| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,827,031 | T→A | D410V (GAT→GTT) | astB ← | succinylarginine dihydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,827,031 | 0 | T | A | 95.2% | 203.5 / ‑0.6 | 63 | D410V (GAT→GTT) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (31/29); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGC > NC_000913/1826936‑1827125 | cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc > 2:440738/1‑100 (MQ=255) gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc < 1:1140324/100‑1 (MQ=255) cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCCCCCAGCAATTGCGGGCCGGCCCGCACGgcagca > 1:956877/1‑100 (MQ=255) tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag < 1:814743/100‑1 (MQ=255) ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt > 2:1194732/1‑100 (MQ=255) aaCGGATAAACCGAACCGAGCTTCAGTCATTTGCTCAATCAATCCAGTGCTTGCCGCCCTTGGCGCAGCAATTGGGGTTCGGCCAGAACGgccgca > 2:646936/1‑96 (MQ=255) aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTaa > 2:1255683/1‑100 (MQ=255) gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg > 2:1135084/1‑100 (MQ=255) tAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGAt > 2:775698/1‑100 (MQ=255) aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGATCGGCCAGAACGGCAGCAGTAAGGCGATcg > 2:542377/1‑100 (MQ=255) aaccgaaccgAGATTCAGTAATTGGCTCAATACACCCAGTCCTTCCCGCCCTTCGCGCAGCAAGCGCGGGTCGCCCAGAACCGCAGCAGTAAGGc > 2:1050090/1‑95 (MQ=255) accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc > 1:284479/1‑100 (MQ=255) accgaGATTCAGAAATTGGCTCAATACATCCAGCGCTTCCCACCCTTCGCGCAGAAATTGCGGGTCGGCAAGAACGGCAGCAGTAAGGCGATAGTGgta > 2:1518412/1‑99 (MQ=255) ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt > 2:1226108/1‑100 (MQ=255) gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg < 1:66527/100‑1 (MQ=255) gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAc < 1:1342190/100‑1 (MQ=255) gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGGAGTAAGGCGATCGCGGTAGTAACGATCCAc > 1:133602/1‑100 (MQ=255) aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc < 2:1358016/100‑1 (MQ=255) aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc > 1:524908/1‑100 (MQ=255) ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc < 2:794929/100‑1 (MQ=255) gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa > 1:1150347/1‑100 (MQ=255) cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt > 2:1292825/1‑100 (MQ=255) cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt > 1:721372/1‑100 (MQ=255) cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt > 2:217886/1‑100 (MQ=255) cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg < 2:1046/100‑1 (MQ=255) aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg < 2:1136100/100‑1 (MQ=255) aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg < 2:37783/100‑1 (MQ=255) cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc < 2:919877/100‑1 (MQ=255) tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt > 1:918168/1‑100 (MQ=255) tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt > 1:1137817/1‑100 (MQ=255) tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt > 2:870949/1‑100 (MQ=255) ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt > 1:760892/1‑100 (MQ=255) ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt < 1:1255688/100‑1 (MQ=255) ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGCGTCGGCCAGCACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGCGCGCact > 1:1477800/1‑98 (MQ=255) gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaaa > 1:48860/1‑100 (MQ=255) cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg > 1:45645/1‑100 (MQ=255) ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc < 2:551933/100‑1 (MQ=255) cccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt > 2:276292/1‑100 (MQ=255) tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca < 2:1118250/100‑1 (MQ=255) tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca < 1:542378/100‑1 (MQ=255) gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc < 1:1274103/100‑1 (MQ=255) gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc < 2:1419676/100‑1 (MQ=255) cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca < 1:1528816/100‑1 (MQ=255) cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca < 2:615447/100‑1 (MQ=255) cagcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatc < 1:520511/100‑1 (MQ=255) agcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatca < 2:1211230/100‑1 (MQ=255) gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc < 1:440739/100‑1 (MQ=255) aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc < 1:1240398/100‑1 (MQ=255) tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg < 1:351215/100‑1 (MQ=255) gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg < 2:898820/100‑1 (MQ=255) gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg < 1:1135089/100‑1 (MQ=255) gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg < 1:901635/100‑1 (MQ=255) gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg < 1:1094006/100‑1 (MQ=255) ctgggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg < 1:270605/98‑1 (MQ=255) gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGag > 2:270605/1‑99 (MQ=255) gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt > 1:113613/1‑100 (MQ=255) gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc < 1:870954/100‑1 (MQ=255) tCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCa < 2:1000418/100‑1 (MQ=255) cGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAc > 2:1296569/1‑100 (MQ=255) nccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg > 2:732019/2‑100 (MQ=255) gCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg < 1:934812/100‑1 (MQ=255) gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg > 2:1302974/1‑100 (MQ=255) ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc > 1:985979/1‑100 (MQ=255) | CGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGC > NC_000913/1826936‑1827125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |