| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,197,365 | G→A | pseudogene (892/2847 nt) | yehH → | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,197,365 | 0 | G | A | 94.4% | 157.8 / ‑1.2 | 54 | pseudogene (892/2847 nt) | yehH | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (26/25); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCG > NC_000913/2197268‑2197456 | cTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTAcc < 1:1276280/100‑1 (MQ=255)cTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTAcc > 2:1187003/1‑100 (MQ=255) aCTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGc > 2:1461204/1‑100 (MQ=255) cTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCa > 2:501672/1‑100 (MQ=255) cTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCa > 2:1200527/1‑100 (MQ=255) tGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAt > 2:1322297/1‑100 (MQ=255) ggAGTAATATTCCAGATTTGAATGACGCCTACGTAACCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAtt < 1:308580/100‑1 (MQ=255) ggAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAtt > 1:63438/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAtt > 2:934545/1‑100 (MQ=255) tCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTc > 2:523689/1‑100 (MQ=255) ccAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTc > 1:62338/1‑99 (MQ=255) ccgATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCa > 1:1072422/3‑100 (MQ=255) cAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCa < 2:1025616/100‑1 (MQ=255) aGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAg < 2:658117/100‑1 (MQ=255) gAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggc < 1:1095503/100‑1 (MQ=255) aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcg > 1:1407946/1‑100 (MQ=255) aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcg < 1:469820/100‑1 (MQ=255) aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcg > 2:172720/1‑100 (MQ=255) aTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAATAACGTATTACCGCAATTCCACATATTCCAGAATggcg > 2:858789/1‑99 (MQ=255) aTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcgg < 1:568172/100‑1 (MQ=255) aTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcgg < 1:430219/100‑1 (MQ=255) gCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAAcc < 2:62338/100‑1 (MQ=255) ccgatcctAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAAcc < 1:25524/93‑1 (MQ=255) ccTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCa < 1:1247094/100‑1 (MQ=255) tACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCAtt < 1:1199510/100‑1 (MQ=255) tACGAAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAAGTCCACATATTCCAGAATGGCGGCAACCAtn < 2:1441562/100‑2 (MQ=255) cGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTaa < 1:996105/100‑1 (MQ=255) tAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCagatcgg > 2:25524/1‑94 (MQ=255) tAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAAtt > 1:983453/1‑100 (MQ=255) aCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCt < 2:1402011/100‑1 (MQ=255) aCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCt < 1:173841/100‑1 (MQ=255) ttATCACTGACGTAATAGGCTCTCTGTCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATGTTCCAGAATGGCGGCAACCATTAATTGCttn < 2:333946/98‑2 (MQ=255) ttATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACTTATTCCAGAATGGCGGCAACCATTAATTGCTTTGt < 2:995295/100‑1 (MQ=255) aTCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtta < 2:669603/100‑1 (MQ=255) tCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttat > 2:1087292/1‑100 (MQ=255) gACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTAccc < 1:897383/100‑1 (MQ=255) gagTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGTCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAc > 2:1068438/1‑100 (MQ=255) gTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCa > 1:1267022/1‑100 (MQ=255) ctctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGa > 1:1336765/1‑100 (MQ=255) ctctctGGAAAAAAAAAACAAAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGa < 2:1537452/100‑1 (MQ=255) tctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGaa < 2:531350/100‑1 (MQ=255) ctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAAtt < 1:867987/100‑1 (MQ=255) ggCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGc < 2:1336758/100‑1 (MQ=255) ggCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGc > 1:1194764/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 2:1578019/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 2:142441/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 2:1004171/1‑100 (MQ=255) gCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTACTTCGTTATTACCCGACAAAACAGAAATTGCACAGGaactcg > 2:697278/1‑96 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGc > 2:189422/1‑100 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGc < 1:845380/100‑1 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc > 2:1183505/1‑100 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc < 2:584578/100‑1 (MQ=255) aaaaCTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAg < 1:1087297/100‑1 (MQ=255) aaCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCg > 2:131363/1‑100 (MQ=255) | CTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCG > NC_000913/2197268‑2197456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |