Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,246,800 A→G intergenic (‑31/+263) cirA ← / ← lysP colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,246,8000AG98.2% 182.0 / ‑4.1 54intergenic (‑31/+263)cirA/lysPcolicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (29/24);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

CAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCC  >  NC_000913/2246703‑2246895
                                                                                                 |                                                                                               
caGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaa                                                                                               >  2:1338221/1‑100 (MQ=255)
caGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaa                                                                                               >  1:613561/1‑100 (MQ=255)
 aGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaaa                                                                                              <  2:691409/100‑1 (MQ=255)
  ggCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaaat                                                                                             >  2:263843/1‑100 (MQ=255)
   gCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAaaatgaaatg                                                                                            <  2:641367/100‑1 (MQ=255)
   gCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAaaatgaaatg                                                                                            <  2:887599/100‑1 (MQ=255)
    ccATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATgg                                                                                           >  2:1186872/1‑100 (MQ=255)
     cATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATggg                                                                                          >  2:410445/1‑100 (MQ=255)
         cacaAGAAATAGAGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATggg                                                                                          <  2:1053716/96‑1 (MQ=255)
              gAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTc                                                                                 >  1:813963/1‑100 (MQ=255)
              gAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTc                                                                                 >  2:931447/1‑100 (MQ=255)
                 aTAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTcaca                                                                              >  1:676818/1‑100 (MQ=255)
                   aGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCTACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtc                                                                            >  2:844138/1‑100 (MQ=255)
                   aGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtc                                                                            <  2:639737/100‑1 (MQ=255)
                    gCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtct                                                                           <  2:577583/100‑1 (MQ=255)
                      gatctaaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttc                                                                         <  1:332421/95‑1 (MQ=255)
                       gACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttct                                                                        <  2:936466/100‑1 (MQ=255)
                         cAAACACAGCCCGACCCGTACGAAAGGGTTCAACATAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCTCTCACATCttcttc                                                                      >  2:1325672/1‑100 (MQ=255)
                           aaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttcagatc                                                                    >  2:332421/1‑95 (MQ=255)
                           aaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCAc                                                                    >  2:315843/1‑100 (MQ=255)
                            acacAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACg                                                                   >  1:386647/1‑100 (MQ=255)
                            acacAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACg                                                                   <  1:616881/100‑1 (MQ=255)
                              acaGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTc                                                                 >  2:360520/1‑100 (MQ=255)
                                   ccGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCtt                                                            >  1:1300955/1‑100 (MQ=255)
                                    cGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTg                                                           <  1:725197/100‑1 (MQ=255)
                                    cGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTg                                                           <  1:725185/100‑1 (MQ=255)
                                         cGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAc                                                      <  1:502232/100‑1 (MQ=255)
                                         cGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAc                                                      <  1:254594/100‑1 (MQ=255)
                                            aCGAAAGGATTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgc                                                   <  2:585857/100‑1 (MQ=255)
                                             cGAAAGGGTTCAACCTAAACATTCCATATCTCCGTGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgc                                                   >  2:946243/1‑99 (MQ=255)
                                             cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGGTCACATCTTCTTCACGTCCCCTTGCGTACGgca                                                  >  2:684854/1‑100 (MQ=255)
                                             cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgca                                                  <  2:1535806/100‑1 (MQ=255)
                                             cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgca                                                  >  1:1085251/1‑100 (MQ=255)
                                                aaGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCACTTGCGTACGgcagc                                                >  2:738708/1‑99 (MQ=255)
                                                   ggTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcg                                            >  2:1298598/1‑100 (MQ=255)
                                                      tCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg                                         >  2:58358/1‑100 (MQ=255)
                                                      tCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg                                         <  1:771115/100‑1 (MQ=255)
                                                         aCCTAAACATTCCATGCCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg                                      <  1:204439/100‑1 (MQ=255)
                                                         aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg                                      <  2:69685/100‑1 (MQ=255)
                                                         aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg                                      <  2:909525/100‑1 (MQ=255)
                                                         aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg                                      >  1:1131320/1‑100 (MQ=255)
                                                         aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg                                      >  1:546685/1‑100 (MQ=255)
                                                          ccTAAACATTCTATATCTCCATGAGGTAACTACGAAAATGACATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa                                     <  2:334535/100‑1 (MQ=255)
                                                          ccTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa                                     >  1:512453/1‑100 (MQ=255)
                                                          ccTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa                                     >  1:806863/1‑100 (MQ=255)
                                                           cTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGAc                                    >  1:98004/1‑100 (MQ=255)
                                                                   tCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt                            >  2:1423762/1‑100 (MQ=255)
                                                                   tCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt                            <  1:263843/100‑1 (MQ=255)
                                                                   tCCATATCTCCATGAGGTAACTACGAAAACGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt                            <  2:948371/100‑1 (MQ=255)
                                                                    ccATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAtttttt                           >  1:739252/1‑100 (MQ=255)
                                                                     cATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTa                          <  2:1458733/100‑1 (MQ=255)
                                                                     cATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTa                          <  2:930836/100‑1 (MQ=255)
                                                                       tatCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATg                        >  2:990988/1‑100 (MQ=255)
                                                                        atCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGc                       <  1:844143/100‑1 (MQ=255)
                                                                           tcCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAgg                    <  1:1521711/100‑1 (MQ=255)
                                                                           tcCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAgg                    <  1:1423770/100‑1 (MQ=255)
                                                                                gAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGatc               >  1:847085/1‑100 (MQ=255)
                                                                                      aCTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCa         <  2:546684/100‑1 (MQ=255)
                                                                                        tACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAAGGCTGCATCGCAGTGGACTTATTTTTTATGCAGGTGATCATcc           >  2:225840/1‑96 (MQ=255)
                                                                                          cGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGaaa     >  1:821754/1‑100 (MQ=255)
                                                                                             aaatgaaatgGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGcc  <  1:1186877/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCC  >  NC_000913/2246703‑2246895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: