Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,971,761 T→C T311A (ACC→GCC)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,7610TC98.1% 179.6 / ‑4.2 54T311A (ACC→GCC) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (25/27);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

CACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGG  >  NC_000913/1971666‑1971856
                                                                                               |                                                                                               
caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                             >  1:776042/1‑100 (MQ=255)
 accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgct                                                                                            >  2:1071207/1‑100 (MQ=255)
  ccacTTTTCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctg                                                                                           >  1:827529/1‑100 (MQ=255)
  ccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctg                                                                                           <  2:771457/100‑1 (MQ=255)
   cacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctgc                                                                                          >  1:709375/1‑100 (MQ=255)
         gccgccGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATg                                                                                    >  1:1087720/1‑100 (MQ=255)
         gccgccGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATg                                                                                    >  1:110572/1‑100 (MQ=255)
          ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                   <  2:897130/100‑1 (MQ=255)
            gccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTg                                                                                 <  1:347567/100‑1 (MQ=255)
             ccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTgg                                                                                <  1:1010564/100‑1 (MQ=255)
              cgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggc                                                                               <  2:146205/100‑1 (MQ=255)
              cgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggc                                                                               <  1:581366/100‑1 (MQ=255)
                 gCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcggc                                                                            <  2:134540/100‑1 (MQ=255)
                         gtgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttctt                                                                    <  1:992889/100‑1 (MQ=255)
                         gtgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttctt                                                                    >  2:906428/1‑100 (MQ=255)
                          tgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttcttc                                                                   >  1:1075230/1‑100 (MQ=255)
                             cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg                                                                <  1:351196/100‑1 (MQ=255)
                             cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg                                                                <  1:771502/100‑1 (MQ=255)
                               gAGGCACTTTGCGCCAGTTGCGTCGCCTGGCGGGCGCTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgcg                                                              >  2:914887/1‑100 (MQ=37)
                               gAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                               >  2:133671/1‑99 (MQ=255)
                                 ggCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCATTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcg                                                            >  1:352701/1‑100 (MQ=255)
                                  gCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg                                                           <  1:1420343/100‑1 (MQ=255)
                                  gCACCTTGCGCCAGCTGCCAGGCCTGGCGGGCGTTATCGGCGTGCTGCGTCACTGTCGCGGCGCGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg                                                           <  2:1102878/100‑1 (MQ=37)
                                   cACTTTGCGCCAGTTGCGCGGCACGGCGGGAGTTCTCGGCGTTTTGCTTCACTGTCGGGGGGAGCGGATCCATGCTGGGGGCAGTCTCTTGCAtcg                                                              >  2:1253686/1‑96 (MQ=255)
                                    aCTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGAt                                                         >  1:1566091/1‑100 (MQ=255)
                                     cTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATg                                                        <  2:452783/100‑1 (MQ=255)
                                      tttGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGc                                                       <  1:1151736/100‑1 (MQ=255)
                                         gcgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                    <  1:1340860/100‑1 (MQ=255)
                                         gcgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                    <  2:772854/100‑1 (MQ=255)
                                          cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   >  2:1520692/1‑100 (MQ=255)
                                          cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                    >  2:1162418/1‑99 (MQ=255)
                                            ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                 <  2:319955/100‑1 (MQ=255)
                                               gTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTc                                              <  1:599684/100‑1 (MQ=255)
                                                  gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                           >  1:1393444/1‑100 (MQ=255)
                                                  gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGAAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                           >  1:1229884/1‑100 (MQ=255)
                                                    gAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTAc                                         >  2:1553717/1‑100 (MQ=255)
                                                        ccTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGgga                                     >  1:1124808/1‑100 (MQ=255)
                                                         cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag                                    >  2:948049/1‑100 (MQ=255)
                                                            gcgggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGaa                                 <  2:1499829/100‑1 (MQ=255)
                                                              gggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg                               <  1:1533723/100‑1 (MQ=255)
                                                                 cgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                            >  1:1019082/1‑100 (MQ=255)
                                                                   ttATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGTGAGGAAAGATCgg                          <  1:1071213/100‑1 (MQ=255)
                                                                   ttATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgg                          <  2:1240743/100‑1 (MQ=255)
                                                                       cGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt                      >  2:279645/1‑100 (MQ=255)
                                                                       cGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAAGAAAGATCGGTGtt                      >  2:1455316/1‑100 (MQ=255)
                                                                        ggCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTg                     <  2:1393436/100‑1 (MQ=255)
                                                                         gCGTTTTGCTTCACTATCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGc                    <  1:1553573/100‑1 (MQ=255)
                                                                          cGTTGTGCTGAACCGTCTCGGCGAGTTGCTCCAATCTGGCTGCAGATTCTTCCAGCGCGGATGCCGCCTGTTCAGTACGGGAGGAAAGATCGgtggtag                    <  2:1422602/99‑5 (MQ=255)
                                                                            ttttGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGCTGCCTGCTGTTCAGTCCGGGCGGAACGATCGCTGttcacc                  >  2:1571114/1‑95 (MQ=37)
                                                                               tGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg              <  1:1250133/100‑1 (MQ=255)
                                                                                   gtgaaattgcggcgAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGCTGCCCGCCGCAAt          <  1:816442/92‑1 (MQ=255)
                                                                                    cACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAtt         <  1:1058329/100‑1 (MQ=255)
                                                                                      cTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTc       >  2:279563/1‑100 (MQ=255)
                                                                                       tGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCa      <  2:1087715/100‑1 (MQ=255)
                                                                                        gTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCAc     >  1:25442/1‑100 (MQ=255)
                                                                                           gcggcgAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg  <  2:929270/100‑1 (MQ=255)
                                                                                               |                                                                                               
CACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGG  >  NC_000913/1971666‑1971856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑