Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,235,656	T→C	I131T (ATC→ACC)	preA →	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,235,656	0	T	C	100.0%	215.8 / NA	59	I131T (ATC→ACC)	preA	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

GGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT  >  NC_000913/2235564‑2235752                                                                                             |                                                                                                 ggACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAgtgt                                                                                           <  2:254699/100‑1 (MQ=255)    cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGCTATGACCGCgtgtc                                                                                          >  1:1477057/1‑97 (MQ=255)     aaaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACt                                                                                       <  2:740842/100‑1 (MQ=255)      aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt                                                                                      <  1:1006023/100‑1 (MQ=255)       aGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtc                                                                                     >  1:669296/1‑100 (MQ=255)       aGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGAGCGGATATGACCGAGTGTAACTtc                                                                                     <  2:131546/100‑1 (MQ=255)         tATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtctc                                                                                   >  1:325522/1‑100 (MQ=255)          aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                  <  1:668659/100‑1 (MQ=255)          aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                  >  2:1520366/1‑100 (MQ=255)          aTTGATCGCTTCGAGCATGGGAGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCTGATATGACCGAGTGTAACTTCTcc                                                                                  <  2:1106089/100‑1 (MQ=255)             gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGt                                                                               >  1:1468486/1‑100 (MQ=255)              aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTc                                                                              <  2:1089351/100‑1 (MQ=255)                cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                            >  1:1307616/1‑100 (MQ=255)                cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                            >  1:1572653/1‑100 (MQ=255)                cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                            <  1:1581640/100‑1 (MQ=255)                cGCCTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                            <  1:1386404/100‑1 (MQ=255)                      gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                      >  2:693339/1‑100 (MQ=255)                      gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                      <  1:682793/100‑1 (MQ=255)                       aTCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGa                                                                     >  1:557664/1‑100 (MQ=255)                                gAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                            <  2:1344550/100‑1 (MQ=255)                                  aaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                                          <  2:653513/100‑1 (MQ=255)                                   aaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGa                                                         >  1:686678/1‑100 (MQ=255)                                      gAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                                                      <  1:657857/100‑1 (MQ=255)                                      gAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                                                      <  1:658110/100‑1 (MQ=255)                                       agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATggg                                                     <  1:1141427/100‑1 (MQ=255)                                       agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATggg                                                     >  1:756881/1‑100 (MQ=255)                                        gcagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGt                                                    <  1:139524/100‑1 (MQ=255)                                        gcagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGt                                                    >  2:782088/1‑100 (MQ=255)                                           gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                 >  2:534597/1‑100 (MQ=255)                                           gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                 >  2:664664/1‑100 (MQ=255)                                           gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                 >  2:72301/1‑100 (MQ=255)                                            taaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                <  1:1035362/99‑1 (MQ=255)                                            caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                >  2:1006533/1‑100 (MQ=255)                                             aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                               <  1:125062/100‑1 (MQ=255)                                             aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGATGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGCTGGGTAGCGa                                               >  2:1035356/1‑100 (MQ=255)                                                 ggaggaGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTc                                           <  2:972753/100‑1 (MQ=255)                                                    ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                        <  2:1462921/100‑1 (MQ=255)                                                     gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc                                       <  1:219412/100‑1 (MQ=255)                                                      agCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa                                      >  2:1183797/1‑100 (MQ=255)                                                      agCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa                                      >  1:1398832/1‑100 (MQ=255)                                                         tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                   >  1:1372013/1‑100 (MQ=255)                                                          ggCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                  >  1:135899/1‑100 (MQ=255)                                                               gcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGCAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                              >  2:846954/1‑99 (MQ=255)                                                                 cTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGc                           <  2:756879/100‑1 (MQ=255)                                                                  tGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                          >  2:69997/1‑100 (MQ=255)                                                                  tGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                          <  2:106991/100‑1 (MQ=255)                                                                    gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                        <  1:762949/100‑1 (MQ=255)                                                                     tGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                       <  2:1096627/100‑1 (MQ=255)                                                                      gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCTGAGCTGGTa                      >  2:343705/1‑100 (MQ=255)                                                                        aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                    >  1:408871/1‑100 (MQ=255)                                                                        aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                    >  2:1492306/1‑100 (MQ=255)                                                                            agCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaaa                <  1:664666/100‑1 (MQ=255)                                                                              cTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata              <  1:270430/100‑1 (MQ=255)                                                                              cTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata              <  1:1403061/100‑1 (MQ=255)                                                                              cTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata              <  1:693341/100‑1 (MQ=255)                                                                                 gcgcGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTg           <  2:970870/100‑1 (MQ=255)                                                                                   gcgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTc         <  1:1183802/100‑1 (MQ=255)                                                                                      gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCggg      >  1:1144067/1‑100 (MQ=255)                                                                                          tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt  >  1:779347/1‑100 (MQ=255)                                                                                             |                                                                                                 GGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT  >  NC_000913/2235564‑2235752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑