Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,307,386 A→T D410E (GAT→GAA)  yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,3860AT96.8% 211.4 / ‑1.4 62D410E (GAT→GAA) yojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (24/36);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGC  >  NC_000913/2307289‑2307483
                                                                                                 |                                                                                                 
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc                                                                                                 <  1:444706/100‑1 (MQ=255)
 gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa                                                                                                <  1:526271/100‑1 (MQ=255)
  ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa                                                                                               <  2:123821/100‑1 (MQ=255)
  ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa                                                                                               <  2:1544294/100‑1 (MQ=255)
    gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc                                                                                             <  2:34969/100‑1 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACa                                                                                            >  1:453183/1‑100 (MQ=255)
         ncTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCa                                                                                        >  1:496487/2‑100 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                       <  1:1393225/100‑1 (MQ=255)
           tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                       <  2:490931/99‑1 (MQ=255)
            ccaGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCCGGTTAGCGGCTTTACCCTCCGGCCCCCGCAGTTGTTCAAACAGCCAg                                                                                       >  2:165043/3‑98 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                     >  2:325741/1‑100 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                     <  2:1492342/100‑1 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCCGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                     >  2:353122/1‑100 (MQ=255)
               gCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATc                                                                                  <  1:576597/100‑1 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                          <  1:525985/100‑1 (MQ=255)
                          aTTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTg                                                                       <  1:670543/100‑1 (MQ=255)
                            tttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGcc                                                                     <  1:325741/100‑1 (MQ=255)
                              tCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGa                                                                   >  2:1360686/1‑100 (MQ=255)
                               cAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaa                                                                  >  2:574492/1‑100 (MQ=255)
                               cAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaa                                                                  >  2:1334603/1‑100 (MQ=255)
                                  cTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                               >  2:470468/1‑100 (MQ=255)
                                    gcgcCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTCCAAACAGCCAGACATCAGTAAACACTGCCGAAAACAg                                                             >  1:175419/1‑100 (MQ=255)
                                     cgcCAGCCACGTCTAAACCAGTTGCGGGTTAGCGGGTTTACCCTCCTGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                               <  2:88621/97‑1 (MQ=255)
                                      gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt                                                           >  2:1555183/1‑100 (MQ=255)
                                       ccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGttt                                                          >  1:1001544/1‑100 (MQ=255)
                                         agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                        <  2:630094/100‑1 (MQ=255)
                                         agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                        <  1:998730/100‑1 (MQ=255)
                                         agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAAATCGGTAAACACTGCCGAAAACAGTTTGc                                                        <  2:395030/100‑1 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                       <  2:722191/100‑1 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                       <  1:1213748/100‑1 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                       <  2:1187232/100‑1 (MQ=255)
                                           ccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa                                                      <  1:37400/100‑1 (MQ=255)
                                             aCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATa                                                    >  1:481480/1‑100 (MQ=255)
                                               ttCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAAt                                                  <  1:871847/100‑1 (MQ=255)
                                                tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                 <  1:383683/100‑1 (MQ=255)
                                                tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                 >  1:273863/1‑100 (MQ=255)
                                                 ctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCt                                                <  2:387786/100‑1 (MQ=255)
                                                   naaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                              >  2:481914/2‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                              >  2:1353437/1‑100 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc                                             >  2:427375/1‑100 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGTAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                           >  2:1501023/1‑100 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTATTCAAACAGCCAGACATCGGTAAAGACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                           <  1:1497354/100‑1 (MQ=255)
                                                          ttGCGGGTTAGCCGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACGGTTTGCGATAATCTTCCGgttgt                                       <  2:702369/100‑1 (MQ=255)
                                                            gCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTc                                     <  1:1045270/100‑1 (MQ=255)
                                                            gCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTc                                     >  1:1301919/1‑100 (MQ=255)
                                                             cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg                                    <  1:574493/100‑1 (MQ=255)
                                                              gggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgc                                   >  2:209182/1‑100 (MQ=255)
                                                               ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc                                  <  1:375862/100‑1 (MQ=255)
                                                                gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg                                 >  1:603106/1‑100 (MQ=255)
                                                                 ttAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCAAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccgc                                <  1:360230/100‑1 (MQ=255)
                                                                  tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCt                               <  2:1360687/100‑1 (MQ=255)
                                                                  tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCt                               <  1:1431105/100‑1 (MQ=255)
                                                                   aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                              >  1:800323/1‑100 (MQ=255)
                                                                   aGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                              >  1:651561/1‑100 (MQ=255)
                                                                               cTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa                  <  2:435586/100‑1 (MQ=255)
                                                                               cTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa                  <  1:1154558/100‑1 (MQ=255)
                                                                                  cGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATcc               <  1:326316/100‑1 (MQ=255)
                                                                                  cGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATcc               <  1:326175/100‑1 (MQ=255)
                                                                                     ccccAGCAGTTGTTCAAACAGCCAGACAGCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGc            <  2:984879/100‑1 (MQ=255)
                                                                                        cagcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAg         >  2:159146/1‑100 (MQ=255)
                                                                                          gcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt       >  1:141431/1‑100 (MQ=255)
                                                                                             gttgttCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTc    >  1:1352172/1‑100 (MQ=255)
                                                                                               tGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc  >  1:256159/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGC  >  NC_000913/2307289‑2307483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑