Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,246,800 | A→G | intergenic (‑31/+263) | cirA ← / ← lysP | colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,246,800 | 0 | A | G | 98.2% | 182.0 / ‑4.1 | 54 | intergenic (‑31/+263) | cirA/lysP | colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (29/24); total (29/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 |
CAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCC > NC_000913/2246703‑2246895 | caGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaa > 2:1338221/1‑100 (MQ=255) caGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaa > 1:613561/1‑100 (MQ=255) aGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaaa < 2:691409/100‑1 (MQ=255) ggCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaatgaaat > 2:263843/1‑100 (MQ=255) gCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAaaatgaaatg < 2:641367/100‑1 (MQ=255) gCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAaaatgaaatg < 2:887599/100‑1 (MQ=255) ccATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATgg > 2:1186872/1‑100 (MQ=255) cATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATggg > 2:410445/1‑100 (MQ=255) cacaAGAAATAGAGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATggg < 2:1053716/96‑1 (MQ=255) gAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTc > 1:813963/1‑100 (MQ=255) gAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTc > 2:931447/1‑100 (MQ=255) aTAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTcaca > 1:676818/1‑100 (MQ=255) aGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCTACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtc > 2:844138/1‑100 (MQ=255) aGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtc < 2:639737/100‑1 (MQ=255) gCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtct < 2:577583/100‑1 (MQ=255) gatctaaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttc < 1:332421/95‑1 (MQ=255) gACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttct < 2:936466/100‑1 (MQ=255) cAAACACAGCCCGACCCGTACGAAAGGGTTCAACATAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCTCTCACATCttcttc > 2:1325672/1‑100 (MQ=255) aaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACAtcttcagatc > 2:332421/1‑95 (MQ=255) aaCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCAc > 2:315843/1‑100 (MQ=255) acacAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACg > 1:386647/1‑100 (MQ=255) acacAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACg < 1:616881/100‑1 (MQ=255) acaGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTc > 2:360520/1‑100 (MQ=255) ccGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCtt > 1:1300955/1‑100 (MQ=255) cGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTg < 1:725197/100‑1 (MQ=255) cGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTg < 1:725185/100‑1 (MQ=255) cGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAc < 1:502232/100‑1 (MQ=255) cGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAc < 1:254594/100‑1 (MQ=255) aCGAAAGGATTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgc < 2:585857/100‑1 (MQ=255) cGAAAGGGTTCAACCTAAACATTCCATATCTCCGTGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgc > 2:946243/1‑99 (MQ=255) cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGGTCACATCTTCTTCACGTCCCCTTGCGTACGgca > 2:684854/1‑100 (MQ=255) cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgca < 2:1535806/100‑1 (MQ=255) cGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGgca > 1:1085251/1‑100 (MQ=255) aaGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCACTTGCGTACGgcagc > 2:738708/1‑99 (MQ=255) ggTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcg > 2:1298598/1‑100 (MQ=255) tCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg > 2:58358/1‑100 (MQ=255) tCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg < 1:771115/100‑1 (MQ=255) aCCTAAACATTCCATGCCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg < 1:204439/100‑1 (MQ=255) aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg < 2:69685/100‑1 (MQ=255) aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg < 2:909525/100‑1 (MQ=255) aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg > 1:1131320/1‑100 (MQ=255) aCCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTgg > 1:546685/1‑100 (MQ=255) ccTAAACATTCTATATCTCCATGAGGTAACTACGAAAATGACATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa < 2:334535/100‑1 (MQ=255) ccTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa > 1:512453/1‑100 (MQ=255) ccTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGa > 1:806863/1‑100 (MQ=255) cTAAACATTCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGAc > 1:98004/1‑100 (MQ=255) tCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt > 2:1423762/1‑100 (MQ=255) tCCATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt < 1:263843/100‑1 (MQ=255) tCCATATCTCCATGAGGTAACTACGAAAACGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAttttt < 2:948371/100‑1 (MQ=255) ccATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTAtttttt > 1:739252/1‑100 (MQ=255) cATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTa < 2:1458733/100‑1 (MQ=255) cATATCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTa < 2:930836/100‑1 (MQ=255) tatCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATg > 2:990988/1‑100 (MQ=255) atCTCCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGc < 1:844143/100‑1 (MQ=255) tcCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAgg < 1:1521711/100‑1 (MQ=255) tcCATGAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAgg < 1:1423770/100‑1 (MQ=255) gAGGTAACTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGatc > 1:847085/1‑100 (MQ=255) aCTACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCa < 2:546684/100‑1 (MQ=255) tACGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTAAGGCTGCATCGCAGTGGACTTATTTTTTATGCAGGTGATCATcc > 2:225840/1‑96 (MQ=255) cGAAAATGAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGaaa > 1:821754/1‑100 (MQ=255) aaatgaaatgGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGcc < 1:1186877/100‑1 (MQ=255) | CAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGGTGGACTTATTTTTTATGCAGGTGATCATCCCAGAAAGCC > NC_000913/2246703‑2246895 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |