Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,250,674 Δ1 bp 100% coding (958/1050 nt) yeiH → UPF0324 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,250,6710G.74.4% 261.3 / 143.1 121coding (955/1050 nt)yeiHUPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (13/18);  new base . (38/52);  total (51/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 127 total aligned reads displayed.

GCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAC  >  NC_000913/2250573‑2250768
                                                                                                  |                                                                                                 
gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTgg                                                                                                  <  1:1481332/100‑1 (MQ=255)
gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTgg                                                                                                  >  2:213702/1‑100 (MQ=255)
 cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTggg                                                                                                 <  1:3206343/100‑1 (MQ=255)
   tggtgAACATGCTGGTAACGCTGGATGCGTTCCTGCTGGCAATGGCGATGGCGGCGCGGAGTCTGACGACTCTCGTCAGCGCGCTGAAAAAAGCTgg                                                                                                  <  2:2653695/97‑1 (MQ=255)
   tggtgAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTgggcg                                                                                               <  1:1361734/100‑3 (MQ=255)
    ggtgAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTgggcga                                                                                              >  1:1813072/1‑97 (MQ=255)
       gAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGaaa                                                                                           <  1:502553/100‑1 (MQ=255)
           aagcaagTAACGCTGGATACCTTGCTGATAGCAACGGCGATGGCAACGCGGGGTCTGACCACTCATGTAAAAAAAAAAAAAAAAGCTGGGCGAAACctct                                                                                      <  2:1604421/94‑1 (MQ=255)
            tGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGTGGCGGGCTCTGACTACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTctg                                                                                     >  2:1937997/1‑100 (MQ=255)
            tGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACctct                                                                                      >  1:1506221/1‑100 (MQ=255)
            tGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACctct                                                                                      >  2:3461141/1‑99 (MQ=255)
            tGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACctct                                                                                      >  2:2103725/1‑99 (MQ=255)
              cTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTctgct                                                                                   >  1:2588465/1‑100 (MQ=255)
               tGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTctgct                                                                                   >  1:1775741/1‑100 (MQ=255)
                ggTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGa                                                                                 >  2:1994733/1‑100 (MQ=255)
                  tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGAt                                                                                <  1:2650909/100‑1 (MQ=255)
                  tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATg                                                                               <  2:2777835/100‑1 (MQ=255)
                    aCGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGc                                                                             <  1:1674037/100‑1 (MQ=255)
                     cGCTGGATCCCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGCCCACTCACGTCAGCGCGCTTAAAAAAGCTGGGGCGAAACCTCTGCTGATGGc                                                                             <  1:3961853/100‑1 (MQ=255)
                     cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCa                                                                            >  1:1049360/1‑100 (MQ=255)
                      gCTGGATAACTTCCTGCTGGCAATGGCGATGGAGGCGCTGGGTCTGACCACTCACGTTAGCACGCTAAAAAAAGCTGGGCGAAACCTCTGCTGATGGc                                                                             <  1:361550/98‑1 (MQ=255)
                       cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACt                                                                          >  1:913483/1‑100 (MQ=255)
                       cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACt                                                                          <  1:2946871/100‑1 (MQ=255)
                         ggATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTgg                                                                        <  2:1925483/100‑1 (MQ=255)
                           aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTa                                                                      >  2:169079/1‑100 (MQ=255)
                            tACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTAc                                                                     >  1:3487943/1‑100 (MQ=255)
                             aCCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTAAAAAAAAAAAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGt                                                                       <  2:1118756/98‑1 (MQ=255)
                              ccTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACt                                                                    >  1:3394595/1‑100 (MQ=255)
                              ccTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTg                                                                   >  1:490138/1‑100 (MQ=255)
                                ttCCTGCTGGCACTGGCGATGGCGGCGCTGGGTCTGTCGACTCACGTCAGCGCGCTGAAAATAGCTGGGCGAAACCTCTGCTGTTGGCACTGGTACTGt                                                                  <  2:2254087/99‑1 (MQ=255)
                                ttCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACTACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGt                                                                  <  2:2778153/100‑1 (MQ=255)
                                 tCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGtt                                                                 <  2:1372432/99‑1 (MQ=255)
                                  ccTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTg                                                               >  2:1534918/1‑100 (MQ=255)
                                   ctgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGc                                                              <  1:3347501/100‑1 (MQ=255)
                                   ctgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCGCTGCTGATGGCACTGGTACTGtt                                                                 >  2:3501773/1‑97 (MQ=255)
                                      ctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                           >  2:1189126/1‑100 (MQ=255)
                                       tgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                           <  1:3144834/100‑1 (MQ=255)
                                       tgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                          >  1:2413818/1‑100 (MQ=255)
                                       tgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                          >  2:4038849/1‑100 (MQ=255)
                                        ggCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGc                                                         <  1:2476695/100‑1 (MQ=255)
                                        ggCAACTGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCACTCTGAAAAAAGCTGTGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGc                                                         <  2:1713477/100‑1 (MQ=255)
                                          cAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                       <  1:1603431/100‑1 (MQ=255)
                                          cAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                       >  2:2959668/1‑100 (MQ=255)
                                            atggcgatggcgGCGCTGGGTCTGACCGCTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                       <  2:647291/98‑1 (MQ=255)
                                             tggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAtt                                                    >  1:2462408/1‑100 (MQ=255)
                                               gcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgt                                                  >  2:57469/1‑100 (MQ=255)
                                                cgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGATGAttgtt                                                 >  2:2629076/1‑100 (MQ=255)
                                                 gatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgttg                                                <  1:566772/100‑1 (MQ=255)
                                                 gatggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgttg                                                >  1:2890721/1‑100 (MQ=255)
                                                  atggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtgg                                               <  1:1628469/100‑1 (MQ=255)
                                                  atggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtgg                                               <  1:2236165/100‑1 (MQ=255)
                                                   tggcgGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggt                                              >  1:3796689/1‑100 (MQ=255)
                                                    ggcggcGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtg                                             <  1:3076066/100‑1 (MQ=255)
                                                     gcggcgCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtg                                             <  1:3718724/100‑1 (MQ=255)
                                                     gcggcgCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtgg                                            <  1:1189131/100‑1 (MQ=255)
                                                       ggcgCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggt                                           <  2:2404121/100‑1 (MQ=255)
                                                       ggcgCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                          <  1:4123132/100‑1 (MQ=255)
                                                        gcgcTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtgg                                         <  2:2471908/100‑1 (MQ=255)
                                                          gcTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAGAGCTGGGCGAAGCCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGgtggtggtg                                       <  2:1357652/100‑1 (MQ=255)
                                                           cTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                      <  1:754892/100‑1 (MQ=255)
                                                              ggTCTGACCACTCCCGTCAGCGCGCTGAAAAAAGCTGGTCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                   >  2:2572876/1‑100 (MQ=255)
                                                              ggTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                   <  2:2024610/100‑1 (MQ=255)
                                                               gTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGTGCGAAACCTCTGCTGATGGCACTGTTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                  <  2:853960/100‑1 (MQ=255)
                                                               gTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGATGCCTCTGCTGATGGGACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                  <  2:333966/100‑1 (MQ=255)
                                                               gTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                  >  1:1173458/1‑100 (MQ=255)
                                                                tCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAtt                                  >  1:3674111/1‑100 (MQ=255)
                                                                tCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTa                                 <  2:1813062/100‑1 (MQ=255)
                                                                  tGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaa                                >  1:2142730/1‑100 (MQ=255)
                                                                  tGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAAc                               >  1:2562597/1‑100 (MQ=255)
                                                                  tGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAAc                               <  1:4135848/100‑1 (MQ=255)
                                                                  tGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAAc                               <  1:669052/100‑1 (MQ=255)
                                                                   gaacACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAAc                               <  2:325567/97‑1 (MQ=255)
                                                                   gACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACt                              >  1:1318010/1‑100 (MQ=255)
                                                                    aCCACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTa                             <  1:3699204/100‑1 (MQ=255)
                                                                     ccACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTa                             >  1:635487/1‑100 (MQ=255)
                                                                     ccACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGCTTGTTGGTGGTGCTGCGCTTAACTAt                            >  2:507434/1‑100 (MQ=255)
                                                                      cACTCACGTCAGCGCGCTGAAAAAAGCTTGTCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                           <  1:3477255/100‑1 (MQ=255)
                                                                      cACTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                           <  1:311718/100‑1 (MQ=255)
                                                                       aCTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                           <  1:3278180/100‑1 (MQ=255)
                                                                        cTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtgtg                         <  1:1994743/100‑1 (MQ=255)
                                                                        cTCACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtgtg                         >  2:1028064/1‑100 (MQ=255)
                                                                          cACGTCAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAt                       <  1:2137303/100‑1 (MQ=255)
                                                                           aCGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAt                       <  2:482415/100‑1 (MQ=255)
                                                                            cGTCAGCGGGCTGAAACAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTATGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTc                     <  2:2438594/100‑1 (MQ=255)
                                                                            cGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAtt                      >  1:2332107/1‑100 (MQ=255)
                                                                            cGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAtt                      >  2:3853691/1‑100 (MQ=255)
                                                                               cAGCGCGCTGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaaa                  <  1:1832189/100‑1 (MQ=255)
                                                                                 gcgcgcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAg                 <  1:2996814/100‑1 (MQ=255)
                                                                                      cTGAAAAAATCTGGGCGAAACCTCTGCTAATGGCACTGGTACTGTTTGCATGGCTGATAGTTGGTGGTGGTGCGTTTAATTATGTGATTCAAAGCGTCa            >  2:2827846/1‑99 (MQ=255)
                                                                                      cTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCa            >  1:1662711/1‑100 (MQ=255)
                                                                                      cTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCa            >  2:133940/1‑100 (MQ=255)
                                                                                       tGAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc          >  1:964956/1‑100 (MQ=255)
                                                                                        gAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCg         <  1:52235/100‑1 (MQ=255)
                                                                                        gAAAAAAGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCg         >  1:533074/1‑100 (MQ=255)
                                                                                         aaaaaaGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGCGGTGCGATTAACTATGTGATTCAAAGCGTCATCGc        >  2:1211972/1‑100 (MQ=255)
                                                                                          aaaaaGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGc        <  2:1775731/100‑1 (MQ=255)
                                                                                          aaaaaGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCa       >  1:1250920/1‑100 (MQ=255)
                                                                                           aaaaGCTGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCAt      <  1:65996/100‑1 (MQ=255)
                                                                                                cTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAc  <  2:3147583/100‑1 (MQ=255)
                                                                                                cTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAc  >  1:3792386/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAC  >  NC_000913/2250573‑2250768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: