Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,191,432 | C→T | G72S (GGC→AGC) | yehC ← | putative periplasmic pilin chaperone |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,191,432 | 0 | C | T | 98.3% | 201.9 / ‑4.2 | 60 | G72S (GGC→AGC) | yehC | putative periplasmic pilin chaperone |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (32/27); total (32/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 |
ATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCG > NC_000913/2191335‑2191525 | aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtat < 1:579576/100‑1 (MQ=255) tttttACTTGCTGCCCGGAATTTGCCACTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc < 2:611926/100‑1 (MQ=255) ttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTatcatc < 1:182459/100‑1 (MQ=255) ttACTTGCTGCCCGGAATTTGCCCCTATTCTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAACTTTTTCTGGTGGTAATGACGTATCGCTatcatc < 2:947080/100‑1 (MQ=255) tACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCAtcatca < 1:261156/100‑1 (MQ=255) ttGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATc < 2:573462/100‑1 (MQ=255) tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc > 1:471461/1‑100 (MQ=255) ctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCAc < 1:1360426/100‑1 (MQ=255) gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc > 1:1095786/1‑100 (MQ=255) gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc > 1:81097/1‑100 (MQ=255) ccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCt > 2:389911/1‑100 (MQ=255) tttGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgcagca < 2:574131/100‑1 (MQ=255) ttGCCCCTATTTTTGCTACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGcagcag > 2:1113826/1‑100 (MQ=255) gCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCg > 1:1150557/1‑100 (MQ=255) ccccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGTCGTATCGCTATCATCAATCCACGCCTGCAGCAGCga < 1:154159/100‑1 (MQ=255) ccccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCga > 1:24473/1‑100 (MQ=255) cccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaa > 1:1213542/1‑100 (MQ=255) cccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaa > 1:1404724/1‑100 (MQ=255) ccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaag > 1:222497/1‑100 (MQ=255) ccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGCATCGCTATCATCAATCCACGCCTGCAGCAGCgaag > 1:1504986/1‑100 (MQ=255) cTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaaga < 2:1018352/100‑1 (MQ=255) aattttGCCACTGATGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAAc > 1:1072202/3‑100 (MQ=255) gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc < 1:154573/100‑1 (MQ=255) gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc > 1:1019780/1‑100 (MQ=255) ccatgggtggCGTTAACATGAAAGGGACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc < 1:1477736/95‑1 (MQ=255) tggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTgg < 2:964620/100‑1 (MQ=255) tggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTgg < 1:1297794/100‑1 (MQ=255) ggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGt > 1:1202507/1‑100 (MQ=255) ggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGt > 1:1202529/1‑100 (MQ=255) ggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGt > 2:156428/1‑100 (MQ=255) gtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGtt < 2:1202524/100‑1 (MQ=255) gtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGtt < 2:1202502/100‑1 (MQ=255) gtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGtt > 1:673317/1‑100 (MQ=255) tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc < 2:1019774/100‑1 (MQ=255) tagCGGTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc < 2:724702/98‑1 (MQ=255) ggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGTTTTCCCTGGTtca < 1:293757/100‑1 (MQ=255) cGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCCATCCTCGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatc < 2:1127145/100‑1 (MQ=255) cGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatc < 1:389911/100‑1 (MQ=255) tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGGCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc > 2:1109146/1‑100 (MQ=255) ngAAAGGAACCTGAATTTTTTTTTTTTTTATTGACGTATCGCTATCATCAATCCACGCCTGTAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc > 2:1276056/2‑100 (MQ=255) gacacgAACCTGAAGCTTGTCTGGTGGTAAAGAGGTAGCGCTACCATCCATCCTCGCCTTCAGCAGCGAAGAACGGCTGCCCTGGTGCATCAGCTGc < 2:1507899/92‑1 (MQ=255) gAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATAAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAcc > 2:850992/1‑100 (MQ=255) ggAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCa > 1:664039/1‑100 (MQ=255) ccTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtc > 1:795700/1‑100 (MQ=255) cTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCGATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtct > 1:1084889/1‑100 (MQ=255) tGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtctt < 2:12081/100‑1 (MQ=255) aaTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTa < 2:81097/100‑1 (MQ=255) aaTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTa < 2:721553/100‑1 (MQ=255) aTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:1274619/1‑100 (MQ=255) aTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:1274220/1‑100 (MQ=255) ttttttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAtt < 1:163125/100‑1 (MQ=255) tttttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttt < 1:156428/100‑1 (MQ=255) ttttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAtttt > 2:381032/1‑100 (MQ=255) tttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTc > 1:1190050/1‑100 (MQ=255) ttCTGGTGGTAATTACGTATCGCTAGCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTGTca < 2:760962/100‑2 (MQ=255) tggtggTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCtcc > 2:1262936/1‑97 (MQ=255) ggtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg > 1:64116/1‑100 (MQ=255) gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTACCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg > 1:715900/1‑100 (MQ=255) ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCGTCACTTCTTTATTTTCTGCCGGGt > 1:1098982/1‑100 (MQ=255) ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGt < 2:1335209/100‑1 (MQ=255) aTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaat > 1:594794/1‑100 (MQ=255) tATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcg < 1:714391/100‑1 (MQ=255) | ATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCG > NC_000913/2191335‑2191525 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |