| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,983,761 | C→T | E437K (GAA→AAA) | araG ← | L‑arabinose ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,983,761 | 0 | C | T | 97.9% | 161.1 / ‑4.3 | 48 | E437K (GAA→AAA) | araG | L‑arabinose ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (25/22); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGC > NC_000913/1983664‑1983855 | aTCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGt < 2:304472/100‑1 (MQ=255) tCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTtgtg < 1:1368511/100‑1 (MQ=255) ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGc > 2:796557/1‑100 (MQ=255) ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGc < 1:129488/100‑1 (MQ=255) caggTCGGAAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATACTTTGTGc < 2:1237765/98‑1 (MQ=255) ggTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCtt < 1:856465/100‑1 (MQ=255) ggTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCtt > 2:813667/1‑100 (MQ=255) gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc > 1:273561/1‑100 (MQ=255) cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc > 1:106528/1‑100 (MQ=255) cGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc > 1:100967/1‑100 (MQ=255) aCTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGc < 2:614655/100‑1 (MQ=255) aCTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGc > 1:1418353/1‑100 (MQ=255) ttCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc < 2:1330140/99‑1 (MQ=255) tCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgc > 2:1094355/1‑100 (MQ=255) ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgcg > 2:1211024/1‑100 (MQ=255) aaGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGAGTAg > 2:1435107/1‑100 (MQ=255) aGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAgg < 1:622238/100‑1 (MQ=255) cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc > 2:820220/1‑100 (MQ=255) cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc > 1:760105/1‑100 (MQ=255) cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCAAACATCAATGCCGCGCGTAGGTTc > 2:1574024/1‑100 (MQ=255) aGGCAACCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAACTTACGTTCTATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAg < 2:1318249/100‑1 (MQ=255) aGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAg < 2:465585/100‑1 (MQ=255) cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa > 2:1361231/1‑100 (MQ=255) cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa > 2:362191/1‑100 (MQ=255) cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCa > 2:903350/1‑99 (MQ=255) aaCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaaaa < 2:1122846/100‑1 (MQ=255) cAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATg > 2:750349/1‑100 (MQ=255) cGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTc < 2:487859/100‑1 (MQ=255) gCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCa < 1:937767/100‑1 (MQ=255) gCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATctct > 2:1462727/1‑100 (MQ=255) cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGAAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc > 1:1632/1‑100 (MQ=255) tGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCg > 2:749422/1‑100 (MQ=255) gcgcCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc < 2:562020/98‑1 (MQ=255) gccgccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATa < 1:749424/100‑1 (MQ=255) gcgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCg > 2:1490932/1‑100 (MQ=255) cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCgg < 1:376448/100‑1 (MQ=255) tAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCa > 1:1208911/1‑100 (MQ=255) aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg < 2:100967/100‑1 (MQ=255) aaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGa < 1:1309468/100‑1 (MQ=255) ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAAt < 1:1134245/100‑1 (MQ=255) ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAAt < 1:424134/100‑1 (MQ=255) tACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATg < 2:374976/100‑1 (MQ=255) aCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATgg < 2:498043/100‑1 (MQ=255) aCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATgg < 1:1447616/100‑1 (MQ=255) cGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGc > 2:1087026/1‑100 (MQ=255) ttATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtt > 1:721592/1‑100 (MQ=255) tatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttt < 2:982758/100‑1 (MQ=255) tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc > 1:171540/1‑100 (MQ=255) tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc > 1:552082/1‑100 (MQ=255) | ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGC > NC_000913/1983664‑1983855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |