Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,071,321	A→G	intergenic (+110/‑218)	yeeP → / → flu	pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,071,321	0	A	G	100.0%	277.2 / NA	77	intergenic (+110/‑218)	yeeP/flu	pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/44);  total (33/44)

TGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGA  >  NC_000913/2071224‑2071415                                                                                                  |                                                                                               tGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAAcgatcgat                                                                                              <  2:1367385/100‑1 (MQ=255)  gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                             >  2:1320372/1‑100 (MQ=255)  gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTCTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                             >  2:1539544/1‑100 (MQ=255)    tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCCATAGCTCAATAATAGAATAAAACGATCGATATc                                                                                           >  1:937103/1‑100 (MQ=255)     gCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCt                                                                                          <  1:125657/100‑1 (MQ=255)        ctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtt                                                                                       >  1:1543874/1‑100 (MQ=255)        ctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtt                                                                                       <  1:313748/100‑1 (MQ=255)         tccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAttt                                                                                      <  2:360026/100‑1 (MQ=255)          ccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAAGAGAATAAAACGCTCGATGTCTAtttt                                                                                     >  2:1509991/1‑100 (MQ=255)           cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTa                                                                                    <  1:1345679/100‑1 (MQ=255)             tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatc                                                                                  <  1:395215/100‑1 (MQ=255)             tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatc                                                                                  <  2:610243/100‑1 (MQ=255)             tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatc                                                                                  <  1:1433829/100‑1 (MQ=255)                 tctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcgatc                                                                              <  2:1345595/100‑1 (MQ=255)                   tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                            >  1:1755/1‑100 (MQ=255)                    gAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGtt                                                                           >  2:422543/1‑100 (MQ=255)                    gAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGtt                                                                           >  1:136729/1‑100 (MQ=255)                     aGTCTGCTCACCAAAGCACTGTTTTTGTTACTGTGTCTCTTGTCCGTGCAATAGCTCAATAAGAGAATAAAACGATCGATATCTATTTTATCGATCGttt                                                                          <  2:676891/100‑1 (MQ=255)                       tCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                            <  2:1121174/96‑1 (MQ=255)                          gCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatc                                                                     >  1:42765/1‑100 (MQ=255)                           cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcg                                                                    >  1:1475382/1‑100 (MQ=255)                             cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgat                                                                  >  2:1230650/1‑100 (MQ=255)                              acaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatc                                                                 >  1:1562168/1‑100 (MQ=255)                                aaaaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATAtcgatcga                                                               >  2:505712/1‑100 (MQ=255)                                 aaaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATcgatcgat                                                              <  2:790144/100‑1 (MQ=255)                                  aaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATa                                                             <  1:870485/100‑1 (MQ=255)                                      aCTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGc                                                          <  2:1755/99‑1 (MQ=255)                                       cTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCta                                                        >  2:1566200/1‑100 (MQ=255)                                         gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaat                                                      <  1:240117/100‑1 (MQ=255)                                          ttttCGTTATTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATAACTATTTTATCGATCGTTTATATCGATCGATAAGCtaata                                                     <  2:1464093/100‑1 (MQ=255)                                          ttttCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaata                                                     <  2:1028347/100‑1 (MQ=255)                                           tttCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataa                                                    <  1:534428/100‑1 (MQ=255)                                             tCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                  <  1:482779/100‑1 (MQ=255)                                               gatcttGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAc                                                <  1:292405/95‑1 (MQ=255)                                                ttACTGTCTCTCTTGTCCGTGCAATATCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAc                                                <  2:136729/99‑1 (MQ=255)                                                    tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAcagatc                                           >  2:292405/1‑95 (MQ=255)                                                    tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                           <  2:1542966/100‑1 (MQ=255)                                                      tctctcTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTc                                         <  2:172954/100‑1 (MQ=255)                                                      tctctcTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTc                                         <  1:1320379/100‑1 (MQ=255)                                                          tctTGTCCGTGCAATAGCTCAATAATATAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAg                                       <  2:1438227/98‑1 (MQ=255)                                                          tctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTa                                     <  1:691422/100‑1 (MQ=255)                                                           ctTGTCCGTGCAATAGCTCAATATTAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTaa                                    >  2:192029/1‑100 (MQ=255)                                                            ttGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                   <  1:1575756/100‑1 (MQ=255)                                                             tGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACa                                  >  2:908640/1‑100 (MQ=255)                                                             tGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACa                                  >  2:1366984/1‑100 (MQ=255)                                                              gTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACAt                                 >  1:1173016/1‑100 (MQ=255)                                                              gTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACAt                                 >  1:692136/1‑100 (MQ=255)                                                               tCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATg                                <  2:288341/100‑1 (MQ=255)                                                               tCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATg                                <  1:158931/100‑1 (MQ=255)                                                                tcGTGCAATAGCGTACTAATAGAATAAAACGATCGATAACTATTTTACCGATAGTTTATATAGATCTATAAGCAAATAATAACCTTTATCAGt                                      >  2:468176/2‑93 (MQ=255)                                                                 cGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGca                              >  2:1409235/1‑100 (MQ=255)                                                                  gTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcac                             <  2:1543864/100‑1 (MQ=255)                                                                  gTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcac                             >  1:382583/1‑100 (MQ=255)                                                                  gTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcac                             <  1:305913/100‑1 (MQ=255)                                                                   tGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcaca                            <  1:1164977/100‑1 (MQ=255)                                                                    gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                           >  2:1365987/1‑100 (MQ=255)                                                                       aTAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATa                        >  1:1099306/1‑100 (MQ=255)                                                                          gCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGt                     >  2:618184/1‑100 (MQ=255)                                                                             cAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACa                  >  2:1554740/1‑100 (MQ=255)                                                                              aataatAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAg                 <  2:42765/100‑1 (MQ=255)                                                                              aataatAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAg                 <  2:1303451/100‑1 (MQ=255)                                                                               ataataGAATAAAGCGGTCGATATCTATTTTATCGATCGTTTATATCGATCGATGAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGa                <  2:1534696/100‑1 (MQ=255)                                                                                taataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaa               <  2:937098/100‑1 (MQ=255)                                                                                 aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa              <  1:994297/100‑1 (MQ=255)                                                                                  ataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAg             >  1:692147/1‑100 (MQ=255)                                                                                  ataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAg             <  2:1073001/100‑1 (MQ=255)                                                                                   taGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGa            >  2:1225270/1‑100 (MQ=255)                                                                                   taGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGa            <  2:1498947/100‑1 (MQ=255)                                                                                     gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGAc           >  1:770829/1‑99 (MQ=255)                                                                                      aaTAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCAGAGATACGTACAGAAAGACAt         <  2:969605/100‑1 (MQ=255)                                                                                       aTAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACAtt        >  1:670275/1‑100 (MQ=255)                                                                                        tAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTc       <  2:481096/100‑1 (MQ=255)                                                                                          aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg     >  2:139609/1‑100 (MQ=255)                                                                                          aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg     <  2:821883/100‑1 (MQ=255)                                                                                          aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg     >  1:389631/1‑100 (MQ=255)                                                                                           aaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAgg    <  1:1086544/100‑1 (MQ=255)                                                                                           aaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAgg    <  1:574622/100‑1 (MQ=255)                                                                                           aaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAgg    <  1:289280/100‑1 (MQ=255)                                                                                             cgatcgatATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGa  >  1:668026/1‑100 (MQ=255)                                                                                                  |                                                                                               TGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGA  >  NC_000913/2071224‑2071415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑