGTCCCGCTATTCGCCAGCAGTCTCGGCGGTCAAACGCCGGATGGACTGCTACGGCGGTTCACTCAACCa < 2:329400/89‑1 (MQ=255) aCTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTa < 1:1174042/100‑1 (MQ=255) ctctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTAt > 2:1027534/1‑100 (MQ=255) tctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATg < 1:980076/100‑1 (MQ=255) ttCGGGGATCGTTGGCGGCAATATGCCCCGCTCTTCGCCATGAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGc < 2:10123/100‑1 (MQ=255) tCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAg < 1:324589/100‑1 (MQ=255) ggcggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTg > 1:537389/1‑100 (MQ=255) cggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTgcg > 1:454651/1‑100 (MQ=255) ggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTgcgc < 2:506821/100‑1 (MQ=255) tatGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAAtt > 2:1336001/1‑100 (MQ=255) aCCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTcc > 2:997411/1‑99 (MQ=255) aCCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCt > 1:1434721/1‑100 (MQ=255) ccGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgt > 1:1386531/1‑100 (MQ=255) cGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgtg > 1:671735/1‑100 (MQ=255) cGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgtg < 2:94301/100‑1 (MQ=255) tctTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCa < 2:795966/100‑1 (MQ=255) ctTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCAt > 2:721307/1‑100 (MQ=255) ttCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATg < 1:385339/100‑1 (MQ=255) | CTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATG > NC_000913/1658495‑1658680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |