| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,386,365 | T→A | F169Y (TTC→TAC) | yfbL → | putative M28A family peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,386,365 | 0 | T | A | 98.0% | 169.2 / ‑3.5 | 51 | F169Y (TTC→TAC) | yfbL | putative M28A family peptidase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (26/24); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
GTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAC > NC_000913/2386271‑2386459 | gtgGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTc < 2:976029/100‑1 (MQ=255)gtgGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTc > 1:1283917/1‑100 (MQ=255) ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGt > 2:1072814/1‑100 (MQ=255) gCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTa < 1:1310937/100‑1 (MQ=255) cAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGATCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAg < 2:174974/100‑1 (MQ=255) cAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACAGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAg < 2:175675/100‑1 (MQ=255) cGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATg > 2:442288/1‑100 (MQ=255) gAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATgg < 2:1283912/100‑1 (MQ=255) aaCTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATggg < 2:1481855/100‑1 (MQ=255) aCTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATgggg < 2:1249494/100‑1 (MQ=255) cTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGa > 1:1287069/1‑100 (MQ=255) ggCACGTTTGTTACATCAGCACGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgc > 2:291905/1‑100 (MQ=255) gCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgcg < 2:1212148/100‑1 (MQ=255) cGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCgg > 1:714621/1‑100 (MQ=255) gTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGt < 1:645228/100‑1 (MQ=255) tttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtg < 2:134001/100‑1 (MQ=255) tttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtg < 2:737559/100‑1 (MQ=255) ttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgc > 2:692243/1‑100 (MQ=255) ttGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgc < 2:801306/100‑1 (MQ=255) tGTTACATCAGCAAGGACCGAAAACAGGCGTGCAGCTGGTGGCCTATGCGTCGGAAGAGCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgca < 2:778932/100‑1 (MQ=255) gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat > 2:477018/1‑100 (MQ=255) tACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgc > 1:783105/1‑100 (MQ=255) aTCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGc < 1:1245248/100‑1 (MQ=255) aaGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCt > 2:1350981/1‑100 (MQ=255) gTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGTGCGGTGCATGCAGCTTCGCTTg > 2:513063/1‑100 (MQ=255) gTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTg < 2:1473836/100‑1 (MQ=255) aCCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg > 1:200665/1‑100 (MQ=255) cGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCg < 2:1027290/100‑1 (MQ=255) gAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGt > 1:637517/1‑100 (MQ=255) gAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGAGGATCGCGGTGCATGCAGCTTCGCTTGAGCGt > 2:1024834/1‑100 (MQ=255) ggCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGa > 2:1165784/1‑100 (MQ=255) cGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGaaa < 2:776185/100‑1 (MQ=255) cAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTaa > 1:395502/1‑100 (MQ=255) ggTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATa < 1:1376651/100‑1 (MQ=255) ggTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATa > 1:380172/1‑100 (MQ=255) ggTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATa < 2:251036/100‑1 (MQ=255) gTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGGGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAg < 2:783101/100‑1 (MQ=255) tCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGc > 2:1355850/1‑100 (MQ=255) cGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCa > 2:703752/1‑100 (MQ=255) cGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCa > 2:1194267/1‑100 (MQ=255) gCCTATGCTTCGGAAGAACCGCCCTATTTTCTTAGCGATGAAATGTGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCAc < 2:1208711/100‑1 (MQ=255) ggcGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatg > 1:1060231/3‑100 (MQ=255) gTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatg < 2:379896/100‑1 (MQ=255) tCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatga > 1:376181/1‑100 (MQ=255) ggAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGAtt > 1:1370897/1‑100 (MQ=255) ggAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGAtt < 1:721507/100‑1 (MQ=255) aaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTa > 2:1260230/1‑100 (MQ=255) aCCGCCCTACTTTCGTAGCGATGAAATGGGGAGTGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAt < 1:513064/100‑1 (MQ=255) ccGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAtt > 2:1237341/1‑100 (MQ=255) gCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAc > 2:223789/1‑100 (MQ=255) gCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTACATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAc > 2:1442850/1‑100 (MQ=255) | GTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTAC > NC_000913/2386271‑2386459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |