Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,790	T→G	G180G (GGT→GGG)	yebB →	DUF830 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,790	0	T	G	100.0%	197.3 / NA	54	G180G (GGT→GGG)	yebB	DUF830 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/26);  total (28/26)

ATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAG  >  NC_000913/1946696‑1946884                                                                                               |                                                                                               aTGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGgt                                                                                           <  1:1315813/100‑1 (MQ=255)  tGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGgtg                                                                                          <  1:925091/100‑1 (MQ=255)   gCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGgtgt                                                                                         >  1:785204/1‑100 (MQ=255)    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        <  1:1030114/100‑1 (MQ=255)    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        <  1:842829/100‑1 (MQ=255)    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        >  2:795781/1‑100 (MQ=255)      aaaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGa                                                                                      <  2:798479/100‑1 (MQ=255)      aaaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGa                                                                                      <  1:452722/100‑1 (MQ=255)       aaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGAt                                                                                     <  2:1285995/100‑1 (MQ=255)         cTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTc                                                                                   >  2:148285/1‑100 (MQ=255)         cTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTc                                                                                   <  1:1011636/100‑1 (MQ=255)           cACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCAt                                                                                 >  2:195336/1‑100 (MQ=255)            aCTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATg                                                                                <  1:1121133/100‑1 (MQ=255)             cTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGc                                                                               >  2:556473/1‑100 (MQ=255)               ttCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATg                                                                                <  2:530594/97‑1 (MQ=255)                  tGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCggagg                                                                          <  2:822385/100‑1 (MQ=255)                  tGGAAAGTGTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAATCCGTCGGGCCGGGCAGTTTGTGGCATAGTCCGGGGTTGGTGTTGAt                                                                                     <  2:716388/89‑1 (MQ=255)                    gAAATTCTGGGTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCAGCATCCGGGGTTGGTGTTGATTCATGCggaggga                                                                        <  2:1035623/100‑1 (MQ=255)                      aaTTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGt                                                                      >  2:13946/1‑100 (MQ=255)                         tCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGGTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGa                                                                   <  2:1000134/100‑1 (MQ=255)                          cTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGaa                                                                  >  2:238857/1‑100 (MQ=255)                          cTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGaa                                                                  >  1:1496096/1‑100 (MQ=255)                             gTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACg                                                               >  2:1100229/1‑100 (MQ=255)                               tCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGcc                                                             >  2:224447/1‑100 (MQ=255)                               tCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGcc                                                             <  1:769687/100‑1 (MQ=255)                                  tGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCa                                                          >  2:177262/1‑100 (MQ=255)                                    ggTTCTAGTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGc                                                        <  1:361559/100‑1 (MQ=255)                                        cTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGa                                                    <  1:1339271/100‑1 (MQ=255)                                         tATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGaa                                                   >  1:717589/1‑100 (MQ=255)                                              cGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAc                                              >  1:1334972/1‑100 (MQ=255)                                                 gggAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGa                                           >  2:650794/1‑100 (MQ=255)                                                    aGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCg                                            <  2:1166385/96‑1 (MQ=255)                                                     ncGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCg                                       >  1:58458/2‑100 (MQ=255)                                                     gCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCg                                       <  1:556474/100‑1 (MQ=255)                                                       gTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGGGGGCGTGGAAACGCCTCAGCCGGACCTGACCGAGGCGGt                                     >  2:1499670/1‑100 (MQ=255)                                                       gTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGt                                     <  1:410246/100‑1 (MQ=255)                                                       gTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGt                                     >  1:748316/1‑100 (MQ=255)                                                         aaaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGtat                                   >  2:617259/1‑100 (MQ=255)                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAAcn                               <  2:58458/100‑2 (MQ=255)                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACt                               <  2:785199/100‑1 (MQ=255)                                                               gTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTa                             >  2:754694/1‑100 (MQ=255)                                                                 cACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAAc                           <  1:65902/100‑1 (MQ=255)                                                                     ccagccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAg                       >  1:568944/1‑100 (MQ=255)                                                                        gccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCg                    >  2:1488492/1‑100 (MQ=255)                                                                         ccagTTTGTGGCATCATCCGAGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGc                   >  2:966473/1‑100 (MQ=255)                                                                           agTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGccc                 >  2:1162645/1‑100 (MQ=255)                                                                                gtgGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCg            <  1:195336/100‑1 (MQ=255)                                                                                  ggCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc          >  1:1420297/1‑100 (MQ=255)                                                                                  ggCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc          >  1:434600/1‑100 (MQ=255)                                                                                     atcatcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAgg       <  1:1411158/100‑1 (MQ=255)                                                                                      tcatcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGt      >  2:784541/1‑100 (MQ=255)                                                                                        atcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc    >  1:1278588/1‑100 (MQ=255)                                                                                          ccGGGGTTGGTGTTGATTCATGTGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAg  >  2:1368332/1‑100 (MQ=255)                                                                                          ccGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAg  <  1:1162650/100‑1 (MQ=255)                                                                                               |                                                                                               ATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAG  >  NC_000913/1946696‑1946884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑