| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,821,182 | T→A | V11V (GTA→GTT) | chbC ← | N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,821,182 | 0 | T | A | 92.3% | 162.7 / 2.4 | 52 | V11V (GTA→GTT) | chbC | N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base A (23/25); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACG > NC_000913/1821086‑1821281 | ggCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAAcc > 2:873930/1‑100 (MQ=255)ggCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAAcc > 1:3597/1‑100 (MQ=255) cccGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCtttt < 1:1359947/100‑1 (MQ=255) cGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCa > 2:5785/1‑100 (MQ=255) ggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGAt < 1:379528/100‑1 (MQ=255) ggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGAt < 2:1313926/100‑1 (MQ=255) gTTAACGGCATTAAGCGAATAAAGCCATTTTTTTTTTTTTTTACGTGTGGCTGCTTTCCTATTTTAACTGCTTAAGGGAGGAGAACCTTTTCAAGc > 2:202468/1‑96 (MQ=255) gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATg < 1:1266091/100‑1 (MQ=255) gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAAGAGAACCTTTTCAAGCGATg < 2:966015/100‑1 (MQ=255) ttAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGc < 2:1173344/100‑1 (MQ=255) aCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAAt > 2:209754/1‑100 (MQ=255) gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAAc < 2:1488422/100‑1 (MQ=255) aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACt > 1:837444/1‑100 (MQ=255) aGCGAATAAAGCCATTTTTGATTGCACTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACt > 1:960848/1‑100 (MQ=255) cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCa < 2:1401805/100‑1 (MQ=255) cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCa < 2:1400564/100‑1 (MQ=255) aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGaaa > 2:568292/1‑100 (MQ=255) cATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATa > 1:1024866/1‑100 (MQ=255) cATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATa > 2:1098403/1‑100 (MQ=255) aTTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATAc < 1:608544/100‑1 (MQ=255) ttttGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATAccc > 1:9391/1‑100 (MQ=255) tttGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCt < 1:920069/100‑1 (MQ=255) tctttAATGTGTGGCGGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAAc > 2:314386/4‑100 (MQ=255) aTTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCg > 2:672445/1‑100 (MQ=255) ttAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGc > 1:674829/1‑100 (MQ=255) tAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACACTTAAAAACCGCa > 2:570600/1‑100 (MQ=255) tAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCa > 2:41065/1‑100 (MQ=255) aaCGTGTGGCTGCTTTCCTATTTTAACTTCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCa < 2:393339/99‑1 (MQ=255) cGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAtt < 1:587043/100‑1 (MQ=255) gtgtGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAttt > 1:35263/1‑100 (MQ=255) gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAat < 2:1290202/99‑1 (MQ=255) ctgctTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAatat > 1:820383/1‑100 (MQ=255) gctTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTg < 1:914614/100‑1 (MQ=255) ctTTCATATTTTAACTGCAAAAGGCAGGAGTAACTTTTCAAGCGATGCAATAACATTACACATAGAAAAATACCCTTAAAAACCGCAATTTAAATAGTGc < 2:234055/100‑1 (MQ=255) ttCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCgg < 2:455258/100‑1 (MQ=255) ccTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTa > 2:1456115/1‑100 (MQ=255) ccTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTa > 2:120961/1‑100 (MQ=255) aTTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTg < 1:209754/100‑1 (MQ=255) aCTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTAt < 1:744373/100‑1 (MQ=255) aCTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTAt > 1:742262/1‑100 (MQ=255) cTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATg < 1:1337310/100‑1 (MQ=255) tGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGa < 1:1167371/100‑1 (MQ=255) gCAAGAGGGAGGAGAACCTGTTCAAGCGATGCAATAGCATTACGCATAGAGAAATACCCTTAAAAACCGCAATTTAAAGATTGCGGTATTGAttt < 2:63364/95‑1 (MQ=255) gCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaa > 2:696286/1‑100 (MQ=255) cAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaa < 1:630403/100‑1 (MQ=255) agaaGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAAt < 2:960843/98‑1 (MQ=255) aaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa > 2:463658/1‑100 (MQ=255) aaaGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa > 2:1303138/1‑100 (MQ=255) aaaGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa > 2:893738/1‑100 (MQ=255) gggAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAAct < 1:364260/100‑1 (MQ=255) ggagAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCttt < 2:35263/100‑1 (MQ=255) ggagAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCttt > 2:1130097/1‑100 (MQ=255) tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACg < 1:280108/100‑1 (MQ=255) | GGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACG > NC_000913/1821086‑1821281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |